Résumé
Objective To investigate the clinical significance of combined detection of serum pepsinogen (PG) and anti-Hp IgG antibody in early screening for gastric ulcer .Methods 65 patients confirmed as gastric ulcer for the first time were selected as ulcer group ,while 65 healthy people in the same period as the control group .Transmission detection using latex enhanced immune nephe-lometry was adopted to detect their serum PG Ⅰ ,PG Ⅱ and the PG Ⅰ /PG Ⅱ ratio (PGR) was calculated .Enzyme-linked immu-nosorbent assay(ELISA) was used to detect serum anti-Hp IgG antibody .Results The average concentration of serum PG Ⅰand PG Ⅱof patients in the ulcer group were (167 .14 ± 22 .43) ,(26 .68 ± 11 .24) ng/mL ,respectively ,and PGR was 8 .07 ± 1 .19 .The average concentration of serum PG Ⅰand PG Ⅱof subjects in the control group were (52 .15 ± 20 .17) ,(11 .26 ± 7 .68) ng/mL ,re-spectively ,and PGR was 3 .48 ± 1 .92 .The differences of PGⅠ ,PGⅡand PGR between the two group showed statistical significance (P<0 .05) .The positive rate of serum anti-Hp IgG antibody of patients in the ulcer group was 80 .5% ,which was obviously higher than that in the control group(26 .3% )(P<0 .05) .The serum PGⅠ ,PGⅡand PGR of subjects in Hp infection positive group were markedly higher than those in the negative group(P<0 .05) .Conclusion Combined detection of serum PG and anti-Hp IgG anti-body may be used as an screening indicators for early gastric ulcer .
Résumé
Objective To investigate the relationship between Helicobacter pylori (H. pylori) infection and concentration of reactive oxygen species (ROS) in AGS cells. Methods AGS cells were cultured with either Hp11638 (CagA~+ , VacA~+ ) extract or Hp11638 mutant (CagA~+ , VacA~-) extract for 48 hours, then the cells and supernatants were collected. The concentration of ROS in AGS cells was measured by flow cytometry. The eytochrome C reduction was detected by spectrophotometer at 550 nm. Results The ROS levels in the AGS cells were correlated with two H. pylori strains in a concentration- and time-dependent manner. The ROS levels in AGS cells treated with Hp11638 extract in different concentrations or times were correspondingly higher than those treated with Hp11638 mutant extract. Similar results were found in examination of cytochrome C reduction. Conclusion The elevation of ROS in AGS cells is related to effects of H. pylori proteins, and the VaeA protein involves in the process.