role of black seed and/or bees honey in modulating the heart disorder induced by food additives in male rats

The present study aimed to throw the light on the potential hazards of some food additives whether preservatives such as sodium nitrite or colors as sunset yellow on the heart disorders through their own effect or the interaction between them. The study also, investigated the possible protective role of black seed and/or bees honey against such risks. Herein, the mentioned food additives caused significant elevations in serum and cardiac total lipids [TL], total cholesterol [TC], triglycerides [TG] and phospholipids [PL] levels as well as serum LDL-c, VLDL-c levels and LDL-c/HDL-c ratio. Meanwhile, significant decrements were shown in serum HDL-c level and HDL-c/TC ratio. Additionally, creatine kinase [CK] and aspartate transferase [AST] enzymes activity were increased significantly in serum and cardiac tissue. Moreover, oxidative stress markers; lipid peroxidation product [MDA] and protein carbonyl group [PC] were increased significantly in the cardiac tissue. Regarding, antioxidants glutathione [GSH] content, glutathione -S -transferase [GST] and catalase [CAT] enzymes activity were decreased significantly in the cardiac tissue. However, the use of black seed and/or bees honey ameliorated the disturbances observed indicating remarkable protection against the toxic effects of these food additives on the heart and offers more safety. Overall, here with the most pronounced effect was achieved by the combined treatment. In addition, the treatment by bees honey was more effective than black seed

Biochemical and histochemical changes induced by long term use of nalbuphine versus morphine in rat liver

Fifty-six mature albino rats were used for this study. They were divided into three groups. Eight animals were kept as placebo control group [group C] and were injected saline intraperitoneally, daily for one month. The remaining 48 animals were divided into 2 main groups, 24 animals each: Nalbuphine group [group N] and morphine group [group M]. Each was subdivided into 3 equal subgroups, 8 animals each. Each subgroup was given the corresponding drug in the analgesic dose [N1 and M1 subgroups given 0.9 ug/g b. wt.], double the analgesic dose [N2 and M2 subgroups given 1.8 ug/g b. wt.] or triple the analgesic dose [N3 and M3 subgroups given 2.7 ug/g b. wt.] intraperitoneally, daily for one month. Alanine transferase [ALT], aspartate transferase [AST], alkaline phosphatase [ALP], total proteins and albumin were estimated after one month. Histochemical studies of ALP, acid phosphatase [ACP] and succinic dehydrogenase were done. From the results obtained it was concluded that nalbuphine hydrochloride is much more safer than morphine for prolonged use, especially in patients with compromised livers