Résumé
<p><b>AIM</b>To investigate the effect of DAPT (gamma-secretase inhibitor) on the growth of human tongue carcinoma cells and to determine the molecular mechanism to enable the potential application of DAPT to the treatment of tongue carcinoma.</p><p><b>METHODOLOGY</b>Human tongue carcinoma Tca8113 cells were cultured with DAPT. Cell growth was determined using Indigotic Reduction method. The cell cycle and apoptosis were analyzed by flow cytometry. Real-time PCR and Immuno-Fluorescence (IF) were employed to determine the intracellular expression levels.</p><p><b>RESULTS</b>DAPT inhibited the growth of human tongue carcinoma Tca8113 cells by inducing G0-G1 cell cycle arrest and apoptosis. The mRNA levels of Hairy/Enhancer of Split-1 (Hes-1), a target of Notch activation, were reduced by DAPT in a dose-dependent manner. Coincident with this observation, DAPT induced a dose-dependent promotion of constitutive Caspase-3 in Tca8113 cells.</p><p><b>CONCLUSION</b>DAPT may have a therapeutic value for human tongue carcinoma. Moreover, the effects of DAPT in tumor inhibition may arise partly via the modulation of Notch-1 and Caspase-3.</p>
Sujets)
Humains , Amyloid precursor protein secretases , Antinéoplasiques , Pharmacologie , Apoptose , Facteurs de transcription à motif basique hélice-boucle-hélice , Carcinomes , Anatomopathologie , Caspase-3 , Lignée cellulaire tumorale , Membrane cellulaire , Noyau de la cellule , Cycline D1 , Dipeptides , Pharmacologie , Relation dose-effet des médicaments , Phase G1 , Protéines à homéodomaine , Récepteur Notch1 , Protéines de répression , Phase G0 , Tumeurs de la langue , Anatomopathologie , Facteur de transcription HES-1Résumé
<p><b>OBJECTIVE</b>To evaluate the influence of different types of posts and cores on the final color of the IPS-Emperss 2 crown.</p><p><b>METHODS</b>Five types of posts and cores (Cerapost with Empress cosmo, Cerapost with composite resin, gilded Ni-Cr alloy, gold alloy and Ni-Cr alloy) were made. The shifts in color of three points of IPS-Empress 2 crown surface (cervical, middle and incisal) with different posts and cores was measured with a spectroradiometer (PR-650).</p><p><b>RESULTS</b>The L* a* b* values of zirconium oxide and gilded Ni-Cr alloy posts and cores with ceramic crown were the highest. The L* a* values of zirconium oxide posts composite cores were higher while the b* values were lower. The L* a* b* values of Ni-Cr alloy were lower than that of gold alloy and were the lowest.</p><p><b>CONCLUSION</b>In combination with IPS-Empress 2 crown, zirconium oxide posts are suitable for routine use in the anterior dentition, and gilded Ni-Cr alloy and gold alloy posts and cores can be recommended for clinical practice. Ni-Cr alloy posts and cores can not be recommended for clinical practice.</p>