RÉSUMÉ
<p><b>OBJECTIVE</b>To determine whether tanycytes be able to support the regeneration of completely transected spinal cord in adult rats.</p><p><b>METHODS</b>Subcultured tanycytes was transplanted into completely T8 transected spinal cord using the untranslated completely transected rats as control. After transplantation the rubrospinal motor evoked potentials were recorded below the injury level at the end of 12th week, assistant by Basso-Beatie-Bresnahan locomotor rating scale and histology method.</p><p><b>RESULTS</b>At the end of 12th week the total peak amplitude of rubrospinal motor evoked potentials (MD = 133.2 microV, P < 0.01) and BBB locomotor rating scale (MD = 5.0000, P < 0.01) were both significantly improved in cell transplanted group compared with that in the untranslated control group, while the latency of the first peak was shortened (MD = 0.061 ms, P = 0.040). HE staining showed more integrity in transected spinal cords in cells transplanted groups.</p><p><b>CONCLUSION</b>Transplanted tanycytes can support the regeneration of transected spinal cords in rats.</p>
Sujet(s)
Animaux , Mâle , Rats , Transplantation cellulaire , Cellules cultivées , Potentiels évoqués moteurs , Névroglie , Biologie cellulaire , Transplantation , Rat Wistar , Récupération fonctionnelle , Traumatismes de la moelle épinière , Chirurgie généraleRÉSUMÉ
<p><b>BACKGROUND</b>Transplanting a vascularized autologous submandibular gland (SMG) is considered an effective method to treat severe keratoconjunctivitis sicca. But the operation may fail due to the anatomic variances in the blood vessels of SMG. The present study aimed to investigate the submandibular glands at the microanatomy level.</p><p><b>METHODS</b>The microanatomy of blood vessels including arteries and veins of submandibular gland was investigated using 30 adult corpses and 60 submandibular glands were anatomized under a surgical microscope. The lengths and diameters of the arterial and venous glandular branches were measured using sliding caliper.</p><p><b>RESULTS</b>The submandibular gland was mainly supplied by the facial artery and submental artery, partly by the lingual artery and external jugular artery. The venous drainage of the submandibualr gland occurred through the anterior facial vein, the venae comitantes of facial artery, the vein close to the Whaston's duct (the hilum vein), and seldom drained to external jugular vein and other veins.</p><p><b>CONCLUSIONS</b>The anatomy of SMG is a complicated structure. Determining the main blood vessels of the submandibular gland is very important to achieve a successful vascularized autologous SMG transplant.</p>