Résumé
Gas chromatography/mass spectrometry, chemometric resolution method ( CRM) and overall volume integration method were used to analyze the essential components of herbal pair Herba Ephedrae-Ramulus Cinnamomi (HP HE-RC) and compare it with that of single herbs HE and RC. 72, 68, and 97 essential components in essential oil of HE, RC, and HP HE-RC were determined, accounting for 90. 08% , 91. 62% , and 89. 76% of total contents of essential oil of HE, RC, and HP HE-RC, respectively. The numbers of essential components of HP HE-RC are almost the summation of that of two single herbs, but some relative contents of them are changed. Some new components, such as 1, 6-dimethylhepta-1,3,5-triene, tetracyclo[4. 2. 1.1 (2,5 ). 0 (9,10) ] deca-3,7-diene, globulol, ( E, E) -6, 10,14-trimethyl-5,9,13-pentadecatrien-2 -one, etc. have been found in HP HE-RC because of chemical reactions and physical changes during the course of decocation.
Sujets)
Techniques de chimie analytique , Méthodes , Cinnamomum , Chimie , Association médicamenteuse , Ephedra sinica , Chimie , Chromatographie gazeuse-spectrométrie de masse , Méthodes , Huile essentielle , Chimie , Huiles végétales , Chimie , Reproductibilité des résultatsRésumé
Analysis of common volatile constituents in herbal pair (HP) Chuanxiong Rhizome (CXR)-Notoperygium root (NR) and its single herb was performed by the method of alternative moving window factor analysis (AMWFA). In total, 65, 71, and 79 volatile chemical components in volatile oil of CXR, NR, and HP CXR-NR were separately determined qualitatively and quantitatively, accounting for 83.69%, 96.04% and 95.39% total contents of volatile oil of CXR, NR, and HP CXR-NR respectively. Analysis by the method of AMWFA indicates that there are 45 common volatile constituents in HP CXR-NR and single herb CXR, and 63 common volatile constituents in HP CXR-NR and single herb NR and 31 common volatile constituents among these three systems. The experimental results also show that the number of volatile chemical components in HP CXR-NR is almost equal to the sum of the number in the two single herbs, and volatile chemical components in HP CXR-NR are mainly from single herb NR.