Determination of Radix et Rhizoma Rhei in Roufuyuliecuotie by HPLC / 中国中医药信息杂志

Objective To establish a method for the determination of Radix et Rhizoma Rhei in Roufuyuliecuotie. Methods The sample was extracted with methyl and the chromatographic condition was as follows :C18 chromatographic column (250 mm?4.6 mm, 5 ?m), a mobile phase of acetonitrile-water- glacial acetic acid (60∶40∶l), the detection wavelength at 437 nm and the flow rate at 1.0 mL/min. Results A linearity of Radix et Rhizoma Rhei in Roufuyuliecuotie was obtained at range of 0.014～0.14 ?g, r =0.9996 (n =6). The average recovery was 98% and RSD=1.47% (n=6). Conclusion This method is easy, sensitive and accurate for the determination of Radix et Rhizoma Rhei in Roufuyuliecuotie.