Your browser doesn't support javascript.
loading
Montrer: 20 | 50 | 100
Résultats 1 - 1 de 1
Filtre
Ajouter des filtres








Gamme d'année
1.
Mem. Inst. Oswaldo Cruz ; 98(3): 373-376, Apr. 2003. ilus
Article Dans Anglais | LILACS | ID: lil-340116

Résumé

The use of in situ techniques to detect DNA and RNA sequences has proven to be an invaluable technique with paraffin-embedded tissue. Advances in non-radioactive detection systems have further made these procedures shorter and safer. We report the detection of Trypanosoma cruzi, the causative agent of Chagas disease, via indirect and direct in situ polymerace chain reaction within paraffin-embedded murine cardiac tissue sections. The presence of three T. cruzi specific DNA sequences were evaluated: a 122 base pair (bp) sequence localized within the minicircle network, a 188 bp satellite nuclear repetitive sequence and a 177 bp sequence that codes for a flagellar protein. In situ hybridization alone was sensitive enough to detect all three T. cruzi specific DNA sequences


Sujets)
Animaux , Souris , ADN des protozoaires , Coeur , Trypanosoma cruzi , ADN kinétoplastique , ADN des protozoaires , Hybridation in situ , Réaction de polymérisation en chaîne , Trypanosoma cruzi
SÉLECTION CITATIONS
Détails de la recherche