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1.
Acta Pharmaceutica Sinica ; (12): 704-712, 2024.
Article Dans Chinois | WPRIM | ID: wpr-1016613

Résumé

The objective of this study was to analyze the effects on cell viability, apoptosis, and cell cycle of non-small cell lung cancer (NSCLC) A549 cells after intervention with Agrimonia pilosa (AP) and investigate Agrimonia pilosa anti-tumor activity in vitro. Meanwhile, liquid chromatography mass spectrometry (LC-MS) metabolomics technology was used to analyze the changes of cellular metabolites and metabolic pathways. The results of this study will provide a theoretical and experimental basis for investigating the mechanism of the effect of Agrimonia pilosa on non-small cell lung cancer A549 cells. The results showed that the cell nucleus of A549 cells crumpled and apoptosis occurred with the increase of drug concentration. The survival rate of the cells decreased, and the inhibition rate reached 21.5% and 91.74% under the low and high dose conditions, respectively. Lactate dehydrogenase (LDH) content increased (P < 0.05). Metabolomics results showed significant differences in metabolism between groups, thirty-three distinct metabolites including LysoPC(24:0/0:0), LysoPC(17:0/0:0) and PC(O-40:5) were deduced. The pathway enrichment showed that the Agrimonia pilosa plays an anti-tumor role mainly by regulating the metabolism of glycerophosphate and purine in A549 cells, in which the effect on glycerophosphate metabolism pathway was most significant. The results of combined pharmacodynamics suggested that Agrimonia pilosa might induce apoptosis and inhibit the growth of A549 cells by regulating LysoPC(24:0/0:0), LysoPC(17:0/0:0) and PC(O-40:5) metabolites in A549 cells.

2.
Chinese Journal of Natural Medicines (English Ed.) ; (6): 499-504, 2018.
Article Dans Anglais | WPRIM | ID: wpr-773591

Résumé

Two previously undescribed steroidal compounds, 16, 23-epoxy-22, 26-epimino-cholest-22(N), 23, 25(26)-trien-3β-ol-3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside (1) and 26-O-β-D-glucopyranosyl-(25R)-5α-furost-20(22)-en-3β, 26-diol (2), together with 7 known ones including 26-O-β-D-glucopyranosyl-(25R)-5, 20(22)-dien-furost-3β, 26-diol (3), (25R)-5-en-spirost-3β-ol-O-β-D-glucopyranosyl-(1→4)-[α-L-rhmanopyranosyl-(1→2)]-β-D-galactopyranoside (4), funkioside D (5), aspidistrin (6), tigogenin-3-O-β-D-lucotrioside (7), desglucolanatigonin II (8), and degalactotigonin (9), were isolated from Solanum lyratum Thunb. Their cytotoxic activities were tested in two cancer cell lines by MTT method. One of the steroidal glycosides (6) showed significant cytotoxic activity against gastric cancer SGC7901 and liver cancer BEL-7402 cells.


Sujets)
Humains , Alcaloïdes , Chimie , Toxicité , Antinéoplasiques , Chimie , Toxicité , Lignée cellulaire tumorale , Survie cellulaire , Hétérosides , Chimie , Pharmacologie , Toxicité , Concentration inhibitrice 50 , Structure moléculaire , Phytostérols , Chimie , Toxicité , Extraits de plantes , Chimie , Toxicité , Plantes médicinales , Chimie , Solanum , Chimie , Stérols , Chimie , Pharmacologie , Toxicité
3.
Chinese Journal of Natural Medicines (English Ed.) ; (6): 499-504, 2018.
Article Dans Anglais | WPRIM | ID: wpr-812380

Résumé

Two previously undescribed steroidal compounds, 16, 23-epoxy-22, 26-epimino-cholest-22(N), 23, 25(26)-trien-3β-ol-3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside (1) and 26-O-β-D-glucopyranosyl-(25R)-5α-furost-20(22)-en-3β, 26-diol (2), together with 7 known ones including 26-O-β-D-glucopyranosyl-(25R)-5, 20(22)-dien-furost-3β, 26-diol (3), (25R)-5-en-spirost-3β-ol-O-β-D-glucopyranosyl-(1→4)-[α-L-rhmanopyranosyl-(1→2)]-β-D-galactopyranoside (4), funkioside D (5), aspidistrin (6), tigogenin-3-O-β-D-lucotrioside (7), desglucolanatigonin II (8), and degalactotigonin (9), were isolated from Solanum lyratum Thunb. Their cytotoxic activities were tested in two cancer cell lines by MTT method. One of the steroidal glycosides (6) showed significant cytotoxic activity against gastric cancer SGC7901 and liver cancer BEL-7402 cells.


Sujets)
Humains , Alcaloïdes , Chimie , Toxicité , Antinéoplasiques , Chimie , Toxicité , Lignée cellulaire tumorale , Survie cellulaire , Hétérosides , Chimie , Pharmacologie , Toxicité , Concentration inhibitrice 50 , Structure moléculaire , Phytostérols , Chimie , Toxicité , Extraits de plantes , Chimie , Toxicité , Plantes médicinales , Chimie , Solanum , Chimie , Stérols , Chimie , Pharmacologie , Toxicité
4.
Chinese Journal of Natural Medicines (English Ed.) ; (6): 300-304, 2014.
Article Dans Anglais | WPRIM | ID: wpr-812260

Résumé

AIM@#To study the chemical constituents of stems of Gymnema sylvestre (Retz.) Schult.@*METHODS@#Chromatographic techniques using silica gel, C18 reversed phase silica gel, and prep-HPLC were used. The structures were elucidated on the basis of MS and spectroscopic analysis (1D and 2D NMR), as well as chemical methods.@*RESULTS@#Seven compounds were isolated and their structures were elucidated as conduritol A (1), stigmasterol (2), lupeol (3), stigmasterol-3-O-β-D-glucoside (4), the sodium salt of 22α-hydroxy-longispinogenin-3-O-β-D-glucopyranosyl-(1→3)-β-D-glu-curono-pyranosyl-28-O-α-L-rhamnopyranoside (5), oleanolic acid-3-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside (6), and the sodium salt of 22α-hydroxy-longispinogenin 3-O-β-D-glucuronopyranosyl-28-O-α-L-rhamnopyranoside (7). The inhibition activities of compounds 1, 5-7 on non-enzymatic glycation of protein in vitro were evaluated.@*CONCLUSION@#Compound 7 is a new triterpenoid saponin. It was shown that compounds 1, 5-7 have weak inhibition activities for non-enzymatic glycation of protein in vitro.


Sujets)
Médicaments issus de plantes chinoises , Chimie , Gymnema sylvestre , Chimie , Structure moléculaire , Tiges de plante , Chimie
5.
Chinese Journal of Stomatology ; (12): 681-685, 2009.
Article Dans Chinois | WPRIM | ID: wpr-274515

Résumé

<p><b>OBJECTIVE</b>To investigate the effect of cytoskeleton integrity on the expression of c-fos gene in osteoblasts induced by fluid shear stress.</p><p><b>METHODS</b>BALB/c mouse primary osteoblasts were divided into four groups (according to fluid shear stress loaded or not and cytochalasin D used or not). The Tagman probe real-time PCR and immunofluorescence were performed to detect the expression levels of c-fos mRNA, c-fos protein and cytoskeleton, respectively. The data were analysed using two-way ANOVA.</p><p><b>RESULTS</b>In control group and cytochalasin D group, fluid shear stress could significantly increase the expression levels of c-fos mRNA (0.1637 +/- 0.0303 and 0.0104 +/- 0.0070, respectively) and protein (177.14 +/- 9.37 and 150.95 +/- 6.17, respectively) in osteoblasts, compared with the unloaded osteoblasts of the control group and the cytochalasin D group (0.0057 +/- 0.0021 and 0.0032 +/- 0.0014, respectively for c-fos mRNA, and 117.96 +/- 4.11 and 119.77 +/- 5.19, respectively for protein, P < 0.05). Induced by the fluid shear stress, the expression levels of c-fos mRNA and protein in cytochalasin D group were lower than control group, and the difference had statistical significance (P < 0.05).</p><p><b>CONCLUSIONS</b>The cytoskeleton integrity in osteoblasts was essential to the expression of c-fos gene induced by fluid shear stress.</p>


Sujets)
Animaux , Souris , Analyse de variance , Cellules cultivées , Cytochalasine D , Pharmacologie , Cytosquelette , Physiologie , Souris de lignée BALB C , Ostéoblastes , Métabolisme , Protéines proto-oncogènes c-fos , Métabolisme , ARN messager , Métabolisme , Rhéologie , Contrainte mécanique
6.
China Journal of Chinese Materia Medica ; (24): 988-992, 2004.
Article Dans Chinois | WPRIM | ID: wpr-293654

Résumé

<p><b>OBJECTIVE</b>To compare the effect of the extracts from Decoction for resuscitation (DRE) and its component herbs on prostacyclin (PGI2), thromboxane A2 (TXA2) and nitric oxide (NO) release from rat vascular endothelial cells under hypoxia.</p><p><b>METHOD</b>After treatment with the extracts from DRE and its component herbs, the contents of 6-keto-prostaglandin F1alpha(6-keto-PGF1alpha), thromboxane B2 (TXB2) as well as nitrite (NO), which were degradation products of PGI2, TXA2 and NO respectively, in culture medium of rat vascular endothelial cells under hypoxia were measured with radioimmunoassay and Griess Reaction.</p><p><b>RESULT</b>Compared with the control group, the results indicated that DRE, prepared licorice root extract (LE), dried ginger extract (GE), aconite root extract (AE), extracts of aconite root and prepared licorice root (ALE), extracts of aconite root and dried ginger (AGE) increased significantly the content of 6-keto-PGF1alpha and the ratio of 6-keto-PGF1alpha/TXB2, but had no effect on the content of TXB2 in culture medium of rat vascular endothelial cells under hypoxia. The content of 6-keto-PGF1alpha in the DRE group was higher than that in the groups of LE, GE, AE, ALE, AGE. The ratio of 6-keto-PGF1alpha/TXB2 in the DRE group was higher than that of the groups of GE, AE, ALE. Compared with the control group, DRE, LE, GE, AE, ALE, AGE increased significantly the content of NO2- in culture medium of rat vascular endothelial cells under hypoxia. Moreover, the content of NO2- in the DRE group was higher than that of the groups of GE, AE, ALE.</p><p><b>CONCLUSION</b>The results suggested that DRE increased significantly the content of PGI2 and the ratio of PGI2/TXA2 as well as the content of NO. The effect of DRE on the parameters in culture medium of rat vascular endothelial cells under hypoxia was better than that of the extracts from its component herbs.</p>


Sujets)
Animaux , Rats , 6-Cétoprostaglandine Fl alpha , Métabolisme , Aconitum , Chimie , Aorte abdominale , Biologie cellulaire , Hypoxie cellulaire , Médicaments issus de plantes chinoises , Pharmacologie , Cellules endothéliales , Métabolisme , Zingiber officinale , Chimie , Glycyrrhiza uralensis , Chimie , Monoxyde d'azote , Métabolisme , Plantes médicinales , Chimie , Rat Wistar , Thromboxane B2 , Métabolisme
7.
China Journal of Chinese Materia Medica ; (24): 742-782, 2002.
Article Dans Chinois | WPRIM | ID: wpr-271827

Résumé

<p><b>OBJECTIVE</b>To study the chemical constituents of Ginseng Sini Tang.</p><p><b>METHOD</b>The constituents were identified by physico-chemical properties and spectral analysis.</p><p><b>RESULT</b>The 12 compounds were identified as ginsenoside-Rb1,-Rb2,-Rb3,-Rc,-Rd,-Re,-Rg1,Rg2,Rg3,Rf,Ra1,Ra2. The 10 compounds were identified as benzoylmesaconitine(BM), benzoylaconitine(BA), benzoylhypaconitine(BH), neoline (NL), fuziline (FL), 14-ethyl-talatisamine14-acetyl-talatisamine (AT), 14-benzoylhypaconine-8-linoleate (HAL),14-benzoyldeoxyaconine-8-oleate(HAO), 14-benzoylhypaconine-8-palmitate(HAP), talatisamine(TS).</p><p><b>CONCLUSION</b>All these compounds were obtained from Ginseng Sini Tang for first times.</p>


Sujets)
Animaux , Alcaloïdes , Pharmacologie , Dépression chimique , Association médicamenteuse , Médicaments issus de plantes chinoises , Pharmacologie , Ginsénosides , Pharmacologie , Contraction myocardique , Panax , Chimie , Plantes médicinales , Chimie
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