27-Hydroxycholesterol modulates lung cancer cell proliferation by activation of LXR signaling pathway / 中国病理生理杂志

AIM:To investigate the effect of cholesterol metabolite 27-hydroxycholesterol (27-OHC) on the proliferation of lung cancer cells.METHODS:Human lung cancer A549 cells were treated with 27-OHC at different concentrations (0, 0.3125, 0.625, 1.25, 2.5, 5 and 10μmol/L) for 24～48 h.The cell viability, cell cycle, cell proliferation, the intracellular cholesterol levels and cholesterol metabolism-related molecule expression were subsequently assessed by CCK-8 assay, flow cytometry, Ed U staining, tissue total cholesterol detection kit, real-time PCR and Western blot.RESULTS:27-OHC decreased the viability of the A549 cells in a dose-and time-dependent manner (P<0.01) and inhibited the cell proliferation (P<0.05).The expression of typical liver X receptor (LXR) downstream target proteins including ATP-binding cassette transporter A1 (ABCA1) , low-density lipoprotein receptor (LDLR) , and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CR) were modulated, which promoted the efflux of intracellular cholesterol, and reduced cholesterol influx and de novo synthesis, resulting in decreased intracellular cholesterol levels and cell viability.Furthermore, the inhibitory effect of 27-OHC on A549 cell viability was significantly attenuated after the LXR pathway was partially blocked by 5μmol/L GSK2033 treatment (P<0.05).CONCLUSION:27-OHC inhibits A549 cell proliferation via activation of LXR signaling pathway.

The early evaluation of lowGdose CT perfusion imaging for the treatment efficacy of lung cancer with anrotinib hydrochloride / 实用放射学杂志

Objective To explore whether CT perfusion imaging (CTPI)parameters can early predict the curative effect of anlotinib hydrochloride and their predictive accuracy for the treatment in lung cancer patients.Methods 2 6 patients with advanced nonGsmall cell lung cancer (NSCLC)were treated with anlotinib hydrochloride and underwent CTPI scanning before chemotherapy,after the first and second treatment cycle respectively.The average values of perfusion value (PV),peak enhancement image (PEI),time to peak (TTP),blood volume (BV)and the change rate of these parameters after one treatment cycle every time were measured and recorded. According to the response evaluation criteria in solid tumors 1.1 (RECIST1.1),the maximum diameter of the target tumor was measured and the tumor regression rate after two treatment cycles was calculated.Then a correlation analysis was conducted between the change rate of perfusion parameters (PV%,PEI%,TTP%,BV%)after one treatment cycle and the tumor regression rate (D%)after two treatment cycles. The ROC curve was performed to evaluate the accuracy of those parameters.Results PV after one treatment cycle was significantly lower than that before treatment,and PV% showed a statistical difference (P＝0.00).The PV% after one treatment cycle was positively correlated with D% after two treatment cycles (r＝0.56).In addition,the AUC of PV% and BV% were 0.99 and 0.88 respectively, and specificity were both 100%,with sensitivity respectively 75.7% and 82.6%.Conclusion CTPI can early reflect the curative effect of anlotinib hydrochloride for advanced NSCLC and provide more options for clinical evaluation.

Diagnostic value of high field intensity MRI in early changes of wrist RA / 中国医学装备

Objective: To explore the diagnostic value of high field intensity magnetic resonance imaging(MRI) in the early changes of wrist rheumatoid arthritis(RA). Methods: 150 patients with joint pain were enrolled in the retrospective analysis. And in these patients, the patients with RA who were diagnosed by using high field intensity MRI were divided into observation group(102 cases), and others were divided into control group (48 cases). The diagnostic value of high field intensity MRI for the early change of wrist RA were further explored and discussed. Results: The diagnostic sensitivities of early change, synovial thickening, bone marrow edema, bone erosion, arthroedema and myotenositis of high field intensity MRI for RA were higher than 60%, and the diagnostic specificity of them were higher than 50%, and the diagnostic sensitivity of synovial thickening of these indicators even achieved to 100%. The positive rates of these indicators of observation group was significantly higher than that of control group, respectively (x2=108.147, x2=27.943, x2=50.473, x2=51.549, x2=17.558, P<0.05). And all of synovial thickening, bone marrow edema, bone erosion, arthroedema and myotenositis were the independent influence factors of early diagnosis of wrist RA(OR=9.631, OR=10.114, OR=25.867, OR=26.258, OR=14.027, P<0.05). Conclusion: MRI is one of the reliable methods for diagnosing early wrist RA. Synovial thickening, bone marrow edema, bone erosion, joint effusion and tendonitis can be used as the main symptom in diagnosis. And they can be combined with clinical symptom, sign and laboratory examination results to carry out comprehensive judgment for RA of wrist.

Neuroprotective effect of intracarotid cold saline infusion following cardiopulmonary resuscitation in a hypothermic cardiac arrest canine model / 解放军医学杂志

Objective To evaluate the effect of intracarotid cold saline infusion (ICSI) on neurological outcomes in canines with cardiac arrest (CA) introduced by severe hypothermia.Methods Restoration of spontaneous circulation (ROSC) after hypothermic CA was induced in 10 Beagle dogs.These 10 dogs were randomly divided into 2 groups (5 each).Dogs in control group were rewarmed using warn water bath,and dogs in experimental group received the bath rewarming plus ICSI for 6 hours to maintain the brain temperature ＜36℃.In both groups,the Neurologic Disability Scores (NDS) were recorded at 24h after the ROSC,and their brains were removed for pathologic analysis using hematoxylin and eosin stain.The brain water content and sl00β of serum level were also measured.Results The water content (79.43％ ± 0.72％ vs.80.79％ ± 1.06％,P＜0.05) and serum level of s100β (119.83 ± 42.93pg/ml vs.329.82 ± 190.39pg/ml,P＜0.05) were significantly lower in experimental group than in the control group.Control group presented obvious pathological damage of the hippocampal pyramidal cells.There was no significant difference in NDS between the two groups.Conclusion ICSI could reduce the production of s100β and pathological brain damage in postarrest hypothermic canines.

Type Distribution of Unexpected Red Cell Antibodies in Patients with Malignancy / 대한수혈학회지

BACKGROUND: Performance of antibody screening and identification tests before blood transfusion is important because the unexpected presence of red cell antibodies may cause hemolytic transfusion reactions. Many patients with malignancy undergo transfusion in order to overcome pancytopenia due to disease itself or chemotherapy. We investigated the type distribution of unexpected red cell antibodies in cancer patients and compared our results with those of other institutions. METHODS: From January 2008 to June 2011, 30,989 serum samples were screened using a LISS/Coombs card and ID-DiaCell I, II (DiaMed AG, Morat, Switzerland). Data-Cyte Plus Reagent Red Blood Cells (Medion Diagnostics, Dudingen, Switzerland) were used in performance of antibody identification tests. RESULTS: Out of 30,989 serum samples, 180 cases (0.58%) showed screening-positive results, and unexpected antibodies were identified in 72 cases. The type of unexpected antibody observed most often in cancer patients was a member of the Rh antibody group, anti-E in 17 cases (29.8%), followed by anti-Lea in five cases (8.8%) and anti-e in three cases (5.3%). While Rh group antibodies were observed in the colon cancer group, non-Rh group antibodies were observed in the rectal cancer group. And, in the genitourinary cancer group, Lewis group antibodies were more frequently detected than others. CONCLUSION: Findings from our study demonstrated a type distribution of unexpected red cell antibodies that was similar to those reported in previous studies. Compared with non-cancerous patients, no difference in type distribution of unexpected red cell antibodies was observed in cancer patients. Some antibodies were frequently observed in certain cancer groups. Further comprehensive research on unexpected antibodies based on location or histologic type of cancer is needed.