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Objective@#To investigate the prevalence of witnessing domestic violence,and to explore the relationship between witness domestic violence in childhood and college students’ injury and violence behaviors.@*Methods@#In October 2018, self-made questionnaire on “Health and Risky Behaviors among University Students in Anhui Province” was conducted among 4 034 college students from 4 universities in Hefei. Multivariate Logistic regression models (control of confounding factors) were used to explore the impact of childhood witnessing domestic violence on college students’ injury and violence.@*Results@#Among 4 034 college students,the prevalence of witnessing domestic emotional violence,mild physical violence and severe physical violence in childhood was 27.6%,22.4%,10.7%,respectively. Univariate analysis showed that students with experiences of witness domestic emotional violence,mild physical violence and severe physical violence had higher rates of self-injury,physical,emotional,and sexual violence compared to those without domestic violence witness(P<0.05). Multivariate Logistic regression analysis showed that witnessing domestic severe physical violence,emotional violence in childhood associated positively with self-harm (OR=1.53,95%CI=1.05-2.23; OR=2.15,95%CI=1.51-3.04) and emotional violence (OR=1.65,95%CI=1.16-2.35; OR=2.57,95%CI=1.87-3.53). Witnessing domestic severe physical violence showed positive association with physical violence (OR=4.99,95%CI=2.58-9.62) and sexual violence (OR=8.68,95%CI=3.30-22.81) among college students (P<0.05).@*Conclusion@#The experiences of witness domestic violence can increases the risk of college student’s injury and violence behaviors,especially witness domestic severe physical violence.
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The clinical data of 31 patients with congenital heart disease(CHD)receiving minimally invasive surgical closure through chest or femoral venous catheter from June 2016 to September 2017 were analyzed retrospectively.All patients were diagnosed with esophageal echocardiography before operation.During the operation,the insert of guide wire and the sheath tube,and the placement of the sealing parasol were monitored and guided by transesophageal echocardiography (TEE).Satisfactory results were obtained in 30 patients.In 1 patients,the sealing parasol was detached,and open chest surgery was performed to remove the detached parasol and to repair the defect.TEE-guided minimally invasive surgical closure through chest or femoral vein catheter is safe and effective in the treatment of congenital heart disease and has certain clinical applicative value.
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Objective: To investigate the clinical value of TEE-guided interventional occlusion for treating congenital heart disease (CHD). Methods: Clinical data of 50 CHD patients who underwent TEE-guided minimally invasive closure were analyzed retrospectively. There were 29 cases of ventricular septal defect (VSD), 12 cases of atrial septal defect (ASD), 1 case of ASD combined with VSD and 8 cases of patent ductus arteriosus (PDA). The interventional occlusions were performed through transthoracic approach. Before occlusion, TEE was used to diagnose and observe the heart defect again, while intraoperative TEE was used for real-time monitoring and guiding the wire and sheath aimed to the designated position and occlusion umbrella placement. Postoperative TEE was also performed to evaluate the occlusion effect. Electrocardiogram was used to monitor the arrhythmia and conduction block of patients. Results: Interventional occlusions were successfully performed in 49 cases under the guidance of TEE. Remaining shunts were found in 11 patients with TEE immediately after interventional occlusion. TEE reexamined at 1, 3 and 6 months after operation showed satisfied occluder position in all 49 patients. Neither residual shunt nor valve regurgitation caused by occlusion was found. And there was no arrhythmia and conduction block detected with ECG. The occlusion of VSD failed in 1 patient. Conclusion: TEE is a safe, effective, minimally invasive, radiation-free and needing no contrast media method for guiding transthoracic interventional occlusion for treatment of CHD, worthy of clinical popularization and application.
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Objective To study the the treatment effects of Niuhuang-Xingnao pill on Alzheimer's diseases mice and its influence on the levels of Bcl-2, caspase-3 and C-caspase-3. Methods 50 APP/V717 mice were divided into the positive control group, the model group,Niuhuang-Xingnao pill high, medium and low dosage groups. 10 C57BL/6 mice were selected as blank control. The high, medium and low dosage groups were given 142, 71 and 35.5 mg/kgNiuhuang-Xingnao pill, the positive control group was given 10 mg/kg donepezil, the blank group and model group were given same volume normal salin. 1 time/day, and continuous administration for 60 d. Morris water maze experiment was performed to test the learning and memory ability in mice. The levels of CAT, SOD and GSH-Px were detected by the method of ELISA, while Bcl-2, caspase-3 and C-caspase-3 in the groups were detected by western-blotting. The apoptosis of hippocampus of mice were observed by the method of TUNEL. Results Compared with the model group, the third square time (36.58 ± 4.57 s, 32.46 ± 4.25 s, 29.71 ± 4.26 s vs.25.48 ± 3.91 s) of high, middle and low dose groups were significantly longer, cross time of the table (5.82 ± 0.87, 4.59 ± 0.76, 3.96 ± 0.75 vs.3.27 ± 0.53) were significantly higher (P<0.05). In the high, middle and low dose groups, the levels of GSH-Px (161.14 ± 14.01 U/mg, 150.76 ± 13.16 U/mg, 143.17 ± 12.54 U/mg vs. 120.78 ± 10.92 U/mg), SOD (14.25 ± 1.82 U/mg, 11.17 ± 1.65 U/mg, 7.24 ± 1.02 U/mg vs. 3.12 ± 0.31 U/mg), CAT (17.95 ± 2.16 U/mg, 16.72 ± 1.83 U/mg, 15.54 ± 1.47 U/mg vs. 13.25 ± 2.60 U/mg) were significantly higher (P<0.05);caspase-3 (1.13 ± 0.13, 1.25 ± 0.15, 1.41 ± 0.17 vs. 1.49 ± 0.20), C-caspase-3 (1.17 ± 0.14, 1.27 ± 0.16, 1.42 ± 0.18 vs.1.52 ± 0.23) significantly lower(P<0.05), Bcl-2 (0.88 ± 0.08, 0.79 ± 0.06, 0.67 ± 0.04vs. 0.59 ± 0.04) significantly higher(P<0.05).ConclusionsNiuhuang-Xingnao pill treatment of Alzheimer's disease in mice can effectively promote the expression of Bcl-2, caspase-3 and C-caspase-3 in the hippocampus of mice, inhibit the apoptosis of the cells in hippocampus.
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Objective To study the the treatment effects of Niuhuang-Xingnao pill on Alzheimer's diseases mice and its influence on the levels of Bcl-2, caspase-3 and C-caspase-3. Methods 50 APP/V717 mice were divided into the positive control group, the model group,Niuhuang-Xingnao pill high, medium and low dosage groups. 10 C57BL/6 mice were selected as blank control. The high, medium and low dosage groups were given 142, 71 and 35.5 mg/kgNiuhuang-Xingnao pill, the positive control group was given 10 mg/kg donepezil, the blank group and model group were given same volume normal salin. 1 time/day, and continuous administration for 60 d. Morris water maze experiment was performed to test the learning and memory ability in mice. The levels of CAT, SOD and GSH-Px were detected by the method of ELISA, while Bcl-2, caspase-3 and C-caspase-3 in the groups were detected by western-blotting. The apoptosis of hippocampus of mice were observed by the method of TUNEL. Results Compared with the model group, the third square time (36.58 ± 4.57 s, 32.46 ± 4.25 s, 29.71 ± 4.26 s vs.25.48 ± 3.91 s) of high, middle and low dose groups were significantly longer, cross time of the table (5.82 ± 0.87, 4.59 ± 0.76, 3.96 ± 0.75 vs.3.27 ± 0.53) were significantly higher (P<0.05). In the high, middle and low dose groups, the levels of GSH-Px (161.14 ± 14.01 U/mg, 150.76 ± 13.16 U/mg, 143.17 ± 12.54 U/mg vs. 120.78 ± 10.92 U/mg), SOD (14.25 ± 1.82 U/mg, 11.17 ± 1.65 U/mg, 7.24 ± 1.02 U/mg vs. 3.12 ± 0.31 U/mg), CAT (17.95 ± 2.16 U/mg, 16.72 ± 1.83 U/mg, 15.54 ± 1.47 U/mg vs. 13.25 ± 2.60 U/mg) were significantly higher (P<0.05);caspase-3 (1.13 ± 0.13, 1.25 ± 0.15, 1.41 ± 0.17 vs. 1.49 ± 0.20), C-caspase-3 (1.17 ± 0.14, 1.27 ± 0.16, 1.42 ± 0.18 vs.1.52 ± 0.23) significantly lower(P<0.05), Bcl-2 (0.88 ± 0.08, 0.79 ± 0.06, 0.67 ± 0.04vs. 0.59 ± 0.04) significantly higher(P<0.05).ConclusionsNiuhuang-Xingnao pill treatment of Alzheimer's disease in mice can effectively promote the expression of Bcl-2, caspase-3 and C-caspase-3 in the hippocampus of mice, inhibit the apoptosis of the cells in hippocampus.