The effect of nitric oxide and total antioxide capacity on the germ cell apoptosis in the rat cryptorchid / 中华男科学杂志

<p><b>OBJECTIVES</b>To study the effect of nitric oxide (NO) and total antioxide capacity (T-AOC) on the germ cell apoptosis in the cryptorchidism rat.</p><p><b>METHODS</b>Immature rats (22 day-olds Sprague Dawley) were selected to make the model of unilateral cryptorchid. Germ cell apoptosis was examined by terminal-deoxynucleotidyl transferase mediated d-UTP nick end labeling (TUNEL). Biochemical parameters (NO, T-AOC) were evaluated with spectrophotometric determination.</p><p><b>RESULTS</b>At the seventh day after the operation, as compared to the normal side testis, the number of apoptotic germ cells increased significantly in the undescended testis (P < 0.01), but the testes weight decreased predominantly; the NO level in the undescended testis was significantly higher than that in the normal side testis, and the T-AOC level was predominantly lower (P < 0.01).</p><p><b>CONCLUSIONS</b>Experimentally-induced cryptorchidism can lead to the increase of grem cell apoptosis in rat. The results is closely related to the increase of No level and the decrease of T-AOC.</p>

Investigation of the mechanism of genitofemoral nerve in the contralateral testes damage of unilateral cryptorchid rats / 中华男科学杂志

<p><b>OBJECTIVES</b>To investigate the mechanism of genitofemoral nerve in the contralateral testes damage of unilateral cryptorchid rats.</p><p><b>METHODS</b>After unilateral cryptorchid rat model (21-day) was made and the ipsilateral genitofemoral nerve was divided, the germ cells apoptosis and the concentrations of lactate of contralateral testes were detected and compared after 120 days.</p><p><b>RESULTS</b>After the ipsilateral genitofemoral nerve was divided, the germ cells apoptosis and the lactate levels of the contralateral testes were all decreased from (17.28 +/- 1.36)% to (5.76 +/- 0.76)% and (2.19 +/- 0.24) mmol/L to (1.70 +/- 0.31) mmol/L, respectively.</p><p><b>CONCLUSIONS</b>The damage pathway of the contralateral testes of unilateral cryptorchidism probably related to the apoptosis of the germ cells which caused by the reflex decrease of the contralateral testicular blood flow via the transmissions of genitofemoral nerve.</p>