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Objective To compare the clinical features of drug induced hepatitis caused by traditional Chinese medicines (TCM) and western medicines. Methods A total of 181 patients hospitalized with drug in-duced hepatitis between January and December 2015 were enrolled. Among the patients ,75 cases were in TCM group,66 cases in western medicine group and 40 cases in combined group(accepted both TCM and western medi-cine treatment). Liver biopsies were performed and Roussed Uclaf Causality Assessment Method (RUCAM) scores showed that all patients were with scores higher than 3. The data including gender,age,clinical manifesta-tions,physical signs,laboratory tests and image characteristics were analyzed individually. Results There was no significant difference in age,the pattern of liver injury,and the course of liver damage among the three groups (P>0.05). Anorexia was common symptom in all the three groups without any significant difference(P>0.05). Jaundice was most frequently observed in TCM group while fever was most commonly found in western medicine group,which both had significant difference(P0.05). Compared with western medicine group ,the patients in TCM group had a higher level of platelet counts ,serum levels of total bilirubin ,total bile acids(TBA)and serum iron(P<0.05), and less proportion of eosinophils and lower level alkaline phosphatase(P< 0.05). Conclusions The age,the pattern of liver injury,the course of liver damage and aminotransferase levels in patients with drug induced hepati-tis caused by TCM and western medicines were similar;however,western medicines were more likely to cause al-lergic responses and hyperbilirubinemia occurred more frequently in patients with drug induced hepatitis caused by TCM.
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Objective To determine the level of anticyclophilin A auto-antibodies (ACA) in sera of patients with psoriasis. Methods Cyclophilin A (CyPA) was extracted and purified from bovine thymus. The level of anticyclophilin A auto-antibodies (ACA) was detected by enzyme-linked immunosorbent assay (ELISA) and immunoblotting test (IBT) in sera of patients with psoriasis. Results It was shown that the level of ACA was significantly higher in the sera of patients with psoriasis than those in control group (IBT:P<0.01,ELISA:P< 0.01). ACA was found to be positive in 41.3％ of the patients by ELISA. In psoriatic group, the serum level of ACA was higher during the progressive stage than that in the stationary stage, however, no significant difference was observed (IBT:P>0.05,ELISA:P >0.05). Conclusion The results suggest that there is autoimmunological mechanism in the pathogenesis of psoriasis.
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Objective To study the cytokines secreted by fibroblasts in the pathogenesis of psoriasis.Methods The healthy or psoriatic fibroblasts were co-cultured with keratinocytes in skin equivalents for15days.Changes in the expression pattern of268selected genes related to cytokines and their receptors were determined by comparing mRNA levels of fibroblasts under different culture conditions.Results Sixty-eight of268genes were found to be expressed on the fibroblasts.Compared with normal controls,a total of27genes was found to be differentially expressed at RNA level by a factor of twofold or greater,among which24genes were up-regulated,whereas3genes were down-regulated in psoriatic fibroblasts.These differentially expressed genes encoded interleukins,interferons,growth factors,chemokines,protein kinase receptors and chemokine receptors.Conclusions These results suggest that fibroblasts from psoriatic uninvolved skin strongly express cytokines which closely relate to the pathogenesis of psoriasis.It is indicated that dermal fibroblasts may play a potentially role in promoting keratinocyte proliferation and leading to the formation of psoriatic lesion.
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0.05). Conclusion The results suggest that there is autoimmunological mechani sm in the pathogenesis of psoriasis.