Identification of constituents in vitro and blood-absorbed ingredients of protective effect on acute liver injury from Yin Chen Hao decoction based on UPLC-QTOF/MS / 药学学报

To identify the active constituents in vitro and blood-absorbed ingredients in vivo from Yin Chen Hao decoction provides scientific evidence for probing its prevention and treatment mechanism on acute liver injury. An ultrahigh performance liquid chromatography quadrupole-time of flight-mass spectrometry (UPLC-QTOF/MS) method was applied for analysis of Yin Chen Hao decoction and the serum samples of mice with con-A induced acute liver injury after preventive oral administration for 14 days (the use of all laboratory animals in this study was approved by the Ethics Committee of the Naval Medical University, 19YF1459400). A total of 90 chemical constituents were identified from Yin Chen Hao decoction, mainly were flavonoids, terpenoids, tannins, quinones. 5 prototype compounds were identified in the serum, including chrysophanol, deoxyrhapontin-8-O-gallate, mussaenosidic acid, herniarin, emodin. The established UPLC-QTOF/MS method could efficiently and sensitively identify the constituents in vitro and blood-absorbed ingredients of Yin Chen Hao decoction, primarily clarify the material basis of its hepatoprotective effect, and provided a scientific basis for the quality marker selection and the pharmacodynamic material basis research on the decoction.

Optimization of inclusion technology for clove oil with β-cyclodextrin polymer microsphere by uniform design / 中草药

Objective: To study the optimized technology of inclusion for the volatile oil of Caryophylli Flos with β-cyclodextrin polymer (β-CDP) microsphere. Methods β-CDP Microsphere was prepared by saturated peroxide solution. The study was carried out with uniform design to optimize the best inclusion processing conditions and inclusion compound was to characterize its structure. Results The best condition of the optimized technology of inclusion for the volatile oil of Caryophylli Flos with β-CDP microsphene was as follow: the volatile oil of Caryophylli Flos (mL)-β-CDP microsphere (g) was 1:1; inclusion temperature was 35°C; clathration time was 1 h; ethyl alcohol could be ignored. The package method is successful by characterizing structure. Conclusion: The inclusion compound of β-CDP has high rate and the inclusion process is reasonable and feasible.