Résumé
Background@#Maedi/Visna virus (MVV) is a contagious viral pathogen that causes considerable economic losses to the sheep industry worldwide. @*Objectives@#In China, MVV has been detected in several regions, but its molecular characteristics and genetic variations were not thoroughly investigated. @*Methods@#Therefore, in this study, we conducted next-generation sequencing on an MVV strain obtained from northwest China to reveal its genetic evolution via phylogenetic analysis. @*Results@#A MVV strain obtained from Inner Mongolia (NM) of China was identified. Sequence analysis indicated that its whole-genome length is 9193 bp. Homology comparison of nucleotides between the NM strain and reference strains showed that the sequence homology of gag and env were 77.1%–86.8% and 67.7%–75.5%, respectively. Phylogenetic analysis revealed that the NM strain was closely related to the reference strains isolated from America, which belong to the A2 type. Notably, there were 5 amino acid insertions in variable region 4 and a highly variable motif at the C-terminal of the surface glycoprotein (SU5). @*Conclusions@#The present study is the first to show the whole-genome sequence of an MVV obtained from China. The detailed analyses provide essential information for understanding the genetic characteristics of MVV, and the results enrich the MVV library.
Résumé
Objective Combining the concept of double loop learning in enterprise management with individualized nursing practice,the application value of this method in nursing care of patients with early onset severe preeclampsia(ESPE)was analyzed. Methods Patients with ESPE were selected from January 2014 to December 2016, which were divided into observation group and control group by random digits table method. The patients in observation group were given individualized nursing care with the idea of double loop learning,while the patients in control group received routine nursing care. The hospital admission, maternal pregnancy outcome and nursing satisfaction of the patients were compared between two groups. Results The observation group included 96 cases and the control group included 101 cases. There was no significant difference between two groups in age, gestational age, systolic blood pressure, diastolic blood pressure, and 24 hours urine protein(P>0.05).There were 87 cases of cesarean section in the observation group,while 94 cases in the control group, and there was no significant difference(P>0.05). The gestational weeks,the incidence of neonatal asphyxia,the nursing satisfaction was(35.5±2.7)weeks,5.2%(5/96), 90.6%(87/96)in the observation group and(34.1±2.0)weeks, 15.8%(16/101),72.3%(73/101)in the control group, the difference was statistically significant(t=4.149, χ2=5.843, 10.862, P<0.05 or 0.01). Conclusions In the ESPE nursing management, the individualized nursing and double loop learning can improve the child pregnancy outcomes and nursing satisfaction.