Résumé
<p><b>OBJECTIVE</b>To study the effect of exogenous cytokine-stimulated decidual cells on IgG secretion of B lymphocytes and investigate the features of local immunological microenvironment of the decidua.</p><p><b>METHODS</b>Exogenous cytokines interferon-gamma (IFN-gamma), interleukin-2 (IL-2), IL-6 and epidermal growth factor (EGF) were added separately in cultured decidual cells, and the supernatant of the culture medium was prepared for stimulating human peripheral blood lymphocytes. IgG secretion of the B cells was measured by radio-immunological method.</p><p><b>RESULT</b>The decidual cells of normal early pregnancy stimulated B lymphocyte IgG secretion, and the supernatant of exogenous cytokine-stimulated decidual cells had the same effect, which, however, was depended not on the concentration and category of the cytokines, but only on the time of treatment.</p><p><b>CONCLUSION</b>The exogenous cytokines can increase the humoral immunity in the decidual immunological microenvironment, but such effect might result from a self-regulatory mechanism of the local immunological microenvironment of the decidua, which can be fundamental for maintenance of normal pregnancy.</p>
Sujets)
Adulte , Femelle , Humains , Grossesse , Lymphocytes B , Biologie cellulaire , Métabolisme , Cellules cultivées , Milieux de culture conditionnés , Pharmacologie , Cytokines , Pharmacologie , Caduques , Biologie cellulaire , Métabolisme , Immunoglobuline G , Interféron gamma , Pharmacologie , Interleukine-2 , Pharmacologie , Premier trimestre de grossesseRésumé
<p><b>OBJECTIVE</b>To study the mechanisim of Yi Qi Tong Lin Chingji in treating benign prostatic hyperplasia.</p><p><b>METHOD</b>The expressions of VEGF, bFGF and TGF beta 1 in prostatic hyperplasia model rats were examined by immunohistochemistry. 8 rats were in contrastive group, 24 influenced by Yi Qi Tong Ling Chingji and Prostacar.</p><p><b>RESULT</b>The expressions of VEGF and bFGF were significantly difference, but the expression of TGF beta 1 was not significant. (P < 0.01). The expression of VEGF and bFGF were significant in contrastive group to the high-dose of Yi Qi Tong Ling Chingji and Prostacar group (P < 0.05) but were not significant to the low-dose of Yi Qi Tong Ling Chingji group. There was no significant difference between high-dose of Yi Qi Tong Ling Chingji and Prostacar group and three growth factors.</p><p><b>CONCLUSION</b>Yi Qi Tong Ling Chingji inhibit the expression of VEGF and bFGF in BPH so as to decrease the volume of prostate.</p>