Résumé
Objective The aim of this study is to explore the mechanism of canonical Wnt signaling pathway in type 2 diabetes mice peripheral neuropathy. Methods Male C57BL6 mice were randomly assigned to three groups. One group treated with normal diet as control. And the rest were used to establish the diabetic model through the combination of 60 kcal% high fat diet and an administration of multipleand low dose of streptozotocin on 5 consecutive days. When the model of type 2 diabetes mice peripheral neuropathy was successfully established, one group was injected with the canonical Wnt signaling pathway inhibitor XAV 939 ( T2DM-XAV 939 group) and the other one was injected with phosphate buffer saline (PBS) as control (T2DM-PBS group). The 21stweek was the end point of the experiment, and fasting blood glucose, insulin level, homeostasis model assessment for insulin resistance (HOMA-IR), plantar test, and exercise tolerance were measured, realtime PCR were adopted to detect the related mRNA expression of the canonical Wnt signaling pathway. Results T2DM-XAV 939 group had higher total cholesterol, triglyceride, fasting blood glucose, and HOMA-IR than T2DM-PBS group, but showed no statistical difference. The enzymatic activity of glutamic oxaloacetic transaminase was lower level than that in T2DM-PBS group (P＜0.05); T2DM-XAV 939 group had significantly higher plantar test and poorer exercise tolerance than those in T2DM-PBS group (P＜0.05). The mRNA levels of genes in canonical Wnt signaling pathway such as β-catenin, c-myc, mitochondrial transcription factor A (TFAM) had slightly lower level than those in T2DM-PBS group, without statistical difference, and the protein expression of c-myc was lower than that of T2DM-PBS group (P＜0.05). The insulin receptor substeate 2 (IRS-2) mRNA expression was higher than that in T2DM-PBS group (P＜0.05). With the development of the experiment, we found that the survival rate of the T2DM-XAV 939 group was significantly reduced compared with the other groups. Conclusion Inhibition of the canonical Wnt signaling pathway may aggravate diabetic peripheral neuropathy.
Résumé
Objective To develop and evaluate an aptamer based biosensor (aptasensor) for rapid colorimetric detection of enteropathogenic Escherichia coli (EPEC). Method The aptasensor was fabricated by modifying the truncated LPS-binding aptamer on the surface of nanoscale polydiacetylene vesicles using peptide bonding between the carboxyl group of the vesicle and the amine group of the aptamer. Molecular recognition between EPEC and aptamer at the interface of the vesicle led to blue-red transition of polydiacetylene which was readily visible to the naked eyes and could be quantified by colorimetric responses (CR). Transmission electron microscopy (TEM) was used to confirm the specific interactions between EPEC and polydiacetylene vesicles. Result Truncated aptamer showed the similar LPS-binding activity. The aptasensor could detect the target bacteria in a range of 105-108 colony-forming units (CFU)/ml within less than 30 minutes and its specificity was 100% for detection of EPEC O111. The sensor reproducibiliry obtained at 106 CFU/ml was 6. 08% R. S. D. The results of TEM confirmed that the specific interactions between EPEC and polydiacetylene vesicles. Conclusion A new aptasensor was developed successfully for rapid colorimetric detection of EPEC.
Résumé
C4 and Bf typing was performed by electroimmunofixation with antihuman C4 and Bf sera in 103 patients with epilepsy, and the results were compared with a control group of unrelated healthy individuals of 76 for C4 and 220 for Bf. An increase in gene frequency of Bf F in the paties with epilepsy (0.1942) was found in comparison with that in the normol controls (0.1159). The relative risk value (RR) and etiological fraction (EF)for epilepsy in person who carry the Bf F gene were 1.8379 and 8.85?10~(=2) respectively. About the C4, there is no difference between patients and normal controls.
Résumé
Allotypes and complotypes of Bf, C4A and C4B in 42 families with myasthenia gravis (MG) were determined by means of high voltage agarose gel elect rophoresis of EDTA plasma and subsequent immunofixation, in an attempt to determine whether specific allotypes and/or complotypes were associated with this disease. From the family-data, 108 complotypes were obtained, which could be classified into 28 kinds of complotypes. We found that there are positive linkage disequilibra in S42, S31 and S40,and negative linkage disequilibra in S32 and S41. Compared of these results with those of normal controls, the frequency of S42 is significantly increased in MG(P 0.05).Furhter analysis showed that the MG associated S42 may result from that C4A4 is strongly associated with MG, while C4A4 is in linkage disequilibrium with Bf S and C4A2. Preliminary association mechanism between C4A4 and MG was analysed and discused.