Immunoglobulin therapy in recurrent pregnancy loss

Recurrent pregnancy loss is a global issue. This study was planned in order to evaluate the effects of high dose intravenous immunoglobulin [IVIg] in primary and secondary unexplained miscarriages in patients of recurrent pregnancy loss. Total 168 couples having history of primary or secondary recurrent pregnancy loss were included in the study. They were divided into IVIg group and control group. IVIg group was given 50 gm of IVIg on monthly basis. Control group was provided with normal saline drip at same intervals. Statistical package for social sciences 19 [SPSS 19] was used to analyze the data. Women receive IVIg treatment [primary + secondary recurrent pregnancy loss] shows significantly higher percentage of live birth as compare to control group [81%; p < 0.000]. Percentage of full term live birth is also significantly increased in IVIg group in comparison with control group [76.2%; p < 0.000]. In primary recurrent pregnancy loss total percentage of live births and full term live births were 85.3 and 79.4% in IVIg group while in control group it was 26.9% and 19.2% [p < 0.000]. In secondary recurrent pregnancy loss percentage of total number of live birth and full term live births in IVIg group is 78% and 74% in comparison with control group 32% and 26% respectively [p < 0.000]. High dose intravenous immunoglobulin has a beneficial role in patients of primary and secondary recurrent miscarriages

Role of female age in determining causes of infertility and predicting outcome of in vitro fertilization

To look into various causes of infertility according to increasing age, differences in percentage of pregnancy rate after IVF in different age groups and role of various infertility parameters in determining pregnancy rate among these patients. A total of 206 infertile couples were selected and divided into two groups according to female age: Group I [<35 years, n=108] and Group II [>/= 35 years, n=98]. Diagnostic hysterosalpingography or laproscopy was done in order to rule out the tubal blockage and endometriosis. Ovarian dysfunction was excluded on the basis of hormonal analysis, IVF treatment following a long protocol for use of GnRH agonist was given to infertile couple. Analysis was done using SPSS 19 and MedCal software 12. Male causes were frequent in both groups without any significant difference [33%; p=0.882]. Tubal factor was significantly raised in older group [33%; p=0.000], whereas ovarian dysfunction was significantly higher in younger patients [33%; p=0.000]. Number of embryos transferred and pregnancy rate after IVF were significantly decreased in older couples [p

Effects of delta-tocotrienol and polyphenol on ageing induced oxidative stress and inflammation in humans

To evaluate the synergistic effects of delta-tocotrienol and polyphenols on ageing induced oxidative stress and inflammation on healthy elderly humans. It was a randomized double blinded control trial. Total 69 healthy volunteers aged 50-78 years consisting of 47 male and 22 female were included after informed consent. All the subjects were screened at baseline and randomly grouped in A and B. Nutritional supplement [delta-tocotrienol 200mg, Quercetin 300 mg, Resveratrol 100mg] and placebo [Sucrose] were given to groups A and B subjects respectively for 4 weeks. Then subjects of two groups were crossed over for next 4 weeks. Serum total antioxidant status [TAS] and Gamma glutamyl transferase [GGT] were analyzed by using Randox kits [UK] on automatic Chemistry analyzer Selectra E. Estimation of serum Nitrate was done on ELISA by using Kit [Cayman, UK]. Serum hS-C-recative protein [CRP] was analyzed by using chemiluminescent immunometric assay kit on Immulite 1000. There was significant reduction in pre versus post median [IQR] levels in serum GGT 26 [21-34] vs 21 [19-25] U/L after nutritional supplement [p=0<0.01]. Serum CRP and Nitrate levels were also significantly decreased from 2.1[1.1-3.1] and 8.30[3.52-16.20] to 1.4[0.75-2.3] mg/L and 7.65[3.74-11.58] micro mol/L respectively [p=0<0.01]. TAS was improved from 1.45 [1.33-1.62] to 1.61 [1.46-1.70] mmol/L with nutritional supplement [p=0<0.01]. The study has demonstrated that combination of tocotrienol and polyphenol delays the ageing process by reducing the deteriorating effects of oxidative stress and inflammation