RÉSUMÉ
Objective @#In the theories of pulse disgnosis in traditional Chinese medicine (TCM), it is emphasized that pulse manifestations at the radial artery within the wrist (called Cunkou) signify the physiological and pathological conditions of different internal organs in the human body. However, different opinions exist among researchers about the objectiveness of the pulse diagnosis technique. Some researchers mentioned that no significant differences were observed in pulse manifestations at various Cunkou areas, hence there might be some difficulty in evaluating the status of different organs through checking pulse manifestations at Cunkou. This research aims to analyze the pulse response at Cunkou from the aspect of the characteristics of tactile sensing, thus to give a preliminary explanation to the above question.@*Methods@#This research utilized the Weber-Fechner law to model the tactile sensing as a dynamic low-pass signal filter with varying bandwidths under different compression levels during pulse diagnosis. The model was applied to analyzing the clinical data collected previously by our group. The arterial pressures measured invasively with equipment from 14 patients with aorta coarctation were processed to simulate different pulse manifestations at Cun, Guan, and Chi positions of Cunkou when different compression levels were applied.@*Result@#Due to the characteristics of tactile sensing, significant variations were observed in pulse manifestations at different pulse-depths under the application of changing compression levels; while no such changes in pulse manifestations were observed from the employment of transducer only, without tactile sensing involved. The results explained why different understandings on pulse manifestations were formed between direct pulse-taking technique in TCM and modern sphygmography using transducers. The features of pulse manifestations at Cunkou, using direct pulse-taking technique but at different depths, superficial, middle, and deep positions, respectively, predicted by the developed tactile sensing model were in line with those described in TCM pulse theories.@*Conclusion@#Based on the developed tactile sensing model, this study preliminarily explains the phenomenon that pulse manifestation at Cunkou changes in response to the compression force applied during TCM pulse-taking. Integrating the tactile sensing model with the study of TCM pulse diagnosis opens a new chapter for visualizing and quantitatively interpreting pulse manifestations. This not only expands the scope of pulse diagnosis study effectively, but also provide a scientific basis for further technical upgrading and optimization of existing pulse diagnosis equipment.
RÉSUMÉ
To examine the changes in matrix metalloproteinase-2(MMP-2)and-9(MMP-9)in the development and progression of endometriosis,real time quantitative polymerase chain reaction,enzyme-linked immunoabsorbent assay and gelatin zymography were employed to determine the mRNA and protein levels and activities of MMP-2 and MMP-9 from the first day to the 21st day after the induction in mice with induced endometriosis(experimental group)and sham-operated animals (controls).The results showed that the mRNA and protein levels and activities of the MMP-2 and MMP-9 were significantly increased on the first day after the induction and the level of MMP-2 stayed at a level higher than that in the control group.MMP-9 had two or three peaks during the 21 days,taking place at day 1,4 and 15.It is concluded that the changes in the MMP-2 and MMP-9 might be involved in pathogenesis of endometriosis.