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Journal of Experimental Hematology ; (6): 672-680, 2016.
Article Dans Chinois | WPRIM | ID: wpr-360027

Résumé

<p><b>OBJECTIVE</b>To investigate the apoptotic effects of Hsp90 selective inhibitor 17-AAG on human leukemia HL-60 and NB4 cells and analyse its possible mechanism.</p><p><b>METHODS</b>CCK-8 assay was used to quantify the growth inhibition of cells after exposure to 17-AAG for 24 hours. Flow cytometrve with annexin V/propidium iodide staining was used to detect apoptosis of leukemia cells. Then Western blot was used to detect the activation of apoptosis related protein caspase-3 and PARP level. Gene expression profile of NB4 cells treated with 17-AAG was analyzed with real-time PCR arrays.</p><p><b>RESULTS</b>The inhibition of leukemia cell proliferation displayed a dose-dependent manner. Annexin V assay, cell cycle analysis and activation of PARP demonstrate that 17-AAG induced apoptosis leukemia cells. Real-time PCR array analysis showed that expression of 56 genes significantly up-regulated and expression of 23 genes were significantly down-regulated after 17-AAG treatment.</p><p><b>CONCLUSION</b>The 17-AAG can inhibit the proliferation and induce the apoptosis of leukemia cells. After leukemia cells are treated with 17-AAG, the significant changes of apoptosis-related genes occured, and the cell apoptosis occurs via activating apoptosis related signaling pathway.</p>


Sujets)
Humains , Apoptose , Benzoquinones , Pharmacologie , Caspase-3 , Métabolisme , Cycle cellulaire , Lignée cellulaire tumorale , Prolifération cellulaire , Régulation négative , Cellules HL-60 , Protéines du choc thermique HSP90 , Lactames macrocycliques , Pharmacologie , Leucémies , Métabolisme , Poly(ADP-ribose) polymerases , Métabolisme , Réaction de polymérisation en chaine en temps réel , Transduction du signal , Transcriptome
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