Studies on chemical constituents of rhizome of Matteuccia struthiopteris / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of the rhizome of Matteuccia struthiopteris.</p><p><b>METHOD</b>The constituents were separated and purified by column chromatography with silica gel and Sephadex LH-20. Their structures were identified on the basis of physical and spectral data.</p><p><b>RESULT</b>Six compounds were isolated and identified as demethoxymatteucinol (1), matteucinol (2), pinosylvin (3), matteuorien (4), pinosylvin 3-O-beta-D-glucopyranoside (5), matteuorienate A (6).</p><p><b>CONCLUSION</b>All Compounds were isolated from this plant for the first time.</p>

Determination of artemisinin, arteannuin B and artemisinic acid in Herba Artemisiae Annuae by HPLC-UV-ELSD / 药学学报

To establish an HPLC-UV-ELSD method for the determination of the content of artemisinin, arteannuin B and artemisinic acid in Herba Artemisiae Annuae. The analytical column was Nucleodur RP-C18 (250 mm x 4.6 mm, 5 microm ID). The mobile phase was acetonirile-0.1% acetic acid (50: 50) and the flow rate was 1.0 mL x min(-1) with a UV detector for artemisinin, the detection wavelength at 209 nm, and the evaporative light-scattering detector (ELSD) for arteannuin B and artemisinic acid, the drift tube temperature: 50 degrees C, the nitrogen flow rate 30 psi and the gain was 50. The resolution of artemisinin, arteannuin B and artemisinic acid was good. The linear calibration curves were obtained over the range of 0.52 - 2.6 microg for artemisinin (r = 0.999 4, n = 5), 0.022 - 4.4 microg for artemisinin B (r = 0.999 9, n = 5) and 0.203 - 8.12 microg for artemisinic acid (r = 0.999 8, n = 5), separately. The mean recoveries of the three compounds were 99.45%, 102.37% and 101.10% with RSD of 2.3%, 1.7% and 0.79%, respectively. This method is simple, rapid, accurate and suitable for the determination of the content of the three compounds in the herbs.

Studies on the chemical constituents from stem of Chirita longgangensis var. Hongyao / 中国中药杂志

<p><b>OBJECTIVE</b>To isolate and elucidate the constituents from stem of Chirita longgangensis var. hongyao.</p><p><b>METHOD</b>The constituents were extracted with methanol and isolated by chromatography on silica gel, Sephadex LH-20 and ODS. The structures were determined by NMR and MS spectral analysis.</p><p><b>RESULT</b>Seven compounds were identified as 2-hydroxy-7-methyl-9, 10-anthraquinone (1), 2-methyl-9, 10-anthraquinone (tectoquinone) (2), ursolic acid (3), vanillic acid (4), beta-sitosteryl-D-glucoside-6'-palmitate (5), beta-sitosterol (6), daucosterol (7), respectively.</p><p><b>CONCLUSION</b>Compounds 1, 2, 3 and 5 were isolated for the first time from the family Gesneriaceae, compounds 4 and 6 were isolated for the first time from the genus Chirita, and compound 7 was isolated for the frist time from Chirita longgangensis var.</p>

Chemical constituents of the rhizome of Matteuccia struthiopteris / 药学学报

<p><b>AIM</b>To study the chemical constituents of the rhizome of Matteuccia struthiopteris (L.) The constituents were separated and purified by column chromatography with normal Todaro.</p><p><b>METHODS</b>phase silica gel and Sephadex LH-20. Their structures were identified on the basis of physical and spectral data (MS, NMR, HMBC and HMQC).</p><p><b>RESULTS</b>Four compounds were isolated and identified as 1-O-beta-D-gl ucopyranosyl-(2S,3R,4E, 8Z) -2-N-(2'-hydroxydocosanoyl) eicosasphinga-4,8-dienine (1), 1-O-beta-D-galactosyl-(6-->1)-alpha-D-galactosyl-2,3-O-dihexadecanoyl-glycerol (2), succinic acid (3), D-mannitol (4).</p><p><b>CONCLUSION</b>Compounds 1 and 2 are new compounds. Compounds 3 and 4 were isolated from this plant for the first time.</p>

Phenylethanoid glycosides from stem of Chirita longgangensis var. hongyao / 中国中药杂志

<p><b>OBJECTIVE</b>To isolate and elucidate the constituents from stem of Chirita longgangensis var. hongyao.</p><p><b>METHOD</b>The constituents were extracted with methanol and isolated by chromatography on silica gel, Sephadex LH-20 and ODS. The structures were determined by NMR and MS spectral analysis.</p><p><b>RESULT</b>Five phenylethanoid glycosides were identified as 3, 4-dihydroxyphenyl alcohol-beta-D-glucopyranoside (1), 3, 4-dihydroxyphenyl alcohol-6-O-caffeoyl--D-glucopyranoside (calceolarioside B) (2), 3, 4-dihydroxyphenyl alcohol-beta-D-glucopyranosyl-(1 --> 3)-6-O-caffeoyl-beta-D-glucopyranoside (plantainoside D) (3), 3, 4-dihydroxyphenyl alcohol-beta-D-glucopyranosyl-(1 --> 3)-4-O-caffeoyl-beta-D-glucopyranoside (plantamajoside) (4), 3, 4-dihydroxyphenyl alcohol-beta-D-glucopyranosyl-(1 --> 3)-6-O-feruloyl-beta-D-glucopyranoside (scroside E) (5), respectively.</p><p><b>CONCLUSION</b>Compounds 3 and 5 were isolated for the first time from the family Gesneriaceae, and compounds 1, 2 and 4 were isolated for the first time from the genus Chirita.</p>

Studies on chemical constituents in rhizome of Matteuccia struthiopteris / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents in rhizome of Matteuccia struthiopteris.</p><p><b>METHOD</b>The compounds were isolated by normal phase silica gel chromatography. The structures were identified by physical and spectral data.</p><p><b>RESULT</b>Six compounds were isolated and identified as woodwardic acid (1), ergost-6,22-diene-3beta,5alpha,8alpha-triol (2), apigenin (3), riboflavin (4), 4-O-beta-D-glucopyranosyl-p-coumaric acid (5), 4-O-beta-D-glucopyranosylcaffeic acid (6).</p><p><b>CONCLUSION</b>All the compounds were obtained from this plant for the first time.</p>

Effect of dihydro-qinghaosu on auto-antibody production, TNF alpha secretion and pathologic change of lupus nephritis in BXSB mice / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To investigate the effect of dihydro-qinghaosu (DQHS) on auto-antibody production, TNF alpha secretion and pathologic change of lupus nephritis in BXSB mice and the possible mechanism of DQHS in treating systemic lupus erythematosus (SLE).</p><p><b>METHODS</b>Anti ds-DNA antibody and TNF alpha in serum of the BXSB mice were detected by enzyme linked immunosorbent assay (ELISA). Renal tissue was stained by HE and Masson.</p><p><b>RESULTS</b>In the height and moderate dose DQHS groups, as compared with the model group, levels of anti-ds-DNA antibody and serum TNF alpha were significantly lower (P < 0.05); and renal pathological change was milder.</p><p><b>CONCLUSION</b>DQHS could inhibit the production of anti-ds-DNA antibody and secretion of TNF alpha and improve the pathologic lesion of lupus nephritis in BXSB mice.</p>