Solitaire AB Stent-Assisted Coiling of Wide-Neck Micro Aneurysms

OBJECTIVE: Solitaire AB stent-assisted coiling facilitates the endovascular treatment of wide-necked intracranial aneurysms. We present our experience of coiling the micro-aneurysms of wide-neck with Solitaire AB stent assisting in a single center. METHODS: Thirty-one Solitaire AB stents were used to treat via endovascular approach patients with 31 wide-neck micro aneurysms in a single center in China. Technical and clinical complications were recorded. Modified Rankin Scale was used to evaluate the patients' conditions via clinic and telephone follow-up. RESULTS: The mean width of aneurysm sac was 2.30±0.42 mm, and the mean diameter of aneurysm neck was 2.83±0.48 mm. Complete occlusion was achieved in 28 aneurysms (90.32%); neck remnant was seen in 3 aneurysms (9.68%). Technical and clinical complications related to the procedure were encountered in four patients (12.5%). Two patients died (6.25%). No patient had a permanent deficit. CONCLUSION: Solitaire AB stent was a safe and efficiency tool in assisting coiling of micro aneurysms with wide neck, but may be not suitable for a blaster-like one. Mid- and long-term follow-up will be required to elucidate the impact of the Solitaire AB stent on recanalization rate.

Analysis of genetic diversity of wild Rehmannia glutinosa by using RAPD and ISSR markers / 中国中药杂志

<p><b>OBJECTIVE</b>To analyze the genetic diversity of wild Rehmannia glutinosa and evaluate and compare random amplified polymorphic DNA (RAPD) and inter sample sequence repeat (ISSR) for analysis of R. glutinosa accessions.</p><p><b>METHOD</b>Two molecular markers, RAPD and ISSR were used for analyzing 55 wild R. glutinosa accessions.</p><p><b>RESULT</b>Average 16.00 and 19.08 bands were amplified by RAPD primers and ISSR primers respectively, and the percentage of polymorphic bands were 89.58% and 94.32% respectively; Fifty-five R. glutinosa accessions categorized into 7 clusters were identified by unweighted pair-group method, arithmetic average (UPGMA) method.</p><p><b>CONCLUSION</b>A high level of genetic diversity of wild Rehmannia glutinosa was displayed at DNA level, and genetic diversity coefficient of R. glutinosa from different production areas was 0.63-0.98, and ISSR marker can detect higher genetic diversity of R. glutinosa germplasms than RAPD marker.</p>

AFLP fingerprinting of Pinellia ternata and its application / 中国中药杂志

<p><b>OBJECTIVE</b>To obtain more information on DNA fingerprintings and to confirm the genetic relationships of ten wide populations of Pinellia tornata.</p><p><b>METHOD</b>Collect wide-type or cultivated P. tornata from ten major production areas nation-wide as material. Twelve combinations of EcoR I and Mse I primers were used to asses divergence among ten populations. The data were analyzed using unweighted pair-group method, based on arithmetic averages (UPGMA) bootstrap analysis. Cluster analyses were performed by using NTSYSpc software version 2.1. The alkaloid was extracted from P. ternate with chlorolform and determined in UV absorption spectroscop.</p><p><b>RESULT</b>1,673 bands were applied with 12 combinations. These results of cluster were congruent with differences in total alkloids.</p><p><b>CONCLUSION</b>The results of the study showed a potential application of AFLP fingerprinting for identification of P. ternata.</p>