Simultaneous determination of eleven flavonoid glycosides in ginkgo biloba leaves collected in different seasons by UPLC PDA method / 药学学报

A new UPLC method was developed for the simultaneous determination of eleven characteristic flavonoid glycosides in Ginkgo biloba leaves. The natural occurrence of flavonoid glycosides in Ginkgo biloba leaves within one vegetative season was investigated for the first time. The analysis was performed on an Agilent ZORBAX Eclipse Plus C18 column (50 mm x 4.6 mm, 1.8 microm), the mobile phase A was acetonitrile, the mobile phase B was 0.4% phosphate aqueous solution in a gradient elution at a flow rate of 0.6 mL x min(-1), the detection was carried out at 360 nm. The result showed that eleven flavonoid glycosides had good linearity with good average recovery, separately. The method was proved to be accurate, rapid and good reproducible for the quality evaluation of Ginkgo biloba leaves, and provide an easy and rapid means for the quantitative analysis of flavonoid glycosides and their content fluctuation with seasons.

Determination of Camphor, Borneol and Bornyl Acetate in Shenling Baizhu Powder by Gas Chromatography / 中国中医药信息杂志

Objective To establish a GC method for simultaneous determine of camphor, borneol and bornyl acetate in Shenling Baizhu Powder. Methods Capillary column was used with 100% methyl polysiloxane as stationary phase. The temperature-programmed was as follows:starting temperature of 60 ℃, 5 ℃/min, rose to 130 ℃, kept for 5 minutes, followed by 20 ℃/min up to 230 ℃, and kept for 5 minutes. Results The average recovery rate of camphor was 99.87%, RSD was 1.3%, and good linear relationship was showed in the range of 0.003 77-0.150 8 μg (r=0.999 9). The average recovery of borneol was 100.71%, RSD was 2.1%, and good linear relationship was showed in the range of 0.002 411-0.096 4 μg (r=0.999 9). The average recovery rate of bornyl acetate was 101.95%, RSD was 1.3%, and good linear relationship was showed in the range of 0.003 02-0.121 1 μg (r=0.999 9). Conclusion The method is simple, reliable, accurate, and can effectively control the quality of Amomi Fructus in Shenling Baizhu Powder.

Determination of main free amino acids in Banlangen Keli by UPLC / 中国中药杂志

<p><b>OBJECTIVE</b>To establish a quantitative method with precolumn derivatization for determining the contents of six common amino acids in Banlangen Keli by UPLC.</p><p><b>METHOD</b>Using 6-acetamido-4-hydroxy-2-methyl quinoline as the derivating agent, we determined the contents of arginine, threonine , alanine, gamma aminobutyric acid, proline, and valine. The UPLC analysis was performed on a Waters AccQ Tag TM Ultra C18 column (2.1 mm x 100 mm, 5 microm) with mobile phase AccQ Tag Ultra Eluent A and AccQ Tag Ultra Eluent B gradient elution at a flow rate of 0.7 mL x min(-1). The column temperature was 55 degrees C and detection wavelength was 260 nm.</p><p><b>RESULT</b>The linear ranges of arginine, thremine, alanine, gamma aminobutyric acid, proline, and valine were 4. 15549.86 microg (r = 0.999 9), 0.595-5.95 microg (r = 0.999 8), 0.445-4.45 microg (r = 0. 999 9), 0.515-5. 15 pg (r = 0.999 9), 8.858-106.3 microg (r = 0.999 9) , 0.585-5. 85 microg (r = 0.999 8). Their average recoveries were 100.6%, 98.35%, 100.2%, 98.44%, 98.34%, 98.18% with RSD 1.8%,1.9%, 2.0%, 2.4%, 1.5% and 2.0%, respectively (n = 6). The contents of amino acids were different in samples from five productive enterprises.</p><p><b>CONCLUSION</b>The method is efficient, good reproducible, sensitive, and accurate.</p>

Determination of Toxic Elements in Radix Astragali by ICP-MS / 中国中医药信息杂志

Objective To determinate the toxic elements in Radix Astragali by ICP-MS. Methods The elements of Cu, As, Cd, Hg, Pb, Al, Cr, Sn, Ba in Radix Astragali were analyzed by ICP-MS. Results There were toxic elements in Radix Astragali. The concentration of Cu, As, Cd, Hg and Pb were not over the limits of Chinese Pharmacopoeia, but the concentration of Al, Cr and Ba were over the levels of other elements obviously. Conclusions There are difference of residues of the 9 kinds of toxic elements. It’s necessary to establish the determination methods of toxic elements (especially Al, Sn, Cr and Ba) in Chinese herbal medicines and the limits of the related toxic elements.

Comparative Study on Fingerprints of Root of Ophiopogon japonicus / 中国中医药信息杂志

Objective To establish the fingerprints for characterization of the chemical components of maidong(root of Ophiopogon japonicus) in two main cultivate regions of China,Sichuan(Chuanmaidong) and Zhejiang(Hangmaidong).Methods An HPLC-UV analytical methods was applied to detect 70% ethanol extracts of 20 samples from Sichuan and Zhejiang province,a "Fingerprint similarity evaluating system for TCM" issued by Pharmacopoeia Committee of P.R.China was used to evaluate the similarities all of the samples.Results The fingerprints revealed that the similarities were higher than 0.95 between samples from the same cultivate region,and were lower than 0.80 between samples from different regions of above two.Conclusion The fingerprints of Chuanmaidong and Hangmaidong were provided with high difference,and the difference can be taken as a most important proof for distinguishing the material medica of Chuanmaidong and Hangmaidong,but also in patent medicine of TCM.

Determination of Saccharide in Honeysuckle Extracts Produced by Three Classical Methods / 中国中医药信息杂志

Objective To determine the contents of fructose, glucose, sucrose and total saccharide in honeysuckle extracts which were produced by three classical methods, to provide scientific data for producing and controlling of those patent medicines containing honeysuckle extract. Methods Three classical extract methods were used for producing honeysuckle extracts. HPLC-ELSD method was used for determining the contents of fructose, glucose, sucrose, and a spectrophotometric determination method was used for determining the contents of total saccharide. Results The contents of fructose in three honeysuckle extracts were 5.44%, 3.47%, 4.74%, glucose were 4.35%, 2.73%, 4.21%, glucose were 17.41%, 8.47%, 14.47%, and total saccharide were 50.63%, 41.12%, 35.90%, respectively. Conclusion About 36%～51% of total components of honeysuckle extracts were determined except chlorogenic acids, and 19%～27% of components in the extracts were fructose, glucose, sucrose.

Determination of Fructose in Radix Ophiopogonis and Extracts of Radix Ophiopogonis by HPLC-ELSD / 中国中医药信息杂志

Objective To determine the content of fructose in radix ophiopogonis cropped in Sichuan and Zhejian, and in extracts of radix ophiopogonis. Methods A HPLC-ELSD method was used for determining the content of fructose. Results The content of fructose in radix ophiopogonis cropped in Sichuan and Zhejiang were 11.32%～19.96% and 3.85%～10.19%, respectively. The contents of fructose in extracts of radix ophiopogonis cropped in Sichuan and Zhejiang were 23.48%～25.82% and 21.39%～ 23.29%, respectively. Conclusion The content of fructose in radix ophiopogonis cropped in Sichuan was higher than that of in Zhejiang. The content of fructose in extracts of radix ophiopogonis cropped in Sichuan was almost the same as that of in Zhejiang.

Determination of methylophiopogonanones A and B in Radix Ophiopogonis and its extracts by HPLC / 中草药

Objective To determine the contents of methylophiopogonanone A (MOA) and methylophiopogonanone B (MOB) in Radix Ophiopogonis and its extracts. Methods An HPLC-UV method was used for determining the contents of MOA and MOB in all samples. Analytical column was Kromasil C18 (250 mm?4.6 mm, 5 ?m). Mobile phase was acetonitrle-water (55∶45) and detection wavelength was 298 nm. The flow rate of mobile phase was 1 mL/min, and temperature was 30 ℃. Results The contents of MOA in Radix Ophiopogonis cropped in Sichuan and Zhejiang Provinces were 0.004 0%-0.009 6%, 0.006 7%-0.013 4%, and the contents of MOB were 0.002 1%-0.006 2%, 0.015 9%-0.028 2%, respectively. The contents of MOA in the extract of Radix Ophiopogonis cropped in Sichuan and Zhejiang Provinces were 0.007 5%-0.008 8%, 0.011 2%-0.012 6%, and the contents of MOB were 0.003 8%-0.005 1%, 0.020 7%-0.023 8%, respectively. Conclusion The contents of MOA and MOB in Radix Ophiopogonis cropped in Zhejiang Province and its extracts are more than those in Sichuan Province and its extrouts. The method can be used for the purpose of the quality control of Radix Ophiopogonis and its extracts.