Expression of PCNA in Experimental Cholesteatoma by Implanting Autologous Free Skin Graft in Mongolian Gerbil Middle Ear Cavity / 대한이비인후과학회지

BACKGROUND AND OBJECTIVES: Cholesteatoma in the middle ear is characterized by the presence of a keratinizing epithelium which is believed to have hyperproliferative properties. Among the various approaches for evaluating proliferative activity, proliferating cell nuclear antigen (PCNA) has been recently induced as an antigenic marker of cellular proliferation. In this study, we investigated the hyperproliferative characteristic of implanted skin in Mongolian gerbil middle ear cavity by comparing its mitotic activity with that of the retroauricular skin. A secondary purpose was to provide the morphological basis for future animal studies concerning cholesteatoma pathogenesis. MATERIALS AND METHODS: Using immunohistochemical technique with anti-monoclonal antibody, we investigated PCNA expressions of the implanted free skin and normal retroauricular skin of Mongolian gerbils. RESULTS: Experimental cholesteatoma induced by implanting free skin graft showed an average PCNA labeling index of 0.47+/-0.07 and normal retroauricular skin revealed 0.14+/-0.05. The labeling index of experimental cholesteatoma was 3.47 times higher than that of normal retroauricular skin. CONCLUSION: The epithelium of experimental cholesteatoma induced by implanting free skin graft in gerbil middle ear cavity proliferates at a higher rate than its normal retroauricular skin, suggesting that this animal model can be used for future study of epithelial proliferation of cholesteatoma.

Socio-medical Survey on the Environment Workers in Jeonju Area / 예방의학회지

The author has conducted survey on the socio-medical status of th e157 entertainers (male:25, female:132) in Jeonju area, during 1 to 15 July 1974. As results of this survey, following conclusions were obtained. 1. The age distribution was revealed the peak in the age group of 20 to 24 years with 44.5 percent. Average age of male entertainers was 28.0 years and female entertainer with 23.9 years. 2. By the educational background of the entertainers, the greatest proportion or 45.9 percent was middle school, 28.7 percent in primary school. 3. Most of the entertainers had no their parents, rather of mother while the ones who had parents were 21.0 percent. 4. The average age of menarche of the female entertainers was 14.2+/-0.87 years. 5. Regarding the marital status of the entertainers, the unmarried were 81.5 percent and present status of marriage, 79.3 percent were separated. 6. The average of the first marriage was 24.4 years in male, and 22.6 years in female. 7. Regarding the working duration of the entertainers, 79.1 percent worked less than 1 year. 8. Regarding the sex experiences before marriage, 76.0 percent of the male and 91.7 percent of the female reported had such experiences. 9. Induced abortions were found to have been experienced 88.6 percent of the female entertainers. 10.Point prevalence rate for gonorrhea including non-specific arthritics of the entertainers were found 16.0 percent of the male and 17.4 percent of the female.