Observation of the efficacy of endoscopic myringoplasty with homoplastic amniotic membrane / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To explore the clinical effectiveness of endoscopic myringoplasty with homoplastic amniotic membrane.@*METHOD@#A retrospective study in 43 patients underwent endoscopic myringoplasty with homoplastic amniotic membrane was observed at the wound healing of tympanic membrane perforation and the hearing improve-threshold audiometry in 43 patients after surgery.@*RESULT@#The tympanic membrane's perforation healing rate was 95.3% (41/43), and air conduction threshold audiometry in language frequency improved average 10 dB. The follow up was 1--4 years, and no recurrence or complications had occurred.@*CONCLUSION@#The endoscopic myringoplasty could be able to simple myringoplasty whose air conduction threshold audiometry in language frequency was less than 40 dB. It could be used to repair not only the central perforation in tympanic membrane, but also the marginal perforation which has residual tympanic membrane in most of the regional. Human amniotic membrane is an ideal materials for repair of tympanic membrane.

Clinical study of 26 patients with osteoma in the external / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To summarize the experience of diagnosis and treatment on osteoma in the external auditory canal.@*METHOD@#Retrospective study were undertaken in 26 patients with osteoma in the external auditory canal operated in author's unit.@*RESULT@#Osteomas were removed through interauricular approach in all patients and confirmed by histopathological examination. The hearing of 22 patients with conductive hearing loss became normal after operation. The air conduction hearing threshold in 2 patients with mixed hearing loss improved average 15 dB and 20 dB respectively after operation. The follow up was more than 1 year, and no recurrence or complications had occurred.@*CONCLUSION@#Osteoma in the external auditory canal is an uncommon benign lesion. The method of choice in diagnosis is temporal bone CT scan. Osteoma must be distinguished from exostosis in the external auditory canal. Osteoma is confirmed by pathological diagnosis and surgery is the only method for treatment.

Effect of anteroposterior cricoid split on cartilage growth in rabbits / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate the effect of the anteroposterior cricoid split on cartilage growth.@*METHOD@#The rabbits were killed 8 months after the surgery of anteroposterior cricoid split. The larynxes were harvested and the cross-sectional area of the cricoid cartilage were calculated.@*RESULT@#There was no significant difference in the cross-sectional area of the cricoid cartilage of these three groups.@*CONCLUSION@#The anteroposterior cricoid split might have no adverse effect on subsequent growth of cricoid cartilage.

The diagnostic value of electronystagmography in vertigo in elderly patients with vertebro-basilar insufficiency / 解放军医学杂志

Objective To explore the electronystagmography (ENG) changes and their clinical significance in aged patients with vertebro-basilar insufficiency (VBI). Methods Sixty aged patients with VBI were selected as experimental group for testing of visuo-oculomotor system reaction, spontaneous nystagmus, caloric test and positional nystagmus. Forty normal aged persons as control group. Results In the experimental group, there were 11 cases (18.3%) who had spontaneous nystagmus and 46 cases (76.6%) with positional nystagmus. The positional nystagmus intension in those patients was 7.76?6.05?/s, which was much higher compared to the control group (P

Exogenous WAF_1-S gene inhibits growth of laryngeal cancer Hep-2 cell line / 解放军医学杂志

Objective To investigate the inhibitory effects of exogenous WAF 1 S gene on human laryngeal cancer Hep 2 cell line, and to explore the potential use of WAF 1 S in gene therapy for laryngeal cancer. Methods A eukaryotic expression vector containing 2 1kb human full length WAF 1 S cDNA was transfected into human laryngeal cancer Hep 2 cell line by using lipofectamine. Expression of exogenous WAF 1 S gene was detected by dot blot hybridization. By using Western blot and confocal microscope, expression of p21 protein was quantitatively analyzed in situ . The growth state of transfected Hep 2 cell was determined by flow cytometry and MTT. Results It was found by dot blot hybridization that WAF 1 S gene could express in Hep 2 cell. The expression of the exogenous p21 gene in Hep 2 cells was markedly higher than that in the control group. It was confirmed with flow cytometry that WAF 1 S gene could induce apoptosis of laryngeal cancer Hep 2 cell line, and the progression of cell cycle was arrested at G 1 phase. Conclusion Laryngeal cancer cells could be arrested at G 1 /S phase and the growth of the cells could be significantly suppressed by exogenous WAF 1 S gene