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Objective To investigate the efficacy of Kinesio taping combined with warm needling on knee osteoarthritis (KOA) and its effect on serum levels of immune globulin(Ig)A, IgG, IgM. Methods Sixty-two KOA patients who met the inclusion criteria were divided into 2 groups by random number table (31 cases in each group). The control group was treated with routine thrapy. Based on the control group, the observation group was treated with Kinesio taping combined with warm needling. After 6 weeks treatment, scores of visual analogue scale (VAS) was used to evaluate knee pain, Japanese orthopaedic association (JOA) scale was used to evaluate knee function, and Lequesne index was used to evaluate disease severity. The score of TCM clinical symptoms was conducted according to the KOA symptom grading quantification table, and the quality of life was evaluated according to the Chinese version of the MOS item short from health survey (SF-36). The Immunoglobulin(Ig)A, IgG and IgM levels were detected by ELISA method. Results The total effective rate in the observation group was 93.5%, and the control group was 71.0% (28/31). The difference was statistically significant between two groups (χ2=5.415, P=0.020).The total effective rate of clinical symptoms in traditional Chinese medicine in the observation groups as 90.3% (28/31) was higher than the control group as 64.5% (20/31), and the difference was statistically significant (χ2=5.905, P=0.015). After treatment, the serum level of IgA (2.91 ± 0.35 ng/ml vs. 4.50 ± 0.50 ng/ml, t=7.621), IgG (11.17 ± 1.31 pmol/L vs. 15.83 ± 1.94 pmol/L, t=13.523), IgM (3.14 ±0.44 pmol/L vs. 5.44 ±0.62 pmol/L, t=8.441) were significantly lower than control group (P<0.01). After 6 weeks’ treatment, tthe scores of VAS, Lequesne index, and clinical symptom of traditional Chinese medicin of the observation group were significant lower than those of the control group (t=7.765 and 9.903 respectively, all Ps<0.01), but the scores of JOA and the SF-36 were significantly higher than those of the control group (t=18.441, P<0.01). After 6 weeks’ treatment, the scores of pain in bed at night, morning stiffness, walking pain, pain from sitting up, maximum walking distance, daily activities, walking up stairs, walking down stairs, bending knee joint score in the observation group were significantly lower than those in the control group (t=8.933, 9.112, 9.225, 8.809, 9.240, 8.910, 7.903, 8.033, 7.031, respectively, all Ps<0.01), but the scores of physical pain, physiological function, physiological function, vitality, social function, emotional function, mental health in the observation group were significantly higher than those in the control group (t=12.450, 13.446, 13.779, 9.003, 13.331, 12.440, 15.309, all Ps<0.01). Conclusions The kinesio taping combined with warm needling can improve symptoms of traditional Chinese medicine and reduce pain of KOAand increase quality of life with the regulation of IgA, IgG, IgM.
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Objective@#To evaluate the feasibility of intravoxel incoherent motion diffusion-weighted magnetic resonance imaging (IVIM-DWI MRI) in the evaluation of tumor vascular normalization in a mouse model of colorectal cancer induced by recombinant human endostatin (rhES).@*Methods@#The CT26 colorectal cancer xenograft model of BALB/c mice were established and divided into rhES group and control group, with 20 mice in each group. The mice of rhES group were intravenously injected with rhES 5 mg·kg-1·d-1 once daily for 12 days, while the mice of the control group were intravenously injected with the same volume of 0.9% saline. 5 mice of rhES group and control group were randomly selected to perform IVIM-DWI MRI as following times: before treatment and four, eight, twelve days after treatment. The parameters of IVIM-DWI were recorded, including true diffusion coefficient(D), pseudo-diffusion coefficient (D*) and perfusion fraction (f). Meanwhile, microvessel density (MVD), pericyte coverage and tumor perfusion in tumor tissues were detected by immunofluorescence, respectively.@*Results@#The tumor volumes of control group and rhES group before treatment were (154.42±24.65) mm3 and (174.24±28.27)mm3, respectively, without statistically significant difference (P=0.440). From day 2 to day 12 after treatment, the tumor volume of rhES group was significantly smaller than that of control group (all P<0.05). There were no statistical significances of D value between the rhES group and control group before and after treatment (all P>0.05). The D* values of the rhES group were (10.940±2.834)×10-3mm2/s and (12.940±2.801)×10-3mm2/s in day 4 and 8 after treatment respectively, significantly higher than (6.980±1.554)×10-3mm2/s and (7.898±1.603)×10-3mm2/s of control group (P<0.05). Moreover, compared with control group, the D* value of rhES group was significantly lower in day 12 (6.848±1.460)×10-3mm2/s vs (9.950±2.596)×10-3mm2/s, (P<0.05). The f value of rhES group in day 8 was (0.226±0.021)%, significantly higher than (0.178±0.016)% of control group (P<0.01). The MVD of rhES group was significantly lower than that of control group (P<0.05), while the pericyte coverage and tumor perfusion of rhES group were significantly higher than those of control group in day 4 and 8 after treatment (all P<0.05). In addition, we found D* value of IVIM-DWI in rhES group was significantly related with MVD, pericyte coverage and tumor perfusion (r=-0.354, r=0.555, r=0.559, all P<0.05). Meanwhile, the f value in rhES group was also significantly related with MVD, pericyte coverage and tumor perfusion (r=-0.391, r=0.538, r=0.315, all P<0.05).@*Conclusions@#IVIM-DWI MRI can effectively evaluate the vascular normalization in rhES-induced CT26 colorectal tumor.The parameters D* and f are closely related to intratumorally microvessel density, pericyte coverage and perfusion, which can effectively monitor the occurrence of tumor vascular normalization time.
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Objective@#To investigate the efficacy of Kinesio taping combined with warm needling on knee osteoarthritis (KOA) and its effect on serum levels of immune globulin(Ig)A, IgG, IgM.@*Methods@#Sixty-two KOA patients who met the inclusion criteria were divided into 2 groups by random number table (31 cases in each group). The control group was treated with routine thrapy. Based on the control group, the observation group was treated with Kinesio taping combined with warm needling. After 6 weeks treatment, scores of visual analogue scale (VAS) was used to evaluate knee pain, Japanese orthopaedic association (JOA) scale was used to evaluate knee function, and Lequesne index was used to evaluate disease severity. The score of TCM clinical symptoms was conducted according to the KOA symptom grading quantification table, and the quality of life was evaluated according to the Chinese version of the MOS item short from health survey (SF-36). The Immunoglobulin(Ig)A, IgG and IgM levels were detected by ELISA method.@*Results@#The total effective rate in the observation group was 93.5%, and the control group was 71.0% (28/31). The difference was statistically significant between two groups (χ2=5.415, P=0.020).The total effective rate of clinical symptoms in traditional Chinese medicine in the observation groups as 90.3% (28/31) was higher than the control group as 64.5% (20/31), and the difference was statistically significant (χ2=5.905, P=0.015). After treatment, the serum level of IgA (2.91 ± 0.35 ng/ml vs. 4.50 ± 0.50 ng/ml, t=7.621), IgG (11.17 ± 1.31 pmol/L vs. 15.83 ± 1.94 pmol/L, t=13.523), IgM (3.14 ± 0.44 pmol/L vs. 5.44 ± 0.62 pmol/L, t=8.441) were significantly lower than control group (P<0.01). After 6 weeks’ treatment, tthe scores of VAS, Lequesne index, and clinical symptom of traditional Chinese medicin of the observation group were significant lower than those of the control group (t=7.765 and 9.903 respectively, all Ps<0.01), but the scores of JOA and the SF-36 were significantly higher than those of the control group (t=18.441, P<0.01). After 6 weeks’ treatment, the scores of pain in bed at night, morning stiffness, walking pain, pain from sitting up, maximum walking distance, daily activities, walking up stairs, walking down stairs, bending knee joint score in the observation group were significantly lower than those in the control group (t=8.933, 9.112, 9.225, 8.809, 9.240, 8.910, 7.903, 8.033, 7.031, respectively, all Ps<0.01), but the scores of physical pain, physiological function, physiological function, vitality, social function, emotional function, mental health in the observation group were significantly higher than those in the control group (t=12.450, 13.446, 13.779, 9.003, 13.331, 12.440, 15.309, all Ps<0.01).@*Conclusions@#The kinesio taping combined with warm needling can improve symptoms of traditional Chinese medicine and reduce pain of KOAand increase quality of life with the regulation of IgA, IgG, IgM.
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Internal medicine nursing is a very important learning course for nursing students. It is divided into two semesters in our university. Because the knowledge of the course is more complex and in-volves a wide range, many students have a poor learning effect. In order to improve the teaching effect of the course, the students leave the time to build the "summary", "extension" and "preview" combined guidance method. "Summary" is on the learning content of the summary, "extension" is the effective extension of learning content to the students, "preview" is the key content of the pre term learning habit. Then, accord-ing to the final score of medical nursing in the last semester, we can flexibly design the teaching link. For students under 60 points, holiday guidance is mainly "summary", for the students between 60 points to 85 points, holiday guidance is combined with "summary" "extension" and "preview", for students above 85 points, holiday guidance is the main "extension". Thus, the students' study and holiday time are integrated into a whole to improve their overall learning efficiency.
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Objective To investigate the method and effect of endovascular treatment for ilio-femoral venous post-thrombotic syndrome.Methods We retrospectively analyzed the clinical data of 47 patients,who underwent endovascular treatment for ilio-femoral venous post-thrombotic syndrome from January 2010 to December 2013.Patients received transjugular iliofemoral venous thrombectomy,percutaneous angioplasty (PTA) and/or endovascular stent placement after inferior vena cava filter was implanted.Results All patients were successfully treated with angioplasty and stent placement.The ilio-femoral venous were patent after the placement of the stent.Chronic ulcer in 7 patients healed within one month.The patency rate of the affected limb at 6 months was 88.9％,occlusion rate was 11.1％.No reblocked cases were found during venous ultrasound follow-up.After treatment,the circumference difference of low limb between affected and normal limb decreased from (2.46 ± 0.98) cm to (0.70 ± 0.19) cm (P ＜0.05) at the level of 15 cm above knee,and from (3.28 ±0.85) cm to (0.83 ±0.26) cm (P＜0.05) at the level of 15 cm below knee.Villalta score decreased from (12.0 ± 1.9) to (6.9 ±2.2,P ＜ 0.05) and symptoms were significantly improved.Conclusions Ilio-femoral venous stenting provides a safe,effective and minimal invasive approach for the treatment of post-thrombotic syndrome.Acceptable patency rates can be expected through short-term follow-up.
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AIM:To investigate whether gold nanoparticles (GNPs) reverses adriamycin (ADM), resistance of human hepatocellular carcinoma drug-resistant cell line HepG2/ADM and to explore the potential mechanism.METH-ODS:The sensitivities of HepG2 cells and HepG2/ADM cells to ADM were tested by MTT assay before and after GNPs pretreatment.The apoptotic rate was examined by flow cytometry.The concentration of ADM in HepG2/ADM or HepG2 cells was determined by ultraviolet-visible spectrophotometer .The content of glutathione ( GSH ) in HepG2/ADM or HepG2 cells by DTNB method.RESULTS:The half maximal inhibitory concentrations ( IC50 ) of ADM for HepG2/ADM cells were(29.46 ±1.73) mg/L and (15.18 ±0.85) mg/L before and after GNPs pretreatment,respectively.The IC50 of ADM for HepG2 cells was (9.16 ±2.03) mg/L before pretreatment.The apoptotic rate in GNPs+ADM group was higher than that in ADM group ( P reduce the content of GSH and increase the concentration of ADM in HepG2/ADM cells.
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Objective To discuss and analyze the issue in the catheter directed thrombolysis(CDT) treatment of acute lower extremity deep venous thrombosis.Methods The clinical data of 182 cases of acute lower extremity deep venous thrombosis from January 2011 to January 2013 which were treated by CDT were retrospectively analyzed,and the issues in these treatment were discussed and analyzed.Results The effect were classified into three degrees.Thrombosis clearance exceed 95 percent belonged to the first degree,among 50 percent and 95 percent belonged to the second degree,and below 50 percent belonged to the third degree.In this study,the respective number of each degree were 93/68/21.Sixty-one cases combined iliac venous compression syndrome were treated by stent.Twenty-two cases were treated with inferior vena cava filter.All the patients were followed up 10 months,148 cases of them maintained patency.Conclusions The effect of CDT treatment to acute lower extremity deep venous thrombosis is remarkable,meanwhile the patency is improved and the deep vein thrombosis were decreased through this method.But the issues of access,filter and iliac venous compression syndrome should also be considered.
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Thyroid hormone is an important hormone for regulating cell differentiation,growth and metabolism.Many studies have shown that thyroid hormone can induce turmor angiogenesis after binding with the integrin ανβ3 receptor at cell surface.Tetraiodothyroacetic acid is the analogue of thyroid hormone,and can inhibit tumor angiogenesis by inhibiting the binding of thyroid hormone with integrin ανβ3.Studying the role of thyroid hormone in promoting tumor angiogenesis may provide a new approach for molecular targeted therapy of tumor.