Résumé
Objective:Flavonoids,as the major constituents in Oroxyli Semen,had a variety of pharmacological effects against inflammation,infections,and oxidation. In this study,an ultra-high performance liquid chromatography (UPLC-Q-TOF-MS) method was established to analyze and identify the various flavonoids in Oroxyli Semen. Method:The separation was performed on an Kinetex-XB C18 column(2.1 mm×50 mm,1.7 μm)with gradient elution,which was carried out with 0.1%formic acid acetonitrile as mobile phase. The flow rate was 0.25 mL·min-1,and the injection volume was 3 μL. Electrospray ionization source was in the negative ion mode. Target and non-target screenings were carried out by Peakview 2.2 and Masterview 1.0 software. Then the identification of target compounds were completed based on accurate mass,isotopic abundance ratio and MS/MS fragment,and the non-target compounds were determined according to literature reports and MS cleavage mechanism. Result:According to the high resolution MS spectra data,fragmentation ion information and retention time,36 peaks of Oroxyli Semen were identified, including 10 flavonoids and 26 flavonoid glycosides according to literature reports and MS cleavage mechanism. Conclusion:The study comprehensively analyzes the chemical composition of Oroxyli Semen,which is significant to study of the chemical constituents, quality control and material basis of Oroxyli Semen.