Isolation of wound healing compounds from Heliotropium indicum.

Heliotropium indicum (Boraginaceae) is used in the traditional Ivory Coast pharmacopeia to treat asthma. In the present study, wound healing effect of n- butanol fractions was evaluated in H292- cells. Fractions which possessed better wound healing activity were fractionated on Sephadex LH20 column chromatography. Two compounds have been isolated which were responsible for this wound healing effect. Their structures were established as Pestalamide B (1) and Glycinamide,N-(1-oxooctadecyl)glycyl-L-alanylglycyl-L-histidyl (2) on the basis of spectral analysis. Both compound 1 and 2 presented wound healing effect compared with the control (P<0.05).