Clinical value of 18 F-FDG PET-CT in aiding CT-guided percutaneous lung biopsy / 实用放射学杂志

Objective To investigate the clinical value of PET-CT in aiding CT guided percutaneous puncture biopsy for lung mas-ses.Methods The retrospective data were analyzed including 5 7 patients with lung masses who underwent CT guided percutaneous puncture biopsy combined with PET-CT (regarded as a research group)and other 75 similar patients with single CT-guided biopsy (control group).In two groups,it was divided with malignant,suspicious malignant,can not be diagnosed,inflammatory,benign by pathological findings,and was contrasted with the surgical pathology or final clinical results.To calculate sensitivity,specificity and accuracy of diagnosis for cancer through two puncture method.Recorded complication for pneumothorax and bleeding in two groups .Be compared analysis using the 2 test.Results In study group,it was higher for the diagnosis of malignancy sensitivity, specificity,accuracy.There were 96.3% (52/54),100.0%(3/3)and 96.5%(55/57)in research group,while those were 88.4%(61/69),83.3% (5/6)and 88.0% (66/75)in the control group,respectively.And no statistically significant.Between research and the control group,there was a significantly statistical difference in the rate of twice punctures in one mass (χ2= 10.122,P=0.001).Between the two groups,there was no statistically significant difference in the pneumothorax rate and the hemorrhage rate. Conclusion PET-CT in aiding CT-guided lung biopsy improves the sensitivity,specificity and accuracy of diagnosis.

The study on the characters of membrane protein interaction and its network based on integrated intelligence method / 生物医学工程学杂志

Membrane protein and its interaction network have become a novel research direction in bioinformatics. In this paper, a novel membrane protein interaction network simulator is proposed for system biology studies by integrated intelligence method including spectrum analysis, fuzzy K-Nearest Neighbor(KNN) algorithm and so on. We consider biological system as a set of active computational components interacting with each other and with the external environment. Then we can use the network simulator to construct membrane protein interaction networks. Based on the proposed approach, we found that the membrane protein interaction network almost has some dynamic and collective characteristics, such as small-world network, scale free distributing, and hierarchical module structure. These properties are similar to those of other extensively studied protein interaction networks. The present studies on the characteristics of the membrane protein interaction network will be valuable for its relatively biological and medical studies.

Prediction of G-protein-coupled receptor classes with pseudo amino acid composition / 生物医学工程学杂志

G-protein-coupled receptors (GPCRs), the largest family of cell surface receptors, play an important role in the production of therapeutic drugs. The functions of GPCRs are closely related to their classification and subclassification. It is difficult to obtain the spatial structure of GPCRs sequence by experimental approaches. It is highly desired to develop powerful tools and effective algorithms for classifying the family of GPCRs. In this study, based on the concept of pseudo amino acid composition (PseAA) originally introduced by Chou, approximate entropy (ApEn) of protein sequence as an additional characteristic is used to construct PseAA. A 21-D (dimensional) PseAA is formulated to represent the sample of a protein. Fuzzy K nearest neighbors (FKNN) classifier is adopted as prediction engine. The datasets in low homology are used to validate the performance of the proposed method. Compared with prior works, the successful rates achieved of our research are the highest. The test results indicate that the novel approach can play the role of a compliment to many of the existing methods, which promises to be a useful tool for GPCRs function prediction.

Protein structural class prediction with binary tree-based support vector machines / 生物医学工程学杂志

A new mutil-classification method based on binary tree SVM (BT-SVM) is presented to predict protein structural class. The protein sequence, which is represented by 26-D vector, is used as input vector. BT-SVM method resolves unclassifiable regions for multiclass problems which can not be solved by SVM. Self-consistency and cross validation test are used to verify the performance of the proposal method on two benchmark datasets. Satisfactory test results demonstrate that the new method is promising. The Jackknife results of the new method are compared with the existing results on the same datasets. The results of the new method are almost the same as the ones of the best exiting method. It illuminates that the new method has good prediction performance and it will become a useful tool in protein structure class prediction.

Protein secondary structure prediction based on maximum entropy model / 生物医学工程学杂志

Secondary structure prediction plays an important role in function prediction of protein. In this paper, maximum entropy model is used to predict protein secondary structure. We build feature function sets based on the influential factors which are crucial to the states of secondary structure of residues in protein sequence. Multi-factors are taken into account in the model, including charge of amino acids, conformational parameter for the states of secondary structure, short and long ranges of interaction of residues in sequence. As such, multi-source information is integrated into a single probability model by the method. Compared with the reported methods, our method gets a higher accuracy rate in predicting protein secondary structure. The results demonstrate that the proposed method is practical.

CT Analysis of the Lumbar Degenerative Spondylolisthesis(A Report of 125 Cases) / 实用放射学杂志

Objective To explore the etiology of lumbar degenerative spondylolisthesis(DS)by analyzing its CT manifestations.Methods The location,degree and angle of inclination of lumbar facet joints were measured in 125 cases with 131 spondylolisthesis.Results The angle of inclination of lumbar facet joints was

CT Diagnosis for Peritoneum Metastatic Tumor / 实用放射学杂志

Objective To investigate characteristic of CT findings for peritoneal metastases.Methods The CT manitestations of 48 cases with pathologically proved peritoneal metastases were analysed. Results ①Ascites was seen in 32 cases, to make up 66.7%. ②Changes of omentum were found in 16 cases, to make up 33.3%; In it, cake sign of omentum were in 8 cases, nodular sign of omentum were in 5 cases, cystic sign of omentum were in 2 cases, opacity sign of omentum was in 1 case. ③Changes of mesentery were in 14 cases, to make up 29.2%. ④Thickening of the parieral peritoneurmar were in 12 cases, to make up 25%. ⑤Thickening of small intestine wall and moving of intestine were in 2 cases, to make up 4.2%.Conclusion CT is a valuable method for the diagnosis of peritoneum metastatic tumor.

MR Diagnosis of Extrahepatic Biliary Obstructive Disease / 实用放射学杂志

Objective To study MR manifestations of biliary obstructive disease.Methods MR examination (Philips Gyroscan INTERA 1.0T)in 69 patients with biliary obstructive disease were performed.The conventional sequences included:T1WI/FFE,T2WI/TSE,T2WI/SPIR and contrast-enhanced at axial position,coronal B-FFE and MRCP.All images in all cases were compared with the results of operation and pathology.Results 43 cases were benign disorder,of them,42 cases were choledocholithiasis,one case was choledochal cyst.Choledocholithiasis presented as single or multiple round or ovoid with low signal.The direct sign of malignant lesions (26 cases) was the masses with slightly high signal at the obstructive part,the indirect sign of malignant lesions was obstruction or narrow of bile duct. The MR diagnostic accuracy of obstructive location was 100%, and the accuracy for evaluating the causes of obstruction was 89.9%.Conclusion MRCP combining with B-FFE and routine MRI, analysising the direct sign and indirect sign, can improve the diagnostic accuracy of obstructive jaundice.

The Value Of MRI in Diagnosis of Spinal Metastasis:An Analysis of 85 Cases / 实用放射学杂志

Objective To study the value of MRI in the diagnosis of spinal metastasis.Methods MR imaging manifestations of spinal metastasis in 85 cases were retrospectively analyzed.46 cases underwent CT and/or X-ray in the 85 cases.Results Totally 388 metastatic vertebra in the 85 cases were detected by MRI.388 vertebras showed hypointensity on T1WI.354 vertebra showed mixed signal intensity and 34 vertebra showed hypointensity on T2WI.All of the vertebra showed hyperintensity on T2WI/STIR.54 vertebra showed pathologic fracture,53 vertebra were protrusion in postetrior,54 vertebra with appendages damage and paravertebral soft tissue mass presented and vertebral canal involved in 30 cases.18 cases were detected with lesion in 46 cases having X-ray and/or CT data,the positive rate was 39.11%(18/46).Conclusion MRI can detect spinal metastatic lesion early,the extent and degree of the tumors can be judged by MRI exactly.

MRI Diagnosis of Small Epidermoids in the Cerebellopontine Angle:Preliminary Study / 实用放射学杂志

Objective To investigate the best MR technique for detecting small epidermoids in the cerebellopontine angle.Methods 7 patients with pathologically-proved small epidermoids only located in the cerebellopontine angle were collected. The imaging techniques included:conventional T1WI and T2WI, fluid attenuated inversion recovery(FLAIR),diffusion weighted imaging(DWI) and balanced fast field echo(B-FFE) .The findings were retrospectively analyzed.Results 6 of 7 cases of epidermoid tumours localized at the cerebellopontine angle and exhibited the similar signal intensity as the cerebrospinal fluid(CSF) on T1WI and T2WI.The remaining case was slightly isointense on either T1WI or T2WI.On FLAIR images in 6 cases,3 cases showed slightly hyperintensity, 1 case showed doubtful heterogeneous signal intensity and 2 cases did not reveal any abnormal signal intensity.3 cases were hyperintense differenting from CSF on DW images. All of seven cases showed heterogeneous signal characteristics and were quite different from CFS on B-FFE.Meanwhile B-FFE also produced clearly the tissue contrast of trigeminus embedded in epidermoids of the cerebellopontine angle.Conclusion B-FFE is much more useful for the early diagnosis of epidermoids in the cerebellopontine angle and it also can provide precise preoperative information about the trigeminus encasement.