Résumé
Objective@#To explore the in vitro and in vivo effect of GP73 on the proliferation, invasion and metastasis in hepatocellular carcinoma.@*Methods@#GP73 gene was knocked out using CRISPR/Cas9 gene editing system in H22 and HepG2 cells, and stable knock out strains were constructed. The knockout efficiency was measured by western blot. Colony formation assay was used to detect the effect of GP73 on long-term survival ability. Cells were then highly synchronized in G1 phase upon treatment with cell synchronization reagents (mimosine), and the percentage of cells in G2/M phase at different time points was detected by flow cytometry. The invasive and metastasis abilities of hepatocellular carcinoma cells were detected by Transwell™ assay. Furthermore, the tumor formation abilities in vivo were examined using subcutaneous xenograft models.@*Results@#The stable knock out strains of GP73 in H22 and HepG2 cells were successfully established via puromycin selection. The number of colonies of GP73 knock out groups in HepG2 and H22 cells 10 days after transfection were 400±70 and 248±60, respectively. They were significantly lower than those in the control groups (980±40 and 1 100±50, respectively; P<0.01). In addition, GP73 knockout slowed down the cell cycle progression. Moreover, the cell numbers that had migrated to the underside of the filters were 312±50 and 305±49 in the GP73 knockout groups of HepG2 and H22 cells, respectively, significantly lower than 1 540±87 and 1 270±86 in the controls (P<0.01). For transwell invasion assay, the cell number that had invaded into the underside of the filters were 230±47 and 238±54 in the GP73 knockout groups of HepG2 and H22 cells, respectively, significantly lower than 648±74 and 596±63 in the controls(P<0.01). Furthermore, the tumor volume of GP73 knockout group was (70±170) mm3, significantly smaller than (1 200±110)mm3 of the control guoup (P<0.01).@*Conclusions@#GP73 knockout decreases the proliferation, invasive and migratory abilities of HepG2 and H22 cells in vitro and in vivo. GP73 may contribute to tumorigenesis of hepatocellular carcinoma.
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Objective:To promote the application of barcode technology and establish comprehensive barcode management platform for medical equipment in order to achieve precise management of medical equipment just like ID.Methods: Combined with information management of barcode was effectively applied to the medical equipment asset; analyzed the underlying application of barcode technology in the field of equipment asset management; finally, to clarify the application effect of barcode technology in precise equipment asset management.Results: The application of barcode technology has realized dynamic management for the whole life cycle of medical equipment, and effectively improved management method and efficiency of hospital medical equipment asset.Conclusion:Through informatization to support precise management for medical equipment, and this method can form more scientific and technological barcode technology management mode for medical equipment asset. This mode can reduce the workload and difficulty for routine equipment asset management, enhance the precise management level for equipment asset, and provide a powerful technical guarantee for the reasonable and effective integration of hospital equipment assets.
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Objective To analyze the risks for medical equipment and realize risk control.Methods The medical risk management plan was proposed based on the classification of medical equipment risks. Results Hierarchical management of medical equipment was realized based on the quantitative evaluation of risk grade. The desired target was implemented, and medical equipment risk was controlled properly.Conclusion The introduction of risk management into medical equipment management benefits medical staff, medical service and patient.
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Objective: Implementation of effective control, tracking, analysis and evaluation to the hospital renovation and expansion cost and achieve the objectives of cost reduction and efficiency, environmental protection and energy saving. Methods:In practical work, insisting on the direction of cost control and environmental protection, executing accurate management, to improve energy using efficiency and reduce costs. Results:Study the means of hospital renovation and expansion project cost control and environmental protection and energy saving, improve funds usability. Conclusion:Lean management produce effectiveness, hospital renovation and expansion project cost control and environmental protection and energy conservation work can gradually get people's attention.
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Objective:The setting up of Medical equipment assets HRP information systems to achieve dynamic tracking and management of the full life cycle of the equipment assets. Methods:Regarding the equipment asset management problems in the traditional mode, introduce the HRP information system building background and describe the design and Implementation of HRP information system platform. Results:Advanced HRP information system platform ensure the management of medical equipment, shape overall operation and management mode with the main line of hospital financial management. Conclusion:Specification and strict implementation of the equipment assets HRP information system process management is the key to enhance the overall operational efficiency of hospitals.
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Objective: Through the large hospital medical equipment power supply quality of substantive, this paper aims to be the more advanced medical equipment to provide a stable security environment of power. Methods: Medical equipments for power supply system of common appeared, elaborated the medical equipment to deal with power supply quality abnormal solution countermeasures. Results: Good power supply management effectively ensure the effective performance of medical equipment. Conclusion: To provide high quality power can guarantee the medical equipment and long-term safety, reliable, stable and effective operation of the important condition.