Antisaccade study of executive functions in children with two subtypes of attention deficit hyperactivity disorder / 中华行为医学与脑科学杂志

Objective To analyze inhibitions and working memory impairments in children with different subtypes of attention deficit hyperactivity disorder (ADHD) with saccade tasks. Methods Participants,aged 10～ 13 years with ADHD-combined ( n= 19) ,ADHD-inattentive ( n= 11 ) and control subjects ( n= 18) ,were selected according to DSM-Ⅳ diagnosis,Conners teacher rating scale and parent symptom questionnaire from 1500pupils from 2 primary schools in Jinhua city by stratified cluster sampling method. Participants performed 3 different saccade tasks: 1 prosaccade and 2 antisaccades executed in a single-task and a dual-task situation. One of the antisaccade tasks was performed under the verbal secondary task condition to investigate verbal working memory.Saccades were registered by Eyelink Ⅱ Tracker. Errors and correct rate were analyzed. One-way ANOVA and repeated measures ANOVA were used to analyze the data. Results (1) Each group showed more response suppression errors on antisaccade task without load than on prosaccade task, ADHD-combined and ADHD-inattentive exhibited more errors than non-ADHD (P＜0.01) ,and errors was lower in ADHD-inattentive than in ADHD-combined ( ( 70.90 ± 15.78 ) % vs ( 84.04 ± 12.84 ) %, P ＜ 0.05 ). (2) The interactions between group and load were not significant in error rate on antisaccade tasks (P ＞ 0.05 ), but in correct rate (P ＜ 0. 05 ). The two subtypes of ADHD were affected more than non-ADHD, their correct rates tended to decrease under verbal working memory load. Conclusion An integrative view of deficits in inhibition and verbal working memory in two subtypes of ADHD is proposed, and ADHD-inattentive has a better response suppression than ADHD-combined.

Study on determination method of the rhG-CSF biological activity / 中国生化药物杂志

Purpose　The determination method of rhG-CSF bio logical activity by NFS-60 cells was studied in accordance with WHO internation al standard for G-CSF.Methods　MTT method was adopted.R esults　The chomosone number of NFS-60 cell was 39, about 1×105 cells/ml to 1×107 cells/ml,the dying color showed gradient when the NFS-60 c ells was dyed by MTT,the method(4,4) was adopted in the determination of rhG-CS F biological activity,the average FL% of potency was 13.560% for single exprimen t,and the CV of inter-assay and intra-assay was lower than 10% and 10.109%,respectively.Conclusion　method(4,4) can be us ed in the determination of rhG-CSF biological activity,and the results can guid e the study and the manufacture of rhG-CSF effectively.

Calibrating the working standard for rhG-CSF / 中国生化药物杂志

Purpose　Both the working standard and corresponding sample(GRAN75)were first calibrated by WHO international standard for G-CSF.Methods　MTT method by NFS-60 cells was used. The results were calculated by (4,4)method.Results　Three batchs working standards were prepared,two batchs were freeze-dry and the prescription was same as WHO international standard for G-CSF,one batch was injection and the prescription was same as corresponding sample(GRAN75).The biological potency and the FL% of average potency were 3.062×106 、4.276×106IU/ampoule、1.635×107IU/ml and 5.529%、4.291%、4.244% for working standard, and 1.880×107IU/ml and 5.175% for corresponding sample,respectively.Conclusion　The working standard which calibrated could be used as working standard in the measurement of rhG-CSF biological activity.