Eucalyptus ESTs involved in the production of 9-cis epoxycarotenoid dioxygenase, a regulatory enzyme of abscisic acid production

Abscisic acid (ABA) regulates stress responses in plants, and genomic tools can help us to understand the mechanisms involved in that process. FAPESP, a Brazilian research foundation, in association with four private forestry companies, has established the FORESTs database (https://forests.esalq.usp.br). A search was carried out in the Eucalyptus expressed sequence tag database to find ESTs involved with 9-cis epoxycarotenoid dioxygenase (NCED), the regulatory enzyme for ABA biosynthesis, using the basic local BLAST alignment tool. We found four clusters (EGEZLV2206B11.g, EGJMWD2252H08.g, EGBFRT3107F10.g, and EGEQFB1200H10.g), which represent similar sequences of the gene that produces NCED. Data showed that the EGBFRT3107F10.g cluster was similar to the maize (Zea mays) NCED enzyme, while EGEZLV2206B11.g and EGJMWD2252H08.g clusters were similar to the avocado (Persea americana) NCED enzyme. All Eucalyptus clusters were expressed in several tissues, especially in flower buds, where ABA has a special participation during the floral development process

Comportamento de chamamento de Thyrinteina arnobia (Stoll) (Lepidoptera: Geometridae) em Psidium guajava, Eucalyptus grandis e em dieta artificial / Calling behavior of Thyrinteina arnobia (Stoll) (Lepidoptera: Geometridae) reared on Psidium guajava and Eucalyptus grandis host-plant leaves and on artificial diet

This work was carried out to determine and compare the effects of three diets (Psidium guajava and Eucalyptus grandis host-plant leaves and artificial diet) on the calling behavior and on thetemporal pattern of calling behavior of Thyrinteina arnobia (Stoll). All activities of virgin females during six scotophases were observed in the laboratory at 5min. intervals until the females stoppedcalling in the following photophase, at 22 ± 1oC, 70 ± 5% RH and 10h scotophase. The diets affected the calling behavior of T. arnobia. Two positions of calling were observed. Calling of female virgins started in the first hour immediately after emergence. The calling pattern was characteristic of the continuous pattern. The type of diet affected the mean onset of calling time (pre-calling) and the total time calling ofT. arnobia. Older females called earlier than younger after the onset of scotophase. The diets affected the color of pheromone gland.

Este estudo teve como objetivos determinar e comparar o efeito de três dietas: Psidium guajava, Eucalyptus grandis e dieta artificial no comportamento de chamamento e no padrão temporal do comportamento de chamamento de fêmeas virgens de Thyrinteina arnobia (Stoll). Foram analisados os parâmetros: tempo médio para o início do chamamento, número médio de chamamento, tempo médiode cada chamamento e tempo médio total de chamamento. Os bioensaios relacionados ao comportamento de chamamento de fêmeas virgens foram realizados durante seis escotofases consecutivas e as observações tomadas a cada 5 min., a 22 ± 1oC, 70 ± 5% de UR e 10h de escotofase. As fêmeas,independente da dieta analisada, apresentaram duas posições diferentes de chamamento. A maioria das fêmeas virgens iniciou o chamamento na 1a hora da 1a escotofase. O padrão de chamamento da fêmea individualizada foi característico de um padrão contínuo. O tipo de dieta oferecida na fase larvalinfluenciou o início do comportamento de chamamento (pré-chamamento) e o tempo médio total de chamamento. O tipo de dieta também alterou a coloração da glândula de feromônio.