Résumé
ObjectiveTo optimize the extraction technology of the active component,rosmarinic acid,an ester of caffeic acid and 3,4-dihydroxyphenyllactic acid,in perilla oil meal for the first time by a new homogenizing technology called smashing tissue extraction (STE).MethodsOrthogonal design was used to optimize the extraction condition.The content of rosmarinic acid was quantified from the methanol crude extract with the help of HPLC.ResultsThe optimization of STE process to get rosmarinic acid from the perilla oil meal was the ratio of liquid to solid material at 10∶1 and the power of extraction at 150 V,extracting twice (2 min for each time).ConclusionSTE could be applied to extracting the active ingredients from the oil meals due to its high extraction efficiency.This new homogenizing technology has advantages on saving extraction time,raising extraction efficiency,and maintaining the temperature sensitive constituents.