Assessment of the Reproducibility of Corneal Endothelial Cell Analysis with Non-Contact Specular Nicroscope

The noncontact autofocus specular microscope with incorporated semiautomated image analyzing program provide a rapid morphometric endothelial analysis. We evaluated the reproducibility of endothelial cell analysis with Konan noncon Robo-ca sp 8000 noncontact specular microscope. Specular microscopic examinations were performed three times each in twenty eyes by two examinations were performed three times one examiner. The difference of mean coefficient of variation of cell density evaluated by two examiners were not statistically significant(4.83% and 3.81, p=0.16). But, mean coefficient of variation of CV(coefficient variation of cell size, poluyimegathim) and hexagonality(pleomorphism) were statistically significantly different between two examiners(12.46%, 17.90%, p=0.04 and 12.30%, 8.40%, p=0.01, respectively). Repeatability evaluated by one examiner in two eyes showed small coefficient of variation of cell density(3.67% & 3.69%) and large coefficient of variation of CV(11.41% & 15.00%) and hexagonality(7.79% & 10.24%). In conclusion, our study has shown that Konan noncon Robo-ca sp 8000 noncontact specular microscope allows accurate estimation of endothelial cell density, But reproducibility of coefficient of variation of cell size and hexagonality analysis were lower than that of cell density analysis.

The changes of Corneal Endothelial Morphology after Phacoemulsification by using Healon GV of Viscoat

Corneal endothelial cell damage from phacoemulsification has been attributed to mechanical injury caused by surgical manipulation and oxygen free radicals produced by ultrasound. We evaluated role of protective effect in Healon GV and Viscoat on corneal endothelial cell damage during phacoemulsificatin Seventy five eyes underwent phacoemulsigication hrough scleral tunnel incision with posterior chamber lens implantation.: Healon GV, 32 eyes and Viscoat, 43 eyes. We analyzed the corneal endothelial morphology using non-contact specualr microscope and analysis program.The percent loss of corneal endothelial density at 3 months postoperative period was greater in Viscoat(5.87%) than in Healon GV(2.47%),although it was not statistically significant(p>0.1). Coefficient of variation in cell size and hexagonality of the corneal endothelial cells also showed no significant difference between the two groups(p>0.1). In conclusion, Healon GV and Viscoat have similar protective effect on the corneal endothelial cell damage induced by ultrasound during phacoemulsification.