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Article de Chinois | WPRIM | ID: wpr-1038870

RÉSUMÉ

Objective @#To investigate the effect of paeoniflorin (PF) on liver injury mediated by NLRP3 inflamma- some pathway in db / db mice.@*Methods @#In this study,db / db mice were used as the mice model of type 2 diabetic while db / m mice were used as control group.The mice were randomly divided into six groups : db / m group,db / m + PF group,db / db group,db / db + PF 25 mg / kg group ,db / db + PF 50 mg / kg group,db / db + PF 100 mg / kg group.After 12 weeks of PF gavage,mouse serum samples were collected to detect the levels of alanine aminotrans- ferase(ALT) ,aspartate aminotransferase(AST) ,total cholesterol(TC) ,triglyceride(TG) and free fat acid(FFA) . HE staining,oil red O staining and Sirius red staining were used to observe the degree of pathological injury of liv- er.The expression of F4 /80,α-SMA and Col Ⅲ protein was detected by immunohistochemistry.The expression of interleukin-1 β ( IL-1 β ) , interleukin-18 ( IL-18 ) , tumor necrosis factor-α ( TNF-α ) , NOD-like receptor 3 (NLRP3) ,apotpsis associated spck-like protein ( ASC) and cysteinyl asparate specific proteinase-1 ( Caspase-1 ) was detected by Western blot. @*Results @#Compared with db / m group,the levels of serum ALT,AST,TC,TG and FFA increased in db / db group,and these indexes decreased after PF gavage. Compared with db / m group,his- topathological examination of the liver revealed increased hepatic tissue lipid accumulation,inflammatory cell infil- tration,and collagen deposition in db / db mice,and PF treatment reduced hepatic lipid accumulation,inflammatory cell infiltration,and collagen deposition.Meanwhile,compared with db / m group,the expression of proinflammato- ry cytokines (IL-1 β , IL-18 and TNF-α) ,F4 /80,α-SMA,Col Ⅲ , NLRP3,ASC and Caspase-1 protein in the liver of db / db mice increased,and the expression of these proteins decreased after gavage with PF.@*Conclusion@#PF inhibited the NLRP3 inflammatory pathway and attenuated liver inflammation and fibrosis in db / db mice.

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