Résumé
Objective To introduce the application experience of Leica CM1950 automatic constant temperature freezing microtome in frozen section.Methods Cooling time and temperature were determined based on the natures of submitted masses,and some techniques were involved in the rapid section staining and diagnosis.Results The submitted masses after using this microtome gained advantages in freezing speed,few ice crystal,easy operation and high quality,and facilitated accurate pathologic diagnosis.Conclusion Leica CM1950 automatic constant temperature freezing microtome has double compressor and semiconductor refrigeration units,can set corresponding freezing temperature for different organizations and provide high-quality frozen section for accurate and rapid intraoperative pathological diagnosis.