Your browser doesn't support javascript.
loading
Montrer: 20 | 50 | 100
Résultats 1 - 2 de 2
Filtre
Ajouter des filtres








Gamme d'année
1.
Indian J Biochem Biophys ; 2015 Feb; 52 (1): 101-106
Article Dans Anglais | IMSEAR | ID: sea-157961

Résumé

Four protocols viz., the trichloroacetic acid-acetone (TCA), phenol-ammonium acetate (PAA), phenol/SDS-ammonium acetate (PSA) and trisbase-acetone (TBA) were evaluated with modifications for protein extraction from banana (Grand Naine) roots, considered as recalcitrant tissues for proteomic analysis. The two-dimensional electrophoresis (2-DE) separated proteins were compared based on protein yield, number of resolved proteins, sum of spot quantity, average spot intensity and proteins resolved in 4-7 pI range. The PAA protocol yielded more proteins (0.89 mg/g of tissues) and protein spots (584) in 2-DE gel than TCA and other protocols. Also, the PAA protocol was superior in terms of sum of total spot quantity and average spot intensity than TCA and other protocols, suggesting phenol as extractant and ammonium acetate as precipitant of proteins were the most suitable for banana rooteomics analysis by 2-DE. In addition, 1:3 ratios of root tissue to extraction buffer and overnight protein precipitation were most efficient to obtain maximum protein yield.


Sujets)
Acétates/analogues et dérivés , Électrophorèse/méthodes , Musa/composition chimique , Phénylacétates , Protéines végétales/isolement et purification , Racines de plante/enzymologie , /méthodes
2.
Indian J Exp Biol ; 2014 Mar; 52(3): 252-260
Article Dans Anglais | IMSEAR | ID: sea-150355

Résumé

Activity differences of the first (phenylalanine ammonia lyase, PAL) and the last (cinnamyl alcohol dehydrogenase, CAD) enzymes of phenylpropanoid pathway in the roots of resistant (Yangambi Km5 and Anaikomban) and susceptible (Nendran and Robusta) banana cultivars caused by root lesion nematode, Pratylenchus coffeae, were investigated. Also, the accumulation of phenolics and deposition of lignin polymers in cell walls in relation to resistance of the banana cultivars to the nematode were analyzed. Compared to the susceptible cultivars, the resistant cultivars had constitutively significantly higher PAL activity and total soluble and cell wall-bound phenolics than in susceptible cultivars. The resistant cultivars responded strongly to the infection of the nematode by induction of several-time higher PAL and CAD enzymes activities, soluble and wall-bound phenolics and enrichment of lignin polymers in cell wall and these biochemical parameters reached maximum at 7th day postinoculation. In addition, profiles of phenolic acid metabolites in roots of Yangambi Km5 and Nendran were analyzed by HPLC to ascertain the underlying biochemical mechanism of bananas resistance to the nematode. Identification and quantification of soluble and cell wall-bound phenolic acids showed six metabolites and only quantitative, no qualitative, differences occurred between the resistant and susceptible cvs. and between constitutive and induced contents. A very prominent increase of p-coumaric, ferulic and sinapic acids, which are precursors of monolignols of lignin, in resistant cv. was found. These constitutive and induced biochemical alterations are definitely the chemical defenses of resistant cvs. to the nematode infection.


Sujets)
Alcohol oxidoreductases/génétique , Alcohol oxidoreductases/métabolisme , Animaux , Résistance à la maladie/génétique , Voies et réseaux métaboliques , Musa/enzymologie , Musa/génétique , Musa/croissance et développement , Musa/parasitologie , Nematoda/génétique , Nematoda/pathogénicité , Phénols/composition chimique , Phénols/métabolisme , Phenylalanine ammonia-lyase/génétique , Phenylalanine ammonia-lyase/métabolisme , Maladies des plantes/génétique , Maladies des plantes/parasitologie , Racines de plante/enzymologie , Racines de plante/métabolisme , Polymères/composition chimique , Propanols/composition chimique , Propanols/métabolisme
SÉLECTION CITATIONS
Détails de la recherche