Construction of vectors encoding mouse Dishevelled 2 and its expression in RAW264.7 cells / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To construct the recombinant vectors that express mouse Dishevelled 2 (Dvl2), and to evaluate its expression level in transfected RAW264.7 cells.</p><p><b>METHODS</b>A pair of specific primers were designed according to the mouse Dvl2 cDNA sequence published in GenBank. Total RNA of RAW264.7 cells was extracted, and open reading frame of Dvl2 was obtained by RT-PCR, which was then cloned into pEZ-M29 plasmid. Electrophoresis after macrorestriction and DNA sequence analysis were used to identify the reconstructed plasmids. After transient transfection via liposome, the transfection of RAW264.7 cells was confirmed under a fluorescence microscope, and the expression level of Dvl2 was evaluated by real-time RT-PCR.</p><p><b>RESULTS</b>The recombinant plasmid containing mouse Dvl2, namely pEZ-M29/ Dvl2, was successfully constructed, and confirmed by DNA sequence analysis. After 48 h of tranfection, the expression of enhanced green fluorescene protein was observed under a fluorescence microscope, and real-time RT-PCR analysis revealed that the Dvl2 mRNA level was prominantly elevated in the transient transfected RAW264.7 cells.</p><p><b>CONCLUSION</b>The recombinant plasmids pEZ-M29/Dvl2 are successfully constructed and can elevate the Dvl2 mRNA level in the transient transfected RAW264.7 cells, which can be used in further studies aiming at revealing the functional significance of Dvl2 in the osteoclastogenesis.</p>

Effect of aurum coating on corrosion resistance of Ni-Cr alloy / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To evaluate the effect of aurum coating on corrosion resistance of Ni-Cr alloy in artificial saliva environment.</p><p><b>METHODS</b>The corrosion potential (E(corr)), self-corrosion current density (I(corr)), and polarization resistance (R(p)) of three alloys were measured using electrochemical methods to compare the difference of corrosion resistance between aurum-coated Ni-Cr alloy and Ni-Cr alloy or Au alloy. Meanwhile, microstructural and phase diffraction was examined with field scanning electromicroscopy (FSEM) and surface chemical analysis was performed by energy diffraction X-ray (EDX).</p><p><b>RESULTS</b>The I(corr) of aurum-coated Ni-Cr alloy was (0.70 +/- 0.20) x 10(-6) A/cm2, which was significantly higher than that of Au alloy (P < 0.05) and lower than that of Ni-Cr alloy (P < 0.05). R(p) of aurum-coated Ni-Cr alloy was (34.77 +/- 12.61) KOmega.cm2, which was higher than that of Ni-Cr alloy (P < 0.05) and lower than that of Au alloy (P < 0.05). The results of FSEM showed that the corrosion resistance of Ni-Cr alloy coated with aurum was better than that of Ni-Cr alloy. The results of EDX indicated that released Ni and Cr of Ni-Cr alloy coated with aurum after test were less than those of Ni-Cr alloy (P < 0.05).</p><p><b>CONCLUSIONS</b>The corrosion resistance of aurum-coated Ni-Cr alloy is higher than that of Ni-Cr alloy.</p>