EMS mutation of suspension cells and selection of high temperature tolerant mutants from Pinellia ternata / 中国中药杂志

<p><b>OBJECTIVE</b>To determine the optimal concentration and processing time of EMS mutation for suspension cells from Pinellia ternata.</p><p><b>METHOD</b>Under four EMS concentration gradients (0.1% , 0.2%, 0.4%, 0.6%) and three processing time gradients (0.5, 1.0, 2.0 h), the suspension cells of P. ternata were mutagenized.</p><p><b>RESULT AND CONCLUSION</b>The results showed that the survival rate was significantly different under the different concentrations of EMS and the different processing time. In the same processing time, the EMS concentrations were increased, but the suspension cells survival rate decreased gradually. The optimum EMS concentration for the mutagenesis was 0.4% and the best processing time was 1 hour.</p>

Changes of total RNA and mRNA differential expressions in leaves of Pinellia ternata under high temperature stress / 中国中药杂志

<p><b>OBJECTIVE</b>To study the total RNA and mRNA differential expression in leaves of Pinellia ternata under high temperature, provide more information of the molecular mechanism of the sprout tumble.</p><p><b>METHOD</b>The total RNA and mRNA differential expression in leaves of P. ternata at different stress time was analyzed.</p><p><b>RESULT</b>The results showed that the trend of total RNA content was divided into three descending stages and two ascending stages, the total RNA content was the highest at 0, 6 h, but it was the lowest at and 42 h, as well as when the sprout tumbled. The differential display showed that the polymorphism and type of bands of the sample at 6 h were similar to those at 0 h. But the bands numbers at other time were far less than those at 0, 6 h. And there were some different mRNA differential expression bands between the different samples.</p><p><b>CONCLUSION</b>In the process of the sprout tumble caused by high temperature stress, the RNA and mRNA differential expression in leaves of P. ternata changed.</p>