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1.
Genet. mol. biol ; 23(4): 725-727, Dec. 2000. graf
Article Dans Anglais | LILACS | ID: lil-303638

Résumé

O polimorfismo de DNA é muito útil em pesquisa de paternidade. O presente trabalho descreve estudos de paternidade usando perfis de DNA obtidos com a sonda (CAC)5. Todos os indivíduos estudados estavam envolvidos em casos näo judiciais de paternidade. DNA genômico digerido com HaeIII foi colocado em gel de agarose e hibridizado no gel com a sonda (CAC)5 marcada com 32P. O número médio de bandas maiores que 4,3 kb por indivíduo foi 16,1. A proporçäo média de bandas compartilhadas entre indivíduos näo aparentados foi 0,08 e o número médio de bandas de teste foi 7,1. Isto correspondeu a uma probabilidade de exclusäo maior que 0,999999. A paternidade foi excluída em 34,5 por cento dos casos. A freqüência de mutaçäo estimada em casos näo excluídos foi 0,01143 bandas por criança. Nestes casos, a paternidade foi confirmada por uma análise locus-específica de oito locos independentes obtidos com PCR. O índice de paternidade foi computado em todos os casos näo excluídos. Pode-se concluir que este método é uma alternativa poderosa e econômica para resolver dúvidas de paternidade.


Sujets)
Humains , Mâle , Femelle , Enfant , Adulte , Paternité , Réaction de polymérisation en chaîne , Chili , Pedigree , Polymorphisme génétique
2.
Rev. méd. Chile ; 123(5): 560-6, mayo 1995. tab, ilus
Article Dans Espagnol | LILACS | ID: lil-152857

Résumé

Simultaneous detection of several VNTR loci using a single DNA probe is the basis of the technique called DNA fingerprint (DNAfp) of increasing application in parenthood identification. According to the data gathered by different laboratories worldwide, father exclusion can be made in a larger number of cases when compared with the customary tests based on erythrocyte antigens. The question could then be whether DNAfp will completely replace erythrocyte antigen tests. We report here our experience in applying DNAfp to 92 samples corresponding to 34 paternity cases and comparing these with the results obtained with the antigens of the systems ABO, Rh. MNSs, Duffy and Kidd. Most of the HaeIII/digested DNA samples produced 13 to 16 bands larger than 4,3 Kb (average 14,0761ñ2,205). Average band sharing between pairs of unrelated individual was 1,907ñ1,083. Two cases presenting an a posteriori probability of being the father of 80.7 percent and 76.5 percent by erythrocyte antigens were clearly excluded by DNAfp. All exclusions made by antigens were confirmed by DNAfp. In the cases reported as father probable (28 cases) by DNAfp, these shared with the child 6,7407ñ1,7 bands on average. Because of time, cost and simplicity we favor a procedure starting with the antigens test and continuing with DNAfp only when an exclusion is not possible. Economy will increase as the number of exclussions increases


Sujets)
Humains , Paternité , Profilage d'ADN , Polymorphisme génétique/génétique , Fréquence d'allèle/génétique , Antigènes de groupe sanguin/génétique
3.
Rev. méd. Chile ; 122(9): 1009-14, sept. 1994. tab
Article Dans Espagnol | LILACS | ID: lil-138043

Résumé

The aim of this work is to analyze the distribution of Duffy blood group and the reproductive history of 148 malformed newborns and their mothers compared to 131 control pairs. The mother -child segregation of the system is analyzed using ITO matrixes. A higher frequency of heterozygote mothers for the system was found among tha malformed group compared to controls. No differences in the reproductive history was found between Duffy system homozygote or heterozygote mothers


Sujets)
Nouveau-né , Adulte , Malformations/sang , Système Duffy/génétique , Phénotype , Études cas-témoins , Fréquence d'allèle/génétique , Mères
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