An Improved Method for Electroencephalographic Detection of Epileptic Discharge / 中国医学科学院学报

Objective To investigate the role of OpenBCI module in the electroencephalographic (EEG) detection of epileptic discharge.Methods C57BL/6J mice aged 8-12 weeks were divided into two groups:the sham-operated group and kainic acid-induced epileptic group. Spontaneous seizures were monitored continuously for 3 weeks either by EEG or by OpenBCI.Results Up to 8 mice could be simultaneously monitored by OpenBCI. Meanwhile,the module accurately recorded the resting discharge,EEG spikes,and seizures.Conclusion Compared with the conventional brain function monitoring system,the OpenBCI module has lower cost and data occupancy and thus may be applied in clinical settings.

Comparison of β-Amyloid Plaque Labeling Methods: Antibody Staining, Gallyas Silver Staining, and Thioflavin-S Staining / 中国医学科学杂志(英文版)

Objective To evaluate senile plaque formation and compare the sensitivity of three different β-amyloid (Aβ) labeling methods (antibody staining, Gallyas silver staining, and thioflavin-S staining) to detect Aβ deposition.Methods APPswe/PSEN1dE9 transgenic mice (APP/PS1) of different ages were used to examine spatiotemporal changes in Aβ plaque deposition. Antibody staining, Gallyas silver staining, and thioflavin-S staining were used to detect Aβ plaque deposition in the same brain region of adjacent slices from model mice, and the results were compared.Results With aging, Aβ plaques first appeared in the cortex and then the deposition increased throughout the whole brain. Significantly greater plaque deposition was detected by 6E10 antibody than that analyzed with Gallyas silver staining or thioflavin-S staining (P<0.05). Plaque deposition did not show significant difference between the APP/PS1 mice brains assayed with Gallyas silver staining and ones with thioflavin-S staining (P=0.0033).Conclusions The APP/PS1 mouse model of Alzheimer's disease could mimick the progress of Aβ plaques occurred in patients with Alzheimer's disease. Antibody detection of Aβ deposition may be more sensitive than chemical staining methods.