Résumé
Taking Guangdong Province Traditional Chinese Medical Hospital as an example,the paper introduces the study idea of hospital IT intelligent operation maintenance and monitoring platform,expounds on design and realization of the platform,including general technical architecture,functional architecture and core business process,summarizes related practical experiences,and points out that application of the platform is able to enhance operation maintenance level and user satisfaction significantly.
Résumé
<p><b>OBJECTIVE</b>To explore practical protocols for cloning bone marrow-derived hepatic stem cells in vitro.</p><p><b>METHODS</b>The cell fraction rich in CD117(+) cells and CD184(+) cells was separated from fresh bone marrow by density gradient centrifugation and cultured for 0, 7 and 14 days in high-glucose DMEM supplemented with or without 10% autologous serum or in serum-free high-glucose DMEM. All the media were supplemented with different concentrations of hepatocyte growth promoting factors (HGPF), thrombopoietin (TPO) and interleukin-3 (IL-3). The quantitative changes of CD117(+) cells and CD184(+) cells were measured by flow cytometry.</p><p><b>RESULTS</b>The optimal effect for cell cloning was achieved with high-glucose DMEM with 10% autologous serum group supplemented with 40 microg/ml HGPF, 50 ng/ml TPO, and 10 ng/ml IL-3. At day 7 of cell culture in this media, the quantity of CD117(+) cells and CD184(+) cells increased by 6.55 and 6.20 folds, and by 11.62 and 20.57 folds at day 14, respectively.</p><p><b>CONCLUSION</b>It is practical for cloning bone marrow-derived hepatic stem cells in high-glucose DMEM with 10% autologous serum supplemented with 40 microg/ml HGPF, 50 ng/ml TPO, and 10 ng/ml IL-3.</p>
Sujets)
Humains , Cellules de la moelle osseuse , Biologie cellulaire , Techniques de culture cellulaire , Clones cellulaires , Facteur de croissance des hépatocytes , Pharmacologie , Hépatocytes , Biologie cellulaire , Physiologie , Foie , Biologie cellulaire , Protéines proto-oncogènes c-kit , Métabolisme , Cellules souches , Biologie cellulaire , Thrombopoïétine , PharmacologieRésumé
<p><b>OBJECTIVE</b>To observe the change in the amount of sialic acids on hepatocellular carcinoma (HCC) cell membrane.</p><p><b>METHODS</b>Surgical specimens of HCC and liver cirrhosis tissues were obtained from 28 patients to prepare carcinoma cell and hepatocyte suspensions by collagenase digestion. For assay of α2, 3 and α2, 6-sialic acids, the cells were suspended in the staining buffer containing either fluorescein isothiocyanate-Maackia amurensis lectin (FITC-MAL) or fluorescein isothiocyanate-Sambucus nigra bark lectin (FITC-SNA) and incubated for 1 h, respectively. Flow cytometric analysis was carried out to measure the mean fluorescence intensity (MFI) on the cell surface.</p><p><b>RESULTS</b>In both FITC-MAL- and FITC-SNA-incubated HCC cells, the MFI on the cell surface was greater than that of the hepatocytes.</p><p><b>CONCLUSION</b>Both of α2, 3 and α2, 6- sialic acids increases significantly on the hepatocyte membrane after the carcinomatous change.</p>