Résumé
To study the new antifungal active triterpene glycosides of sea cucumber Holothuria scabra. Triterpene glycosides from Holothuria scabra were separated and purified by silica gel chromatography, reversed-phase silica gel chromatography and RP-HPLC. Their structures were elucidated on the basis of spectral data and chemical evidence. Three triterpene glycosides were identified as scabraside A (1), echinoidea A (2) and holothurin A1 (3). Scabraside A (1) is a new triterpene glycoside, and compounds 2 and 3 were isolated from Holothuria scabra for the first time. They showed antifungal activities (1 < or = MIC80 < or = 16 microg mL(-1)).
Sujets)
Animaux , Antifongiques , Pharmacologie , Hétérosides , Chimie , Pharmacologie , Holothuria , Chimie , Holothurine , Chimie , Pharmacologie , Structure moléculaire , Triterpènes , Chimie , PharmacologieRésumé
<p><b>OBJECTIVE</b>To study the bioactive constituents from tubers of Bolbostemma paniculatum.</p><p><b>METHOD</b>Compounds were isolated by extraction and partition as well as several-chromatographic techniques guided with Pyricularia oryzae bioassay method. Their structures were determined on the basis of spectral analysis and chemical evidence.</p><p><b>RESULT</b>Bisdesmoside (I) was isolated as active compound causing morphological abnormality of Pyncularia oryzae mycelia and elucidated as 3-0-alpha-L-arabinopyranosyl (1-->2)-beta-D-glucopyranosyl-bayogenin-28-O-beta-D-xylopyranosyl (1-->3)-alpha-L-rhamnopyranosyl (1-->2)-alpha-L-arabinopyranoside.</p><p><b>CONCLUSION</b>I is a new natural product and exhibited significant cytotoxicity against cancer cell lines K-562 and BEL-7402, but no hemolytic activity to rabbit erythrocytes.</p>
Sujets)
Animaux , Humains , Lapins , Dosage biologique , Carcinome hépatocellulaire , Anatomopathologie , Lignée cellulaire tumorale , Prolifération cellulaire , Cucurbitaceae , Chimie , Érythrocytes , Hémolyse , Cellules K562 , Tumeurs du foie , Anatomopathologie , Conformation moléculaire , Structure moléculaire , Tubercules , Chimie , Plantes médicinales , Chimie , Saponines , Chimie , PharmacologieRésumé
<p><b>AIM</b>To separate and identify the chemical constituents of the aril of Torreya grandis cv. Merrilli.</p><p><b>METHODS</b>Three lignins were isolated by chromatography and their chemical structures were elucidated by IR, EI-MS, 1HNMR, 13CNMR, DEPT and 2D-NMR spectral methods.</p><p><b>RESULTS</b>Three lignins were identified as pinonesinol, dihydrodehydrodiconiferylalcohol and (7,8-cis-8,8'-trans)-2',4'dihydroxyl-3, 5-dimethoxy-lariciresinol.</p><p><b>CONCLUSION</b>These compounds were isolated from this plant for the first time, and compound III is a new compound.</p>
Sujets)
Fruit , Chimie , Furanes , Chimie , Lignine , Chimie , Conformation moléculaire , Structure moléculaire , Plantes médicinales , Chimie , Taxaceae , ChimieRésumé
<p><b>AIM</b>To study the bioactive constituents from Anthopleura pacifica.</p><p><b>METHODS</b>Compounds were separated by Pyricularia oryzae bioassay-guided fractionation method with a combination of multi-chromatography. Their chemical structures were determined on the basis of spectral analysis and chemical evidence.</p><p><b>RESULTS</b>A portion showing activity against P. oryzae was obtained and from the portion four compounds were identified as N-hydroxyethyl-N-tetradecanoyl-(2S,3R)-octadecasphinga-4(E), 8(E)-dienine (a), N-hydroxyethyl-N-(9Z-hexadecenoyl)-(2S,3R)-octadecasphinga-4 (E), 8 (E)-dienine (b), N-hydroxyethyl-N-hexadecanoyl-(2S,3R)-octadecasphinga-4(E), 8 (E)-dienine (c) and N-hydroxyethyl-N-(13Z-docosenoyl-(2S,3R)-octadecasphinga-4(E), 8(E)-dienine(d).</p><p><b>CONCLUSION</b>All the four compounds are new ceramides.</p>
Sujets)
Animaux , Dosage biologique , Céramides , Chimie , Venins de cnidaires , Chimie , Deuteromycota , Conformation moléculaire , Structure moléculaire , Anémones de mer , ChimieRésumé
<p><b>AIM</b>To study the chemical constituents of the stem of Cassia siamea.</p><p><b>METHODS</b>The compounds were isolated by chromatography on silica gel, and identified on the basis of spectral analysis.</p><p><b>RESULTS</b>Five compounds were isolated and identified as: beta-sitosterol (I), sucrose (II), n-octacosanol (III), 2-methyl-5-(2'-hydroxypropyl)-7-hydroxy-chromone-2'-O-beta-D-glucopyranoside (IV) and piceatannol (V).</p><p><b>CONCLUSION</b>Compound IV is a new compound. Compounds II, III and V were obtained from this plant for the first time.</p>
Sujets)
Cassia , Chimie , 4H-1-Benzopyran-4-ones , Chimie , Alcools gras , Chimie , Oses , Chimie , Tiges de plante , Chimie , Plantes médicinales , Chimie , Stilbènes , ChimieRésumé
<p><b>OBJECTIVE</b>To study the chemical constituents of Ishige okamurai.</p><p><b>METHOD</b>Compounds were isolated by Pyricularia oryzae bioassay-guided fractionation method in combination with extraction and partitionation as well as multi-chromatography. Their structures were determined by spectral analysis and chemical evidence.</p><p><b>RESULT</b>Seven compounds were obtained and identified as (2S)-1-O-palmitoyl-2-O-(11Z-octadecenoyl)-3-O-beta-D-galacto-pyranosyl glycerol (I), (2S)-1-O-palmitoyl-2-O-myristoyl-3-O-(6-sulfo-alpha-D- quinovopyranosyl) glycerol (II), (2S)-1-O-palmitoyl-2-O-palmitoyl-3-O-(6'-sulfo-alpha-D- quinovopyranosyl) glycerol (III) and (2S)-1-O-palmitoyl-2-O-(11Z-octadecenoyl)-3-O-(6'-sulfo-alpha-D- quinovopyranosyl) glycerol (IV), stearic acid (V), methyl myristate(VI) and palmitic acid (VII).</p><p><b>CONCLUSION</b>Compounds I-VI were isolated from the alga for the first time while I, II and IV are new natural products. I-IV showed activity causing morphological abnormality of P. oryzae mycelia.</p>
Sujets)
Femelle , Humains , Antinéoplasiques , Pharmacologie , Glycolipides , Chimie , Pharmacologie , Cellules HL-60 , Deuteromycota , Structure moléculaire , Phaeophyceae , Chimie , Acides stéariques , Chimie , Cellules cancéreuses en cultureRésumé
<p><b>OBJECTIVE</b>To apply conidia of Pyricularia Oryzae to the screening of antimitotic constituents from marine animal sea hare.</p><p><b>METHOD</b>To extract and fractionate active portions from sea hare through detecting deformation of mycelia germinated from conidia of P. Oryzae P-2b, in comparison with the cytotoxic test results in vitro.</p><p><b>RESULT</b>Two active portions, of which IC50 against P388 and HL-60 was 23.4, 18.6 and 19.4, 12.5 micrograms.ml-1, respectively, were screened from this animal.</p><p><b>CONCLUSION</b>This bioassay method was efficiently applied to the primary screening of antimitotic portions from marine animals for the first time. Being convenient, speedy and cheap, the screening model is suitable for the bioassay of active constituents from marine life.</p>
Sujets)
Animaux , Humains , Souris , Antinéoplasiques , Pharmacologie , Aplysia , Chimie , Cellules HL-60 , Leucémie P388 , Anatomopathologie , Matière médicale , Pharmacologie , Mitose , Deuteromycota , Physiologie , Cellules cancéreuses en cultureRésumé
Objective: To investigate the influence of esculentoside A（EsA） on production of IL-1 and TNF by rabbit synovial cells induced by LPS. Methods: levels of IL-1 and TNF in the supernatant of rabbit synovial cell were determined by examining proliferation of thymic cells and by bioassay L929 cells as target cells, respectively. Results: EsA in 5-40 μg/ml could significantly inhibit the production of IL-1 and TNF from rabbit synovial cells induced by LPS. Conclusion: EsA can inhibit the production of IL-1 and TNF from synovial cells. It suggests that EsA may play a role in improving the rheumatoid arthritis.
Résumé
Objective: To investigate the influence of esculentoside A（EsA） on production of IL-1 and TNF by rabbit synovial cells induced by LPS. Methods: levels of IL-1 and TNF in the supernatant of rabbit synovial cell were determined by examining proliferation of thymic cells and by bioassay L929 cells as target cells, respectively. Results: EsA in 5-40 μg/ml could significantly inhibit the production of IL-1 and TNF from rabbit synovial cells induced by LPS. Conclusion: EsA can inhibit the production of IL-1 and TNF from synovial cells. It suggests that EsA may play a role in improving the rheumatoid arthritis.