Résumé
@#[Abstract] Objective: To investigate the impact of FBXW7 gene mutation on cell proliferation, apoptosis, migration, and invasion processes of colorectal cancer HCT-116 cell line. Methods: Recombinant plasmids carrying wild-type and mutant-type FBXW7 SNP were constructed and transfected into HCT-116 cell line; the FBXW7 protein expression level in HCT-116 strains after transfection was detected by Western blotting. Subsequently, cell proliferation capacity was tested by CCK-8 assay; tumor cell colony formation ability was tested by HTCA; cell apoptosis function was tested by FCM; cell migration and invasion were tested by scratch assay and Transwell assay, respectively. Results: Higher HBXW7 protein expression level was detected in HCT-116 strain transfected with wild-type HBXW7 in comparison to the control group (strains transfected with mutant-type HBXW7), negative-control (strains transfected with empty plasmids), and blank-control (strains untransfected) (all P<0.05). Compared with the other groups, strains transfected with wildtype HBXW7 exhibited significantly reduced proliferation, colony formation, migration and invasion ability (all P<0.05), but obviously increased apoptosis rate (P<0.05). Conclusion： ：FBXW7 gene mutation can down-regulate its protein expression, and further promote the proliferation, migration and invasion as well as inhibit the apoptosis of HCT-116 cells.
Résumé
The study is aimed to estimate the effect of different heavy metals such as Hg2+, Cd2+, Mn2+, Cr3+, Ni2+, Se2O3(2-), As2O3 water solution and combined effects of Hg2+, Mn2+, Cr3+ on lipid peroxidation in mice liver homogenate in vitro. Lipid peroxidation was determined as thiobarbituric acid-reacting materials (TBA). We select five different concentrations of selected ions for experiments. Correlations used to identify the concentration of ions associated with lipid peroxidation. The rate of lipid peroxide formation in mice liver homogenate increased with the gradual addition of alcohol. When alcohol dose was up to 0.5 ml, the rate of lipid peroxide formation was greatest. At tested concentrations, the effects of metal ions on lipid peroxidation induced by alcohol were classified into three groups, and are as follows: (1) simulative, Hg2+. (2) inhibitory, Mn2+, Cr3+, Ni2+, Se2O3(2-). (3) ambiguous, Cd2+, As2O3 water solution. When Hg2+, Mn2+ and Cr3+ were added to the mice liver homogenate with alcohol at the same time, Hg2+, Mn2+ were the main agents for the rate of alcohol induced lipid peroxidation. The simulative effect of Hg2+ on lipid peroxidation induced by alcohol indicate that alcohol-drinkers will have further health risk when they are exposed in polluted regions than others, and Mn2+, Cr3+, Ni2+, Se2O3(2-) may act as free radical scavengers and preventive remedy for alcoholism in part. Furthermore, analysis of combined effect of Hg2+Mn2+ and Cr3+ provide us a new way to estimate the combined effect of multi-materials.