Your browser doesn't support javascript.
loading
Montrer: 20 | 50 | 100
Résultats 1 - 5 de 5
Filtre
Ajouter des filtres








Gamme d'année
1.
Chinese Journal of Information on Traditional Chinese Medicine ; (12): 78-82, 2016.
Article Dans Chinois | WPRIM | ID: wpr-486290

Résumé

Objective To study the difference ofLeonurus japonicus germplasm resources and provide good materials for breeding.Methods Totally 20 wildLeonurus japonicus germplasm resources from different places of China were collected. Experiment was conducted in homogeneous garden in Quzhou City of Zhejiang Province. Phenological phase, cold endurance and habitat adaptability were observed; the plant height, fresh weight and dry weight in early flowering were measured; the contents of stachydrine hydrochloride and leonurine hydrochloride were determined in early flowering and out-of-season cultivation.Results Considering the habitat adaptability, dry plant weight in early flowering, the contents of stachydrine hydrochloride and leonurine hydrochloride separately in early flowering and out-of-season cultivation, it was believed that the germplasms from Linbao County, Sheqi County in Henan Province and Guidong County in Hunan Province were better, in which Linbao germplasm was the best: the dry plant weight was 30.6 g, the content of stachydrine hydrochloride and leonurine hydrochloride were 1.31% and 0.19% respectively in early flowering, and were 3.44% and 0.37% respectively under anti-season cultivation, and it can be well adapted in Zhejiang Province.Conclusion The germplasm ofLeonurus japonicas from Lingbao can be the best materials for breeding.

2.
Chinese Journal of Biotechnology ; (12): 1118-1129, 2012.
Article Dans Chinois | WPRIM | ID: wpr-342411

Résumé

Knockout is an important method for gene function study, while vector is the core of gene knockout. In order to obtain an effective vector for rapid construction of mutant and essentiality identification of the corresponding gene, we constructed a recombinant plasmid named plDM-T based on the temperature-sensitive and replication- defective plasmid plDM1 by inserting an Xcm I adapter into the EcoR I and Pst I sites of pIDM1. Digesting with Xcm I, pIDM-T can be prepared as a linear T-vector for PCR products cloning and be used to knockout the corresponding gene in the genome with insertion duplication mutagenesis. After the verification of temperature sensitivity of the replication of the plasmid, we cloned two Salmonella pullorum genes eno and ybdr into the constructed pIDM-T, and two recombinant plasmids pIDM-T_eno and pIDM-T_ybdr were identified. The recombinant plasmids were then transformed into S. pullorum strain CVCC527 and the IPC (Integration rate per cell) values were calculated. As a result, we identified the eno gene as an essential gene and the ybdr as a non-essential gene in CVCC527. We verified the correctness of recombination site in ybdr recombinant 527 clones (Sal delta ybdr) by PCR and sequencing. The pIDM-T vector can be used for gene knockout in S. pullorum, as well as the identification of essentiality of the corresponding genes, which offers an effective and rapid tool for gene function study in Salmonella.


Sujets)
Clonage moléculaire , Techniques de knock-out de gènes , Vecteurs génétiques , Génétique , Métabolisme , Plasmides , Génétique , Protéines recombinantes , Génétique , Métabolisme , Recombinaison génétique , Salmonella , Classification , Génétique
3.
China Journal of Chinese Materia Medica ; (24): 2484-2488, 2011.
Article Dans Chinois | WPRIM | ID: wpr-293219

Résumé

<p><b>OBJECTIVE</b>To establish integration processing method for pretreating and vinegar producing Corydalis yanhusuo.</p><p><b>METHOD</b>Different processing methods were contrasted with the traditional processing technology, and contents of corydalis B, water extract and ethanol extract in samples of different processing products were determined.</p><p><b>RESULT</b>The content of corydalis B were best in the samples of vacuumizing C. yanhusuo chips scaked in rice vinegar for twice or soaked in rice vinegar after chip drying. The water extract was highest in the samples of chip soaked in rice vinegar after drying, followed with chip vacuumizing twice, and there were no remarkable difference between the other samples and the traditional process. The difference of ethanol extract was not remarkable in all the samples.</p><p><b>CONCLUSION</b>The study provide the feasibility of C. yanhusuo producing and concocting integration processing.</p>


Sujets)
Acide acétique , Chimie , Alcaloïdes de type berbérine , Métabolisme , Corydalis , Chimie , Métabolisme , Lyophilisation , Méthodes , Extraits de plantes , Métabolisme , Plantes médicinales , Chimie , Rhizome , Chimie , Métabolisme , Extraction en phase solide , Méthodes , Eau , Chimie
4.
China Journal of Chinese Materia Medica ; (24): 260-263, 2009.
Article Dans Chinois | WPRIM | ID: wpr-298422

Résumé

<p><b>OBJECTIVE</b>To explore the variety of the genetic polymorphism of eight Prunella germplasm resources by AFLP analysis.</p><p><b>METHOD</b>The amplified fragment length polymorphism (AFLP) tags were applied to screen out 32 selective amplification primer pairs, the amplified bands as original matrix were analyzed with NTSYS-PC software for the similarity between the Prunella germplasm and the construction of genetic phylogenetic tree.</p><p><b>RESULT</b>SDS extraction of genomic Prunella DNA showed a good quality, could meet the requirements of AFLP analysis. From 32 selective amplification primer pairs, 10 pairs with strong polymorphism, better band and higher resolution were used for the construction of the AFLP Prunella fingerprint, all eight Prunella germplasms were separated, they were divided into 3 categories.</p><p><b>CONCLUSION</b>Prunella germplasm resources are rich in genetic diversity, certain morphological characteristics and differences are associate with genotype.</p>


Sujets)
Analyse de polymorphisme de longueur de fragments amplifiés , ADN des plantes , Variation génétique , Polymorphisme génétique , Prunella , Classification , Génétique
5.
Journal of Chongqing Medical University ; (12)1986.
Article Dans Chinois | WPRIM | ID: wpr-580643

Résumé

0.05),but toxicity,especially hematologic toxicity in standard-dose group was higher than that in the low-dose group(P

SÉLECTION CITATIONS
Détails de la recherche