Low-dose reduced glutathione promotes umbilical cord blood mesenchymal stem cell proliferation / 中国组织工程研究

BACKGROUND:How to obtain a sufficient number of cells is one of the key issues in the celltransplantation therapy, and studies have shown that stem cellproliferation can be promoted by reasonable stimulus. OBJECTIVE:To investigate reduced glutathione effects on biological characteristics of human umbilical cord blood mesenchymal stem cells. METHODS:The cells were divided into two groups:the control group consisted of the normal human umbilical cord blood mesenchymal stem cells, and in the experimental group, human umbilical cord blood mesenchymal stem cells were treated with 0.15 g/L reduced glutathione. RESULTS AND CONCLUSION:At days 5, 7, 9, cells treated with 0.15 g/L reduced glutathione showed higher absorbance values than those in the control group (P<0.05). Flow cytometry showed reduced glutathione had no effects on CD29, CD44, CD45, CD105 expression. Real-time PCR results showed reduced glutathione was capable of promoting extracellular signal-regulated kinase mRNA expression (P<0.05). Findings from this study showed that 0.15 g/L reduced glutathione can promote the proliferation of human umbilical cord blood mesenchymal stem cells.

Gene therapy of rat prolactinomas mediated by adenoviral vectors with rat tyrosine hydroxylase gene / 中国医学科学院学报

<p><b>OBJECTIVE</b>To investigate the potential of gene therapy of rat prolactinomas mediated by adenoviral vectors with a gene encoding rat tyrosine hydroxylase.</p><p><b>METHODS</b>Recombinant replication-deficient adenovirus named Ad-GFP-TH with rat TH-cDNA and control adenovirus named Ad-GFP were constructed by homologous recombination in bacterial cells. The rat pituitary prolactinoma cell line MMQ are chosen as the target cells to study the effect of gene therapy on their growth and prolactin secretion mediated by Ad-GFP-TH.</p><p><b>RESULTS</b>Recombinant Ad-GFP-TH and Ad-GFP were successfully reconstructed. Transfection of MMQ cells with Ad-GFP-TH not only restrained their growth but also decreased their PRL secretion.</p><p><b>CONCLUSION</b>Gene therapy may serve for a potential treatment for prolactinomas, especially invasive prolactinomas.</p>

The transcriptional regulation study on human delta globin gene with CAAT box C-->T point mutation in its promoter / 中国医学科学院学报

<p><b>OBJECTIVE</b>To study the transcriptional regulation of human delta globin gene with C-->T point mutation at -64 in its promoter.</p><p><b>METHODS</b>Human delta globin genes including wild CAAT box and mutant CAAT box (-64C-->T) were separately cloned into eukaryotic expression vector pcDNA3.1 (-)/Myc-His A which was cut out the strong promoter CMV, transfected MEL cells, and induced by DMSO to express. The transcriptional regulation of human delta globin gene was analysed using semi-quantitative RT-PCR.</p><p><b>RESULTS</b>The expression level of human delta globin gene with mutant CAAT box was 2.2-fold as high as that with wild CAAT box.</p><p><b>CONCLUSION</b>The defective CAAT box of human delta globin gene promoter region may be one of the major reasons for its low expression level.</p>