Application of TBL teaching method based on digital slices in the teaching of pathology of oral mucosal disease / 中华医学教育探索杂志

Objective:To construct a teaching model of pathology of oral mucosal disease through team-based learning (TBL) based on digital slices combined with mind mapping, and to explore its teaching effect.Methods:A total of 64 oral medical five-year undergraduates from Batch 2017 who took oral histology and pathology were randomized into 2 groups in average, experimental group and control group. Both groups were asked to do self-study on line before class. Students in the experimental group were taught by TBL method. While students in the control group were taught by the traditional methods. Effects of the different teaching methods were evaluated by comparing the scores of two groups. Besides, questionnaires on teaching effect were distributed to evaluate students' satisfaction with TBL method, with Likert 5 as the guideline for scoring. SPSS 2016 was used to analyze the data.Results:The experimental group achieved better teaching results than the control group did, and the students were more satisfied with TBL method.Conclusion:It can be concluded from the study that, the teaching mode of TBL method combined with mind mapping can cause better teaching effects and improve the students' clinical thinking ability and teamwork ability, which is worthy of wide application.

EB virus-positive T/NK lymphoproliferative diseases: an analysis of 156 patients / 中华病理学杂志

Objective@#To investigate the clinicopathological features of EBV-positive T/NK cell lymphoproliferative diseases (EBV+ T/NK-LPD).@*Methods@#The clinical characteristics of 156 cases of EBV+ T/NK-LPD were collected from August 2002 to March 2015 at Beijing Friendship Hospital, Capital Medical University. Immunohistochemical staining, EBER in situ hybridization and clonal analysis of TCR gene were performed. All patients were followed up.@*Results@#There were 106 male and 50 female patients; patients′ age ranged from 1 to 75 years (median 20 years). The course of the diseases before diagnosis ranged from 2 to 540 months (median 20 months). Fever was noted in 122 patients (78.2%), 108 patients had lymphadenopathy (69.2%), and 75 patients had hepatosplenomegaly (48.1%). Thirty-three cases were grade 1, 68 cases were grade 2, and 55 cases were grade 3. TCR gene arrangement analysis was performed in 45 cases, and 33 cases (73.3%) showed clonal rearrangement. The follow-up period ranged from 1-134 months, and 44 patients (28.2%) died. There was a trend of increased death rate associated with increasing grade (P>0.05).@*Conclusions@#There are many types of EBV+ T/NK-LPD, and they can be classified as systemic, relatively localized and localized. The prognosis should be based on a comprehensive analysis of pathology and clinical data. There is no significant correlation between morphological grade and mortality. An important goal of therapy is to prevent serious complications.

Curative effect of nursing intervention in the treatment of children with bronchial pneumonia and its effects on pulmonary function / 中国基层医药

Objective To observe the curative effect of nursing intervention in the treatment of children with bronchial pneumonia and its effects on pulmonary function.Methods 100 children with bronchial pneumonia were selected as the research subjects,they were randomly divided into control group (50 cases) and observation group (50 cases) according to the digital table.The control group received routine nursing,the observation group received high quality nursing.The pulmonary function indicators (FVC,FEV1,PEF),the adverse reactions and the clinical curative effect were observed in the two groups after treatment.Results After treatment,the FVC,FEV1,PEF of the observation group were (3.96 ± 0.80) L,(3.15 ± 0.80) L,(6.32 ± 2.01) L/s,respectively,which were better than those of the control group [(2.85 ±0.91)L,(2.43 ±0.63)L,(4.90 ± 1.35)L/s] (t =6.478,P=0.000;t =5.000,P =0.000;t =4.147,P =0.000).The incidence rate of adverse reactions of the observation group was 14.00％ (7/50),which was lower than 38.00％ (19/50) of the control group (x2 =7.484,P =0.006).The total effective rate of the observation group was 98.00％ (49/50),which was higher than 80.00％ (40/50) of the control group (x2 =8.274,P =0.004).Conclusion To give children symptomatic treatment of infantile bronchial pneumonia at the same time,take the high quality nursing intervention can effectively improve lung function in children,reduce the incidence of complications,it is conducive to prognosis and can improve the therapeutic efficacy.

The effects of PKC activation on the epithelial-mesenchymal transition of oral squamous cell cancer cells / 实用口腔医学杂志

Objective:To study the effects of activating protein kinase C (PKC)on oral squamous-cell cancer(OSCC) cell epithelial-mesenchymal transition(EMT).Methods:Plasmid pCMV6-AC-GFP-P120ctn was used to transfect HN12 cells to make P120-catenin (120ctn) overexpress and thereafter PKC activator PMA (phorbol 12-myristate 13-acetate) was added to the culture.Real-time fluorescent quantitative PCR and Western blot were adopted to test mRNA and protein expression of PKC,P120ctn,E-cadherin(E-cad),N-cadherin(N-cad) and Vimentin(Vim).Transwell cell invasion and cell migration assay were used to test the invasion and migration capacity before and after the activation.Results:When PKC was activated by PMA,the expression of P120ctn and E-cad were decreased,the cell morphology changed,nRNA and protein expression of mesenchymal marker protein N-cad and Vim increased significantly.Meanwhile,the migration and invasion capacity of the tumor cells increased significantly(P ＜ 0.05).Conclusion:In OSCC cells,PKC may be involved in promoting EMT and the metastasis and invasion by adjusting P120ctn/E-cad expression and cell adhesion.

Protective effect of cinnamic aldehyde on hormone-induced osteoclasts differentiation and its molecular mechanisms / 中国药理学通报

Aim To investigate the protective effect of cinnamic aldehyde ( CA ) on hormone-induced osteo-clasts proliferation and bone resorption in vitro and its molecular mechanisms. Methods RAW264. 7 cells induced into osteoclast were treated with RANKL and M-CSF and then were divided into control group, dexa-methasone ( DEX ) group and different doses of CA (11. 6, 23. 2, 46. 4 μg·L-1 ) groups. OCs were ob-served after tartrate resistant acid phosphatase( TRAP) staining. The cell proliferation was determined by MTT assay at different time points. The expression levels of TRACP5 b in cell cultured supernatants were measured by ELISA. RT-PCR technique was applied to examine the transcriptional levels of RANK and NFATc1 . Re-sults In MTT assay, the proliferation of osteoclasts stimulated by dexamethasone was promoted seriously compared with negative control group ( P < 0. 05 ) . Meanwhile, DEX could strengthen the content of TRACP5 b and up-regulate the expressions of RANK and NFATc1 mRNA. After administration of CA, the proliferation was inhibited, while the enhanced expres-sion of TRAP5b was reversed,and the over-expressions of RANK and NFATc1 mRNA were obviously down-regulated in a time-and-dose-dependent manner ( P <0. 05 ) . Conclusion The results suggest that CA in-hibits proliferation and bone resorption of osteoclast in-duced by DEX, which may be mediated by down-regu-lation of RANK and NFATc1 mRNA.

Characteristics of immunological reconstitution of T-cell subsets after irradiation and immunoregulatory activities of Chinese medicinal herb cornus / 中国免疫学杂志

Objective:To explore the characteristics of immunological reconstitution of T-cell subsets and the role of Cornus,a Chinese medicinal herb on T lymphocytes in mice after irradiation (IR). Methods:Irradiated model mice were exposed to a single dose of X-ray radiation (2. 6 Gy) with or without Cornus treatments. Bloodroutine was examined before or after irradiation. CD3+,CD4+, CD8+ T cells and Th1, Tc1, Th2, Th17, Treg from spleen or peripheral blood were determined by flow cytometry. Results: Total lymphocytes and CD3+T cells including CD4+T and CD8+T cells,were significantly reduced 3 days after irradiation (P<0. 05). The re-constitution of CD3+ T cells ( especially CD8+T cells ) started from 5 days post irradiation, CD4+T cells increased 8 days after irradiation. However,the production of IFN-γ by Th1 or Tc1 cells were evidently decreased compared with control group even 8 days post irradiation (P<0. 05). In contrast to Th1,Th2/Th17/Treg were observably increased in irradiation group (P<0. 05). But the percentage of Th1 was obviously increased, and Th2, Th17, Treg were markedly decreased in cornus treated mice compared with irradiated mice (P<0. 05). Conclusion: The immunological reconstitution of T lymphocytes started from 5 days after total irradiation with rapid recovery of CD8+ T cells but not CD4+T cells. Cornus effectively improved the imbalance of T cell subsets by promoting the proliferation of Th1 and suppressing Th17 and Treg.

Comparison of the effect of different ways of using tirofiban in patients with acute ST segment elevation myo-cardial infarction undergoing percutaneous coronary intervention / 中国基层医药

Objective To study tirofiban intravenous injection,coronary artery injection of the two different methods in acute ST segment elevation myocardial infarction,the application of emergency PCI.Methods Patients underwent emergency PCI with acute ST segment elevation myocardial infarction as the research subjects,a total of 108 cases,the patients were randomly divided into the observation group and control group,54 patients in each group. In the observation group,the first dose of tirofiban was injected into the coronary artery.The control group was treated by intravenous injection.The results of the two groups were compared.Results Before treatment,TIMI level 2 and level 3 ratio,initial corrected TIMI frame count of the observation group were significantly lower than the control group (χ2 /t =4.32,4.59,5.25,all P 0.05).Conclusion Compared with intravenous injection,tirofiban in the treatment of acute ST segment elevation myocardial infarction by intracoronary injection can improve the level of myocardial perfusion after PCI operation,promote the recovery of left ventricular function,and has high security.

The relationship between T lymphocyte subsets and hepatitis B reactivation after chemotherapy / 中国基层医药

Objective To explore the changes ofT lymphocyte subsets CD3+,CD4+,CD8+ in hepatitis B reactivation after chemotherapy.Methods Flow cytometry was used to detect T lymphocyte subsets of 32 patients with hepatitis B reactivation before and after chemotherapy.In addition,25 patients without hepatitis B reactivition were chosen as control group.Results After chemotherapy,the percentage of CD3+ and CD4+ T lymphocytes and the ratio of CD4+/CDs+ were significantly reduced than those before chemotherapy (P ＜ 0.05).However,the percentage of CD8+ T lymphocytes was not significantly changed(P ＞ 0.05).Conclusion The chemotherapy can change the number and ratio of T lymphocyte subsets,the inhibition of the cellular immune function is one of the reason of hepatitis B virus reactivation.

Significance of CD123 in histiocytic necrotizing lymphadenitis / 中华病理学杂志

<p><b>OBJECTIVE</b>To investigate the mode of presentation, cytologic features of the plasmacytoid dendritic cells (pDC), and the expression of CD123 and its significance in Kikuchi's disease.</p><p><b>METHODS</b>CD123 expression was evaluated by EliVision immunohistochemical staining in formalin-fixed and paraffin-embedded tissues from 30 cases of Kikuchi's disease, 5 cases of T cell lymphoma, 10 cases of reactive lymphoid hyperplasia and 10 cases of chronic tonsillitis.</p><p><b>RESULTS</b>Clusters of CD123 positive PDC were observed in Kikuchi's disease (28 of 30 cases, 93.3%) and the staining intensity was more prominent in the PDC at the periphery of the lesion and around the high endothelial venule-like vessels. CD123 showed three staining patterns: membranous (10 cases, 33.3%), cytoplasmic (10 cases, 33.3%), and membranous and cytoplasmic (8 cases, 26.7%). In the control group, CD123 showed cytoplasmic staining in reactive hyperplasia and chronic tonsillitis. Regarding the staining intensity, 12 of 28 cases (42.9%) were 3+ for CD123, 8 of 28 cases (28.6%) were 2+, and 8 of 28 cases (28.6%) were 1+. In contrast, PDC clusters with 1+ staining intensity were observed in 1 of 10 cases of reactive lymphoid hyperplasia; 2 of 10 chronic tonsillitis diseases; and much less in T cell lymphoma.</p><p><b>CONCLUSIONS</b>Large cluster of PDC is detected in both proliferative and necrotizing types of Kikuchi's disease, making this a useful adjunctive diagnostic marker.</p>

Study on the relationship between blood T-lymphocyte subsets level and hepatitis B virus reactivation / 中国基层医药

Objective To investigate the relationship between hepatitis B virus reactivation and the level of blood T-lymphocyte subsets.Methods T lymphocyte subsets ( CD3,CD4,CDs ),CD4/CDs level of 40 patients with chronic carriers of hepatitis B virus and 35 patients with hepatitis B virus re-activation were detected by Flow cytometry(Epics XL).Results The hepatitis B virus reactivation in patients with CD3,CD4 and CD4/CDs levels were significantly lower than the chronic hepatitis B virus carriers,The difference between the two groups patients was significant[(71.31 ±5.20)％ vs (68.57 ±6.10)％,(37.82 ±4.90)％ vs (32.12 ±5.93)％,(1.37 ±0.28) vs (1.18 ±0.43 ),all P ＜ 0.05].Conclusion The levels of CD3,CD4 and CD4/CD8 ratio were significantly declined,it may be the one important reason of hepatitis B virus reactivation.

A clinicopathological study of 10 cases of neoplastic cell rich Hodgkin's lymphoma / 白血病·淋巴瘤

Objective To clarify clinical and morphological features and immunophenotype and Epstain-Barr virus infection of neoplastic cell rich Hodgkin's lymphoma (NCRHL)and to further improve our knowledge and pathological diagnosis for NCRHL. Methods 10 cases of NCRHL were analyzed for clinical features, morphology, immunophenotype, Epstein-Barr virus infection using routine eosin and haematoxylin stain, immunohistochemistry, Epstain-Barr virus encoded small RNA (EBER) in situ hybridization and combining clinical data. Results (1)NCRHL were more common in young people. The median age of the patients was 25.5 years old. The ratio of male to female was 1:2.3. Superficial lymph nodes were most frequently involved. Masses of mediastinum were seen commonly. Clinical manifestation of the patients included B symptom (6 cases), pruitus (5 cases) and anemia (1 case). (2)Architecture of lymph nodes were effected. Necrosis was seen in some cases. There were more tumor cells in NCRHL than that in the classical Hodgkin's lymphoma. The tumor cells were distributed in piece or patch or diffuse. The morphology of neoplastic cells was wore variable including Hodgkin-like cells, lacunar cell-like, mummy cell-like and anaplastic large cell-like, singular nucleated cells, and multinucleated giant cell-like cells. Numerous neutrophils and eosinophils were present in a few cases. Focal sheet, necrosis granulomatosis-like and diffuse growth pattern were found in NCRHL. (3)All of the cases were positive for CD30 and PAX-5.2/10 (20%) cases were CD15 positive. LCA, CD20 and CD3 were negative. (4)EBER was not detected in all 6 tested cases. (5)Follow up data was obtained in 8/10 cases, in which one patient was dead, one case relapsed in half a year,and the other 6 cases reached complete regression. Conclusion NRCHL is characterized mainly by neoplastic cell rich morphologically and focal sheet, necrosis granulomatosis-like and diffuse growth pattern.EBER was not detected in this tumor. Some cases have aggressive clinic process with a unfavourable prognosis. New treatment regimen should be explored.

Clinical analysis on the level of serum ferritin in patients with severe hepatitis B / 中国基层医药

Objective To study the correlation between the level of serum ferritin(SF) and the degree of liver damagement with severe hepatitis B,and the clinical significance of SF changes in judging the prognosis.Methods The level of SF was detected by radioimmunoassay(RIA) from 62 cases with severe hepatitis B,the level of SF from acute hepatitis B,chronic hepatitis B,liver cirrhosis and normal men were served as contrast study.Results The level of SF from severe hepatitis B was significantly higher than those from acute hepatitis B,chronic hepatitis B,liver cirrhosis and normal men;the level of SF was positively correlated with total bilirubin(TB),prothrombin time(PT),total bile acid(TBA) and negatively with Alb,ALT,AST,CHE.The levels of SF of those who died were significantly higher than those of suvivals;the level of SF decreased as the disease controlled and increased as the disease deterio-rated.Conclusions There is a parallel correlationship between the level of SF and the degree of liver damagement with severe hepatitis B,the severer the hepatocyte damage was,the higher the ferritin was.It is helpful to judge the degree of damagement and prognosis of severe hepatitis by detecting the level of serum ferritin.

The analysis of pregnancy with virus hepatitis / 中国基层医药

Objective To analyse the pathogeny of pregnancy with virus hepatitis,the change of index of hepatic function and the clinical pathogenetic characteristic,and to explore the relationship between pregnancy and hepatitis.Methods The clinical datas of 96 pregnant patients with virus hepatitis were retrospectively analyzed,and compared with non-pregnancy during the reprodective age in the corresponding period.The pathogeny,index of hepatic function and clinical pathogenetic characteristic were compared.Results Virus hepatitis in the pregnancy were mostly type B hepatits,the morbility of hepatitis increased gradually along with the progress of pregnant weeks,the prothrombin time(PT) significantly prolonged in pregnancy with virus hepatitis,and the albumin decreased significantly,the complications of hepatocerebral disease and hepatorenal syndrome were more than non-pregnancy with virus hepatits,the incidence rate of serious hepatiti was higher than non-pregnancy with virus hepatitis(P