20-Hydroxyecdysone Improves Neuronal Differentiation of Adult Hippocampal Neural Stem Cells in High Power Microwave Radiation-Exposed Rats / 生物医学与环境科学（英文）

Objective@#The hippocampus is thought to be a vulnerable target of microwave exposure. The aim of the present study was to investigate whether 20-hydroxyecdysone (20E) acted as a fate regulator of adult rat hippocampal neural stem cells (NSCs). Furthermore, we investigated if 20E attenuated high power microwave (HMP) radiation-induced learning and memory deficits.@*Methods@#Sixty male Sprague-Dawley rats were randomly divided into three groups: normal controls, radiation treated, and radiation+20E treated. Rats in the radiation and radiation+20E treatment groups were exposed to HPM radiation from a microwave emission system. The learning and memory abilities of the rats were assessed using the Morris water maze test. Primary adult rat hippocampal NSCs were isolated in vitro and cultured to evaluate their proliferation and differentiation. In addition, hematoxylin & eosin staining, western blotting, and immunofluorescence were used to detect changes in the rat brain and the proliferation and differentiation of the adult rat hippocampal NSCs after HPM radiation exposure.@*Results@#The results showed that 20E induced neuronal differentiation of adult hippocampal NSCs from HPM radiation-exposed rats via the Wnt3a/β-catenin signaling pathway in vitro. Furthermore, 20E facilitated neurogenesis in the subgranular zone of the rat brain following HPM radiation exposure. Administration of 20E attenuated learning and memory deficits in HPM radiation-exposed rats and frizzled-related protein (FRZB) reduced the 20E-induced nuclear translocation of β-catenin, while FRZB treatment also reversed 20E-induced neuronal differentiation of NSCs in vitro.@*Conclusion@#These results suggested that 20E was a fate regulator of adult rat hippocampal NSCs, where it played a role in attenuating HPM radiation-induced learning and memory deficits.

Effect of hyperbaric oxygen treatment on expression of nestin in a model of right middle cerebral artery occlusion in rabbits / 中华神经医学杂志

Objective To evaluate the effect of hyperbaric oxygen (HBO) treatment on the expression of nestin in a model of right middle cerebral artery occlusion (MCAO) in rabbits. Methods Seventy healthy rabbits were dividod into 3 groups: the rabbits in MCAO+HBO group(n=30) were treated with HBO immediately after MCAO, once a day, consecutively for 20 d; the rabbits in MCAO group (n=30) were not trcated with any therapy after MCAO; the control group (n=10) received the same operation as that of MCAO group except MCAO. Rabbit behaviors and volumes of infarction were observed, and the expression of nestin was measured by immunocytochernistry 10 and 20 d after MCAO. Results Behaviors' scores and volumes of infarction were better in MCAO+HBO group than the MCAO group (P<0.05). 10 d after MCAO, the number of nestin-immunoreactive cells of MCAO+HBO group and MCAO group was respectively 15.88±1.2 and 6.63±1.6; 20 d after MCAO, the number in MCAO+HBO group and MCAO group was respectively 20.03±1.6 and 6.82±0.8. The number of nestin-immunoreactive cells in MCAO+HBO group was significantly higher compared with that of MCAO group (P<0.05). Conclusion Early applications of HBO and repeated administration of HBO for 20 d can evidently improve the behaviors and infarct size and elevate the expression of nestin in rabbit models of MCAO.