Résumé
Objective To examine the effect of teaching surgical nursing using a team-based (TBL) combined with case-based learning (CBL) model on higher vocational students. Methods A total of 227 nursing students were investigated, and they were divided into the experimental group (n=97) and the control group (n=130) by simple random sampling. The TBL and CBL was applied in the experi-mental group, and the traditional 1ecture-based learning (LBL) was used in the control group. After the experiment teaching, a test and questionnaire were used to compare the teaching effect of experimental group and the control group. The data was analyzed by Independent-Samples T test and Chi-square test. Results The 97 students in the experimental group achieved higher mean test scores (89.18 ± 7.808) compared with the other participants' (85.53±11.033) in the control group and the difference was statistically significant (t=2.926, P=0.004). Assessments on participants' abilities, such as indepen-dent thinking, teamwork, communication, problem solving, active learning and so on, were significantly higher in the experimental group compared with the control group students and the difference was sta-tistically significant (P=0.000). Majority of the respondents (91students, 93.82%) noted that case-based, team-based learning was a better learning strategy compared to lecture-based learning. Most of the students (90 students, 92.78%) agreed that more such instruction activity should be organized in the future. Conclusions TBL combined with CBL has enhanced the students' learning achievement and improved their abilities of independent thinking, teamwork, communication, problem solving, autonomous learning and so on, and is welcome by the teachers and students.
Résumé
Mutated K-RAS is found in 15%～30% NSCLC patients, the mutations of K-RAS and EGFR are mutually exclusive,K-RAS mutations have poor sensitivity to EGFR-TKIs. The response of C-225 is not restricted to mutated K-RAS, NSCLC patients with K-RAS mutations are associated with unfavorable prognosis. A number of different approaches such as Ftase inhibitors, RAF inhibitors, MEK inhibitors aimed at abrogating K-RAS activity to improve clinical response have been explored in clinical trials.
Résumé
Interferons (IFNs) are natural proteins produced by wide variety of cells in response to viral infection or other biological inducers, and they execute diversified functions as antiviral defense, immune activation and cell growth regulation. Four genes encoding porcine interferons (PoIFN), PoIFN-alpha, PoIFN-gamma, PoIFN-alphagamma or PolFN-omega, were cloned and sequenced. The four types of porcine interferon genes were subcloned into the pET-His vector, and expressed in Escherichia coli Rosetta (DE3). The recombinant products were purified and renaturalized from inclusion bodies to obtain a native state of well biological activity. Antiviral activity assays for porcine interferons were performed and evaluated by standard procedures in following cell/virus test systems: Marc-145/PRRSV, Marc-145/VSV, PK-15/VSV, Vero/VSV or MDBK/VSV. The data showed that both PoIFN-alpha and PoIFN-alpagamma demonstrated significant antiviral activities, and the titer of them against PRRSV was up to 10(8) U/mg. PoIFN-gamma had approximately half or one-thirds antiviral activity of PoIFN-alpha. PoIFN-omega showed inconspicuous antiviral activity.