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Chinese Journal of Oncology ; (12): 521-524, 2004.
Article Dans Chinois | WPRIM | ID: wpr-254311

Résumé

<p><b>OBJECTIVE</b>To study the effect of overexpression of Smad7 gene on cell proliferation in human bronchial epithelial cell lines.</p><p><b>METHODS</b>Human bronchial epithelial cell lines, BEP2D and BERP35T2 cells, were cotransfected with the mammalian expression vectors PCISmad7.neo and pMyc-SEAP, the latter was ac-myc cis-acting enhancer element fused with alkaline phosphatase (SEAP) reporter gene. Expression of c-myc, p15 and p21 mRNA was detected by RT-PCR before and after stable transfection of Smad7 into BEP2D and BERP35T2 cells in order to study the regulation of TGF-beta-mediated growth inhibition.</p><p><b>RESULTS</b>After BEP2D and BERP35T2 cells transfected with Smad7, the transcriptional activity of c-myc was significantly increased. Smad7 overexpressing cells showed upregulation of c-myc expression and downregulation of p15 and p21 expression, which contributed to the loss of TGF-beta responses in these cells.</p><p><b>CONCLUSION</b>Overexpression of Smad7 may facilitate cell proliferation by antagonizing TGF-beta-mediated antiproliferative gene responses.</p>


Sujets)
Humains , Bronches , Biologie cellulaire , Prolifération cellulaire , Transformation cellulaire néoplasique , Cellules cultivées , Inhibiteur p15 de kinase cycline-dépendante , Génétique , Inhibiteur p21 de kinase cycline-dépendante , Génétique , Cellules épithéliales , Biologie cellulaire , Protéines proto-oncogènes c-myc , Génétique , Transduction du signal , Protéine Smad7 , Génétique , Transfection , Facteur de croissance transformant bêta , Génétique
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