Effect of Virtual Reality on Therapeutic Pain in Children with Spastic Cerebral Palsy / 中国康复理论与实践

Objective:To observe the effect of virtual reality on therapeutic pain in children with spastic cerebral palsy, through the profiles of the pain stress and outcome of rehabilitation treatment. Methods:From September, 2018 to June, 2019, 49 children with spastic cerebral palsy were randomly divided into control group (n = 25) and observation group (n = 24). Both groups received conventional rehabilitative treatment. The observation group wore virtual reality head display, choosing appropriate panoramic cartoon play or head control game according to their intelligence level to immerse themselves in the virtual environment. Both groups were treated five days per week for three weeks, for a total of 15 treatment days. Saliva cortisol was measured same time on the first, fifth, ninth, and 13th treatment days. They were assessed with modified Ashworth Scale (MAS), the Chinese version of Gross Motor Function Test Scale (GMFM) and Pediatric Disability Assessment Scale (PEDI) before and three weeks after treatment. Results:The cortisol levels significantly increased on all the treatment days in both groups (|t| > 3.502, P < 0.05). On the fifth, ninth, and 13th treatment days, the cortisol level was lower in the observation group than in the control group (t > 2.224, P < 0.05). After treatment, the MAS score decreased (|Z| > 2.636, P < 0.01), and the scores of PBS and PEDI increased (|Z| > 3.629, P < 0.001) in both groups; the scores of PBS and PEDI were higher in the observation group than in the control group (|Z| > 2.000, P < 0.05) Conclusion:Virtual reality is helpful to alleviate the pain stress in children with spastic cerebral palsy, and it is helpful to improve the effect of rehabilitation treatment.

Expression of S100A4 in esophageal squamous cell carcinoma and its relation to tumor invasion and metastasis / 南方医科大学学报

<p><b>OBJECTIVE</b>To study the role of S100A4 in the carcinogenesis, development, invasion and metastasis of esophageal squamous cell carcinoma.</p><p><b>METHODS</b>Immunohistochemistry was used to detect the expressions of S100A4, MMP-2 and E-cadherin proteins in 100 cases of surgically resected esophageal squamous cell carcinoma specimens. RT-PCR and Western blot were used to detect the expressions of S100A4 mRNA and protein in esophageal squamous cell carcinoma line EC-1 and TE-1. Boyden-chamber model in vitro was utilized to detect the invasion ability of EC-1 and TE-1 cells.</p><p><b>RESULTS</b>The positivity rate of S100A4 protein was 52.0% was in esophageal carcinoma tissues, significantly higher than that in normal tissues (26.0%) (P<0.01). The expression of S100A4 was related to tumor grading, invasive depth and lymph node metastasis (P<0.05). In esophageal carcinoma, the expression of S100A4 was positively correlated to MMP-2 expression (P<0.01), but inversely to E-cadherin expression (P<0.05). The expressions of S100A4 mRNA (0.894-/+0.021) and protein (0.897-/+0.053) in EC-1 cells were significantly higher than those in TE-1 (0.812-/+0.040 and 0.645-/+0.089, respectively, P<0.01), and the invasion ability of EC-1 cells was significantly higher than that of TE-1 cells (91.00-/+17.44 vs 61.80-/+11.10, P<0.01).</p><p><b>CONCLUSION</b>The overexpression of S100A4 in esophageal squamous cell carcinoma tissue and highly invasive EC-1 cells may contribute to the carcinogenesis, development, invasion and metastasis of esophageal squamous cell carcinoma.</p>

Effect of KISS-1 on invasive potential and proliferation of esophageal squamous carcinoma cell line EC-1 / 中华病理学杂志

<p><b>OBJECTIVE</b>To investigate the effect of KISS-1 expression on the potential of invasion and proliferation of esophageal squamous carcinoma cell EC-1.</p><p><b>METHODS</b>Protein and mRNA expressions of KISS-1 were evaluated by Western blot and RT-PCR in four esophageal carcinoma cell lines (EC-1, Eca109, EC9706 and TE-1). Using liposome-mediated transfection, an eukaryotic expression vector (pcDNA3.1-KISS-1) of KISS-1 gene was transfected into EC-1 cells. Boyden chamber model, MTT and clone formation assay were used to detect the potential of invasion and proliferation.</p><p><b>RESULTS</b>Western blot and RT-PCR showed a baseline low level of expression of KISS-1 protein (0.715 +/- 0.109) and mRNA (0.670 +/- 0.176) in EC-1 cells. pcDNA3.1-KISS-1 expression vector was successfully transfected into EC-1 cells. Western blot and RT-PCR showed that the expression of KISS-1 protein (1.143 +/- 0.218) and mRNA (0.877 +/- 0.162) in EC-1 cells transfected with pcDNA3.1-KISS-1 were significantly higher than those transfected with the control vector pcDNA3.1 (0.745 +/- 0.130, 0.685 +/- 0.128; t = 3.850, 2.481, P < 0.05) and the control cells (0.855 +/- 0.184, 0.677 +/- 0.138; t = 2.275, 2.306, P < 0.05). Boyden chamber analysis showed that the invasiveness of the cells transfected with KISS-1 at 24 h (91.8 +/- 11.7), 48 h (117.8 +/- 11.1) and 72 h (139.2 +/- 11.8) were significantly reduced than that of the cells transfected with the control vector pcDNA3.1 (118.1 +/- 14.7, 141.7 +/- 13.2, 162.2 +/- 22.7; t = 3.153, 4.215, 3.569, P < 0.01) and the control cells (112.2 +/- 15.6, 138.1 +/- 13.0, 162.3 +/- 14.0; t = 4.154, 3.797, 2.702, P < 0.05). MTT showed that the proliferation potential of cells after transfection with KISS-1 at 48 h (0.517 +/- 0.127) and 72 h (0.394 +/- 0.137) were significantly reduced than that of cells transfected with the control vector pcDNA3.1 (0.636 +/- 0.186, 0.513 +/- 0.150; t = 2.054, 2.709, P < 0.05) and the control cells (0.646 +/- 0.135, 0.511 +/- 0.153; t = 2.276, 2.205, P < 0.05). Clone formation assay suggested that cells transfected with KISS-1 (157.2 +/- 36.4) showed significantly decreased clone formation than cells transfected with the control vector pcDNA3.1 (236.3 +/- 78.1; t = 3.441, P < 0.01) and the control cells (242.5 +/- 48.6; t = 2.250, P < 0.05).</p><p><b>CONCLUSION</b>KISS-1 gene inhibits the potential of invasion and proliferation of EC-1 cells.</p>